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Polysaccharide nano composite material and preparation and application thereof

A technology of nanocomposite materials and polysaccharides, applied in wave energy or particle radiation treatment materials, drug combinations, biochemical equipment and methods, etc., can solve problems such as weak targeting, poor water solubility, and large photosensitivity of tumors

Inactive Publication Date: 2021-05-14
JIANGSU CELL TECH MEDICAL RES INST CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the technical problems such as large photosensitive reaction, weak targeting and poor water solubility in phototherapy treatment of tumors in the prior art, and provide a polysaccharide nanocomposite material with small photosensitivity, good targeting and good water solubility and Its preparation and application

Method used

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  • Polysaccharide nano composite material and preparation and application thereof
  • Polysaccharide nano composite material and preparation and application thereof
  • Polysaccharide nano composite material and preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Escherichia coli E. coli Fermentation, extraction, purification and structure identification of K5 capsular polysaccharide Heparosan.

[0038] 1, E. coli Culture and fermentation of K5

[0039] will save E. coli The K5 strain was inoculated into the seed medium (LB liquid medium) and activated by shaking overnight (37 °C, 200 rpm, 16-20 h). The glucose liquid medium was dispensed into 500 mL sterilized Erlenmeyer flasks at a volume of 100 mL / bottle, and the seed solution was inoculated into the glucose liquid medium, 1 mL per bottle. Shake and ferment at 37°C and 240 rpm for 40 h to obtain a fermentation broth.

[0040] 2. Coarse separation of Heparosan

[0041] (1) Dialysis: The fermentation broth was collected and centrifuged at 4000 g for 20 min to remove the bacterial cells to obtain the supernatant of the fermentation broth. Concentrate the supernatant of the fermentation broth by rotary steaming to an appropriate volume to obtain a concentrated solution. ...

Embodiment 2

[0053] Preparation of deacetylated Heparosan

[0054] Weigh 1 g Heparosan and dissolve it in 100 mL 2 mol / L NaOH, react at 60°C for 24 h; cool to room temperature after completion, adjust the pH to 7.0 with 2 mol / L HCL, and dialyze with a dialysis bag with a molecular weight cut-off of 3500 Da for 48 h , freeze-dried.

Embodiment 3

[0056] Ursolic acid was grafted to deacetylate Heparosan to form assembled nanomicelle HUA.

[0057] Weigh 200 mg deacetylated Heparosan and dissolve it in 50 mM MES buffer solution with a final concentration of 5 mg / mL, add 5-fold acylation reagent EDCI and NHS, stir at room temperature for 60 min in the dark, then adjust the pH to 7.0, Add ursolic acid to continue the reaction for 24 h. After the reaction, put it into a dialysis bag for dialysis for 3 days, and freeze-dry to obtain HUA.

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Abstract

The invention discloses a polysaccharide nano composite material and preparation and application thereof, and belongs to the field of development and utilization of anti-tumor nano materials. The amphoteric self-assembled nano micelle entrapped photosensitizer zinc phthalocyanine overcomes the defect that zinc phthalocyanine is insoluble in water, targeting is performed through the EPR effect of the nano material. The tumor targeting property of the zinc phthalocyanine is improved. According to the invention, the limitation that zinc phthalocyanine is applied to the traditional light therapy is broken through, and the zinc phthalocyanine derivative has an obvious inhibition effect on B16 cells and is dose-dependent.

Description

technical field [0001] The invention belongs to the field of development and utilization of anti-tumor nanomaterials, and in particular relates to a polysaccharide nanocomposite material and its preparation and application. Background technique [0002] Nanomaterials are emerging materials, which refer to materials that have at least one dimension in a three-dimensional space that is in nanometer size or consists of them as basic units. Nanomaterials have been widely used in many fields such as electronic equipment, mechanical engineering, environmental governance, cosmetics, coatings, imaging, and biomedicine. Nanomaterials can reach tumor tissue through passive targeting and active targeting. In tumor tissue, there are abundant blood vessels, wide gaps in vessel walls, poor structural integrity, lack of lymphatic return, and an osmotic retention effect, resulting in the ability of 100 nm-2 μm nanoparticles to penetrate the capillary wall of the tumor (normal tissue capill...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K9/107A61K47/36A61K47/28A61P35/00C08B37/00C12P19/26C12R1/19
CPCA61K9/1075A61K41/0071A61K47/28A61K47/36A61P35/00C08B37/0003C12P19/26
Inventor 徐红星尹鸿萍查正其
Owner JIANGSU CELL TECH MEDICAL RES INST CO LTD
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