Composition with barrier repairing and light aging resisting effects, preparation method and application thereof
An anti-photoaging and composition technology, which is applied in the direction of medical preparations containing active ingredients, skin care preparations, cosmetic preparations, etc., can solve the problems of not paying attention to photoaging prevention, skin damage, water loss, etc., to achieve Good skin moisturizing effect, stable extract solution, and low extraction temperature
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experiment example 1
[0046] Cytotoxicity test:
[0047] MTT assay is an experimental method widely used in the detection of cytotoxicity or cell proliferation by measuring the number of living cells. The detection principle is that succinate dehydrogenase in the mitochondria of living cells can make the water-soluble yellow salt MTT (3- (4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide) is reduced to water-insoluble blue formazan and deposited in cells, while dead cells have no such function. The generated crystalline formazan is generally added to DMSO (dimethyl sulfoxide), and the absorbance is measured after dissolution. The specific experimental method is in a 96-well plate according to 1X104 Inoculate 100 μl of DMEM medium containing 10% bovine serum and 100 μl of keratinocytes (HaCaT) at a density of one per well, and replace with serum-free medium after 24 hours of culture. Different concentrations of the composition were added to the serum-free medium for treatment and cultured for...
experiment example 2
[0055] Antioxidant evaluation test:
[0056] DPPH, also known as 1,1-diphenyl-2-trinitrophenylhydrazine, is a very stable nitrogen-centered free radical. Its anhydrous ethanol solution is purple. When adding a free radical scavenger, the number of free radicals The absorbance becomes smaller and the color of the solution becomes lighter, which can evaluate the ability to scavenge free radicals.
[0057] The obtained composition was properly diluted, 40 μl was taken with a pipette, and 160 μl of DPPH ethanol solution (200 μg / ml) was added. After mixing, react for 30 minutes at room temperature and avoid light, then measure the absorbance A at 517nm, and set up 3 parallel experiments for each group to calculate the average value. The calculation method is as follows:
[0058] Clearance %=[1-(A 1 -A 2 ) / A 0 ×100
[0059] In the formula: A 0 For not adding the absorbance of the DPPH solution of each composition;
[0060] A 1 For the absorbance after the reaction of the DPP...
experiment example 3
[0071] Matrix metalloproteinase 1 gene expression assay:
[0072] Matrix metalloproteinase 1 Matrix metalloproteinase-1 (MMP-1) is the main enzyme responsible for the degradation of collagen, its expression will be up-regulated after being irradiated by sunlight or infrared rays, and the degradation of collagen is an important indicator of photoaging, so MMP-1 The expression is used as an index to evaluate the anti-photoaging effect.
[0073] Detect human MMP-1 by ELISA method, blank (no sample added, without IR radiation treatment, NT), negative group (no sample added, through IR radiation treatment group, IR), embodiment 8-1, 8-2 (adding samples 0.1%, 0.3%, through IR radiation treatment), embodiment 14-1, 14-2 (adding samples 0.1%, 0.3%, through IR radiation treatment), comparative example 1-1, 1-2 (adding Sample 0.1%, 0.3%, through IR radiation treatment), comparative example 2-1, 2-2 (adding sample 0.1%, 0.3%, through IR radiation treatment), comparative example 3-1, 3-2...
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