Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Polypeptide disinfectant composition for preventing coronavirus

A coronavirus and disinfectant technology, applied in the direction of antiviral agents, active ingredients of boron compounds, active ingredients of hydroxyl compounds, etc., can solve problems such as human health endangering the economy, affecting development, and losses, and achieve good promotion and use value and low cost , the effect of a simple source

Active Publication Date: 2021-05-04
XIAMEN UNIV
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far known coronaviruses that can infect humans are HCoV-229E, HCoV-OC43, HCoV-NL63, HCoV-HKU1, SARS-CoV, MERS-CoV and 2019-nCOV, among which SARS-CoV, MERS -CoV and 2019-nCoV belong to β-coronaviruses, which have strong infectivity, are extremely harmful to human health and cause serious economic losses. Different types of coronavirus will appear alternately within ten years, which will seriously affect the development of human society
And given that there is currently no specific drug for this type of coronavirus, exploring more effective preventive measures is undoubtedly the best way to deal with coronavirus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polypeptide disinfectant composition for preventing coronavirus
  • Polypeptide disinfectant composition for preventing coronavirus
  • Polypeptide disinfectant composition for preventing coronavirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Detection of the binding ability of Peptide1&2 and its derivatives to hACE2 based on the Biacore molecular binding system

[0026] Instrument: GE Biacore T200.

[0027] Materials and reagents: Series S Sensor Chip CM5 (GE Life100530), hACE2 purified protein, peptide1&2 and its derivatives (shown in Table 1), Amine Coupling Kit (GELife100050, PBS, glycine-hydrochloric acid buffer solution (pH 2.5).

[0028] Table 1

[0029]

[0030]

[0031] experimental method:

[0032] (1) Preparation of capture surface and binding experiments

[0033] Materials: EDC, NHS, aminoethanol (amino coupling kit), PBS, hACE2, Series S Sensor ChipCM5.

[0034] Specific steps:

[0035] a. Start Biacore T200, embed Series S Sensor Chip CM5 into Biacore T200, and use degassed PBS buffer throughout the experiment;

[0036] b. According to the system requirements, add NHS, EDC, ethanolamine and hACE2 (1mg / mL) according to the volume prompted by the system, add enough amount wit...

Embodiment 2

[0048] Example 2 Using co-immunoprecipitation to illustrate the mechanism of Peptide1&2 and its derivatives and peptides inhibiting the binding of S1 protein to hACE2 under different ratios

[0049] Instrument: Westernblot electrophoresis instrument (BIO-RAD Mini- Tetra).

[0050] Materials and reagents: hACE2-His purified protein, Peptide1&2 and its derivatives, S1-HA purified protein, PBS, 5% BSA blocking solution, 1.5MTris (pH8.8), 1.0M Tris (pH6.8), 10% SDS , 10% ammonium persulfate, antibody eluent, DAB horseradish peroxidase chromogenic kit (beyotime P0202), SDS-Gly electrophoresis solution, electrotransfer solution, PVDF membrane (Beyotime, FFP28), anti-6XHis (Abcam , ab18184), anti-HA tag (Abcam, ab9110), HA-tagagarose beads (Engibody, AT0079-1mL), SDS-PAGE protein loading buffer (Beyotime, P0286-2mL).

[0051] experimental method:

[0052] (1) In vitro binding experiment of hACE2-His and S1-HA purified protein

[0053] Materials: PBS, hACE2-His, S1-HA, HA-tag aga...

Embodiment 3

[0062] A polypeptide disinfectant composition for preventing coronavirus, consisting of mixed polypeptide, honeysuckle, menthol, indigo naturalis, boric acid, glycerin and water for injection, wherein,

[0063] The mixed polypeptide is composed of the first polypeptide Peptide12, the second polypeptide Peptide13 and the third polypeptide Peptide21, the concentration of the first polypeptide Peptide12 is 21.38μM, the concentration of the second polypeptide Peptide13 is 25.30μM, and the concentration of the third polypeptide Peptide21 Concentration is 36.82 μM,

[0064] The content of honeysuckle is 17%, the content of menthol is 1.0%, the content of Qingdai is 2%, the content of boric acid is 0.15%, the content of glycerin is 15%, and the balance is water.

[0065] The preparation method of the polypeptide disinfectant composition is as follows: first dissolve honeysuckle, menthol, indigo naturalis, boric acid, and glycerin in water for injection to fully dissolve, and then con...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a polypeptide disinfectant composition for preventing coronavirus. The effective component of the polypeptide disinfectant composition comprises mixed polypeptide consisting of a first polypeptide as shown in SEQ ID NO.01, a second polypeptide as shown in SEQ ID NO.02 and a third polypeptide as shown in SEQ ID NO.03. The invention has the advantages of low cost and wide application range, and can effectively prevent coronavirus. Components of the mixed polypeptide are small peptides composed of six amino acid residues, and the mixed polypeptide is easy to produce and synthesize, low in cost, wide in application range and capable of effectively preventing coronavirus.

Description

technical field [0001] The invention belongs to the technical field of virus disinfectants, and in particular relates to a polypeptide disinfectant composition for preventing coronaviruses. Background technique [0002] Viruses of the genus Coronavirus are RNA viruses with an envelope and a linear single-stranded positive strand genome. They are a large class of viruses that exist widely in nature. So far known coronaviruses that can infect humans are HCoV-229E, HCoV-OC43, HCoV-NL63, HCoV-HKU1, SARS-CoV, MERS-CoV and 2019-nCOV, among which SARS-CoV, MERS -CoV and 2019-nCoV belong to β-coronaviruses, which have strong infectivity, are extremely harmful to human health and cause serious economic losses. Different types of coronavirus will appear alternately within ten years, which will seriously affect the development of human society. And since there is currently no specific drug for this type of coronavirus, exploring more effective preventive methods is undoubtedly the be...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/08A61K9/08A61K47/10A61P31/14A61K31/045A61K33/22
CPCA61K38/08A61K36/355A61K31/045A61K36/704A61K36/315A61K36/195A61K33/22A61K9/08A61K47/10A61K9/0019A61P31/14A61K2300/00Y02A50/30C07K7/06A01P1/00A01N37/46A01N25/00
Inventor 曾子晏胡敏煌王薛婷郑炳义曾才英曾晓玲
Owner XIAMEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com