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Mushroom foot powder preservation culture medium of hypsizigus marmoreus strain and preparation method of mushroom foot powder preservation culture medium

A technology of mushroom mushroom foot powder and mushroom mushroom foot, which is applied in the field of mushroom mushroom foot powder preservation medium and its preparation, can solve the problems of high preservation cost, environmental pollution, high pollution rate, etc., and achieve excellent preservation effect, fast growth speed, The effect of active growth metabolism

Active Publication Date: 2021-05-11
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the factory cycle production, the strains of shimeji mushrooms are generally stored on the slope of PDA medium in test tubes, and subcultured every 90 days, which has problems such as high storage cost, poor quality, and high pollution rate.
[0004] During the mushroom production process, a large number of mushroom feet will be produced. Their nutritional value is similar to that of fruiting bodies. They contain active ingredients such as polysaccharides, crude protein, crude fat, amino acids, small peptides, flavonoids, saponins and vitamins. These mushroom feet are usually regarded as Throwing away waste not only pollutes the environment, but also wastes resources

Method used

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  • Mushroom foot powder preservation culture medium of hypsizigus marmoreus strain and preparation method of mushroom foot powder preservation culture medium
  • Mushroom foot powder preservation culture medium of hypsizigus marmoreus strain and preparation method of mushroom foot powder preservation culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: air-dried shiitake mushroom foot powder preservation medium and preparation method thereof

[0027] (1) Put 200g of diced potatoes in 800ml of water, heat to boiling over high heat, simmer until it can be crushed with a glass rod, filter through 8 layers, and the filtrate is the potato extract.

[0028] (2) Transfer the potato extract obtained in step (1) into a 2L beaker, add 20 g of glucose, stir and dissolve, then add 20 g of agar powder, and heat to boiling while stirring.

[0029] (3) Add 5g of dried shiitake mushroom foot powder that has been air-dried and passed through a 100-mesh sieve while stirring, continue heating and stirring for 20 minutes, set the volume to 1L, and cool.

[0030] (4) When the temperature drops to about 90°C, divide the obtained culture medium into separate packages and sterilize at 121°C.

Embodiment 2

[0031] Embodiment 2: Dried mushroom foot powder preservation medium and preparation method thereof

[0032] (1) Put 200g of diced potatoes in 800ml of water, heat to boiling over high heat, simmer until it can be crushed with a glass rod, filter through 9 layers, and the filtrate is the potato extract.

[0033] (2) Transfer the potato extract obtained in step (1) into a 2L beaker, add 20 g of glucose, stir and dissolve, then add 20 g of agar powder, and heat to boiling while stirring.

[0034] (3) While hot, add 10 g of shiitake mushroom foot powder that has been dried and passed through a 100-mesh sieve while stirring, continue heating and stirring for 20 minutes, set the volume to 1 L, and cool.

[0035] (4) When the temperature drops to about 90°C, divide the obtained culture medium into separate packages and sterilize at 121°C.

Embodiment 3

[0036] Embodiment 3: Air-dried white jade mushroom foot powder preservation medium and preparation method thereof

[0037] (1) Put 200g of diced potatoes in 800ml of water, heat to boiling over high heat, simmer until it can be crushed with a glass rod, filter through 8 layers, and the filtrate is the potato extract.

[0038] (2) Transfer the potato extract obtained in step (1) into a 2L beaker, add 20 g of glucose, stir and dissolve, then add 20 g of agar powder, and heat to boiling while stirring.

[0039] (3) Add 15g of air-dried and 100-mesh sieve white jade mushroom foot powder while hot while stirring, continue heating and stirring for 20 minutes, dilute to 1L, and cool.

[0040] (4) When the temperature drops to about 90°C, divide the obtained culture medium into separate packages and sterilize at 121°C.

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Abstract

The invention discloses a mushroom foot powder preservation culture medium of a hypsizigus marmoreus strain and a preparation method of the mushroom foot powder preservation culture medium, and belongs to the field of strain preservation. The mushroom foot powder preservation culture medium is composed of potato extract liquid, glucose (20 g / L), agar (20 g / L), mushroom foot powder and the like, wherein the potato extract liquid is filtrate obtained after 200 g of diced potatoes are placed in 800 ml of water, heated and boiled till the diced potatoes can be squeezed to be broken through a glass rod and filtered through 8 layers of gauze, and the filtrate can be used for preparing 1 L of culture medium; and the mushroom foot powder is one of mushroom foot powder of shiitake mushroom, crab mushroom, white beech mushroom, pleurotus eryngii, hypsizygus marmoreus and other edible mushrooms. According to the preservation culture medium, the preserved hypsizigus marmoreus strain hypha is high in growth speed, high in activity and not prone to degeneration, the culture medium is low in price, the preparation method is simple and convenient, the obtained strain is high in purity, and the preservation culture medium can be widely applied to industrial production and has a remarkable market prospect.

Description

technical field [0001] The invention belongs to the field of strain preservation, and in particular relates to a culture medium for preserving mushroom foot powder of shimeji mushroom strain and a preparation method thereof. Background technique [0002] True jiji mushroom is one of the important edible fungi that has entered industrial production at present. It enjoys the reputation of "matsutake when you hear it, and jade mushroom when you eat it". It is a rare edible fungus that is rich in medicine and food. An essential amino acid, which has many functions such as improving human immunity, preventing aging, and prolonging life. Especially, the content of lysine and arginine is higher than that of ordinary mushrooms, which is helpful for adolescents to improve their intelligence, and has anti-cancer, Cholesterol-lowering effect. [0003] As an important biological resource and means of production, fungus is an indispensable and important material in the production proces...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 朱华玲林金楠班立桐黄亮韩盼盼范亚玲曾禹善王玉
Owner TIANJIN AGRICULTURE COLLEGE
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