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Application of selenized galactomannan in the preparation of anti-oxidative stress damage agent

A technology of galactomannan and antioxidants, applied in the field of application of selenized galactomannan in the preparation of anti-oxidative stress damage agents, can solve the problem of macrophage protection by selenized galactomannan Hydrogen peroxide damage and other problems, to protect cells from oxidative stress damage, reduce content, improve cell viability

Active Publication Date: 2022-07-19
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, there is no report in the prior art that selenized galactomannan protects macrophages from hydrogen peroxide damage

Method used

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  • Application of selenized galactomannan in the preparation of anti-oxidative stress damage agent
  • Application of selenized galactomannan in the preparation of anti-oxidative stress damage agent
  • Application of selenized galactomannan in the preparation of anti-oxidative stress damage agent

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Extraction and selenization modification of galactomannan, including the following steps:

[0029] (1) Extraction of galactomannan

[0030] The galactomannan-containing leguminous seeds (Tianjing) were mechanically pulverized to 20-100 mesh, added with distilled water at a solid-to-liquid ratio of 1:50, extracted at 50 °C for 24 hours, and centrifuged at 10,000 rpm for 10 minutes. , obtain a supernatant, add absolute ethanol to the supernatant to obtain a precipitate, and the precipitate is vacuum-dried to obtain a galactomannan (GM) powdery solid, and the molecular weight of galactomannan is determined by gel chromatography, and Its purity was determined by ion chromatography.

[0031] (2) Selenylation modification of galactomannan using nitric acid-sodium selenite method

[0032] First, 1% galactomannan was added to a three-necked flask containing 0.5% (v / v) sodium nitrate solution, and the GM was completely dissolved by stirring at 150 rpm for 2 h. Subsequently, s...

Embodiment 2

[0048] Selenized galactomannan on hydrogen peroxide (H 2 O 2 )-induced cellular damage

[0049] (1) Toxicity of selenized galactomannan to cells

[0050] Toxicity of six SeGMs and unmodified GM to RAW 264.7 cells was determined by co-culturing cells with samples of seven different concentrations (25, 50, 100, 200, 400, 800 μg / mL) for 24 h, and cell survival Rates were determined by CCK-8. The above solutions of different concentrations were prepared by dissolving SeGM in water.

[0051] (2)H 2 O 2 toxicity to cells

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Abstract

The invention discloses the application of selenized galactomannan in the preparation of an anti-oxidative stress damage agent, and belongs to the technical field of polymer polysaccharides. The galactomannan is treated with nitric acid-sodium selenite method to obtain selenized galactomannan. The selenized galactomannan can significantly increase H 2 O 2 After injury, the cell viability of macrophages is increased, the activity of superoxide dismutase is increased, and the content of malondialdehyde is reduced, which can obviously protect the cells in vivo from oxidative stress damage. The invention lays a theoretical foundation for the activity analysis of galactomannan selenization products and the development of antioxidant products.

Description

technical field [0001] The invention belongs to the technical field of polymer polysaccharides, in particular to the application of selenized galactomannan in the preparation of an anti-oxidative stress damage agent. Background technique [0002] In recent years, galactomannans have received more attention due to their excellent immunomodulatory effects and enhanced antioxidant capacity. Among them, the immunomodulatory effects of galactomannans are more prominent. In vitro experiments have shown that galactomannans can Promotes activation of human monocyte-derived dendritic cells. The biological activity of polysaccharides is closely related to its molecular structure. Therefore, appropriate modification and introduction of new functional groups may alter the physicochemical properties, steric structure and resulting biological activities of galactomannans. Examples of galactomannan modifications in the prior art include sulfated galactomannans and galactomannan esters, e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/736A61P39/06C08B37/00
CPCA61K31/736C08B37/0087A61P39/06
Inventor 张晓彤勇强黄曹兴陶昱恒陈雨姝廖吉丽董昊路李鑫赖晨欢
Owner NANJING FORESTRY UNIV
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