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Tissue engineering skin for promoting wound healing and preparation method thereof

A tissue engineering skin and tissue engineering technology, applied in the field of tissue engineering skin with a double-layer structure and its preparation, can solve the problems of regional necrosis, aggravation of development, and low survival rate of transplantation, and accelerate the formation and maintenance of blood vessels and epidermis Stabilize blood vessels and maintain anti-apoptotic effects

Pending Publication Date: 2021-05-25
SHANGHAI YANGPU SHIDONG HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Skin and soft tissue defects can easily lead to infection. If the treatment is not timely, it will gradually develop and aggravate, and even lead to regional necrosis
In the current clinical practice, in order to treat skin injuries, it is usually necessary to obtain skin from a healthy part of the body and transplant it to the affected area. This method not only has limited sources, but also has a low graft survival rate, and will also generate new wounds in the skin donor area during the treatment process.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Step S1: Preparation of tissue-engineered epidermis (precursor):

[0035] (a): PVA (5.0 g) was dissolved in DMSO (100 ml). GMA (0.025mol%) and DMA (1.0mol%) were added, and after stirring at 60°C for 6h, the mixture was precipitated in acetone and dried in vacuum for 2 days to obtain PVA-GMA;

[0036] (b): PVA-GMA (15g) and HA-NB (3.75g) in step (a) were added successively in 100mL PBS, stirred (200r / min) for 10 minutes, and the photoinitiator Darocur 2959 (D-2959, 0.3g) after being added to the above mixed solution, keep away from light;

[0037] Step S2: Dissolving GelMA (1 g) in 20 mL medium (89% DMEM+10% FBS+1% PS) to obtain the precursor of tissue-engineered dermis, and storing it in a sterile environment at 37°C in the dark;

[0038] Step S3: Take out the centrifuge tube loaded with cells (ADSCs transfected by Slit2) from liquid nitrogen, thaw quickly in a water bath at 37°C for 1 min, centrifuge at 800 rpm for 3 min, remove the supernatant, and add 1 mL of cell...

Embodiment 2

[0042] Apply the various steps of Example 1 to the open fracture causing skin and soft tissue defect with infection, and the debridement operation has been completed, and the rest of the steps are the same as Example 1. After one week, no secondary infection was observed at the wound; after three weeks , the growth state of the skin tissue at the defect site was good.

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Abstract

The invention relates to the field of tissue engineering skin, in particular to tissue engineering skin for promoting wound healing and a preparation method of the tissue engineering skin, and the tissue engineering skin comprises a photo-crosslinking scaffold for simulating a skin epidermal layer, a gel scaffold for simulating a skin corium layer and seed cells required by wound repair; the main components of the scaffold material for simulating the skin epidermal layer are glycidyl methacrylate modified polyvinyl alcohol and o-nitrobenzyl light trigger molecule modified hyaluronic acid; the gel scaffold for simulating the corium layer of the skin is selected from gelatin modified by methyl methacrylate; the seed cells are selected from adipose tissue-derived stem cells transfected by Slit2. The bionic skin is constructed by preparing the double-layer bionic skin tissue engineering scaffold and loading the Slit2 transfected adipose-derived mesenchymal stem cells, so that the stability and apoptosis resistance of blood vessels are favorably maintained, and the formation of the blood vessels and epidermis is accelerated, thereby promoting the healing of refractory wounds.

Description

technical field [0001] The invention relates to the technical field of tissue-engineered skin, in particular to a tissue-engineered skin with a double-layer structure for promoting wound healing and a preparation method thereof. Background technique [0002] Skin is the largest organ in the human body and an important tissue covering the body surface. The skin not only has functions such as sensation, secretion, perspiration, and respiration, but is also an important barrier between the human body and the external environment. Skin and soft tissue defects can easily lead to infection. If the treatment is not timely, it will gradually develop and aggravate, and even lead to regional necrosis. In the current clinic, in order to treat skin injuries, it is usually necessary to obtain skin from a healthy part of the body and transplant it to the affected area. This method not only has limited sources, but also has a low graft survival rate, and will also generate new wounds in t...

Claims

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Application Information

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IPC IPC(8): A61L27/16A61L27/20A61L27/22A61L27/38A61L27/50A61L27/60
CPCA61L27/16A61L27/20A61L27/222A61L27/3834A61L27/50A61L27/60A61L2400/04C08L5/08C08L29/04
Inventor 陈诚陈功刘嘉潘建锋
Owner SHANGHAI YANGPU SHIDONG HOSPITAL
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