Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of peptide disinfectant composition for preventing coronavirus

A coronavirus and disinfectant technology, applied in the directions of antiviral agents, boron compound active ingredients, hydroxyl compound active ingredients, etc., can solve problems such as affecting development, human health, economic damage, loss, etc., and achieve low cost and good promotion and use value. , the effect of simple source

Active Publication Date: 2022-05-17
XIAMEN UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far known coronaviruses that can infect humans are HCoV-229E, HCoV-OC43, HCoV-NL63, HCoV-HKU1, SARS-CoV, MERS-CoV and 2019-nCOV, among which SARS-CoV, MERS -CoV and 2019-nCoV belong to β-coronaviruses, which have strong infectivity, are extremely harmful to human health and cause serious economic losses. Different types of coronavirus will appear alternately within ten years, which will seriously affect the development of human society
And given that there is currently no specific drug for this type of coronavirus, exploring more effective preventive measures is undoubtedly the best way to deal with coronavirus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of peptide disinfectant composition for preventing coronavirus
  • A kind of peptide disinfectant composition for preventing coronavirus
  • A kind of peptide disinfectant composition for preventing coronavirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Detection of the binding ability of Peptide1&2 and its derivatives to hACE2 based on Biacore molecular binding system

[0026] Instrument: GE Biacore T200.

[0027] Materials and reagents: Series S Sensor Chip CM5 (GE Life100530), hACE2 purified protein, peptide1&2 derivatives (shown in Table 1), Amine Coupling Kit (GELife100050, PBS, glycine-hydrochloric acid buffer solution (pH 2.5).

[0028] Table 1

[0029]

[0030]

[0031] experimental method:

[0032] (1) Preparation and binding experiments of capture surface

[0033] Materials: EDC, NHS, aminoethanol (amino coupling kit), PBS, hACE2, Series S Sensor ChipCM5.

[0034] Specific steps:

[0035] a. Start Biacore T200, embed Series S Sensor Chip CM5 in Biacore T200, and use degassed PBS buffer throughout the experiment;

[0036] b. According to the system requirements, add NHS, EDC, ethanolamine and hACE2 (1mg / mL) according to the volume indicated by the system, add a sufficient amount of EP tube...

Embodiment 2

[0048] Example 2 The mechanism of inhibition of S1 protein binding to hACE2 under different ratios of Peptide1&2 and its derivatives and peptides was explained by co-immunoprecipitation

[0049] Instrument: Westernblot electrophoresis instrument (BIO-RAD Mini- Tetra).

[0050] Materials and reagents: hACE2-His purified protein, Peptide1&2 and its derivatives, S1-HA purified protein, PBS, 5% BSA blocking solution, 1.5MTris (pH8.8), 1.0M Tris (pH6.8), 10%SDS , 10% ammonium persulfate, antibody eluate, DAB horseradish peroxidase color development kit (beyotime P0202), SDS-Gly electrophoresis solution, electrotransfer solution, PVDF membrane (Beyotime, FFP28), anti-6XHis (Abcam , ab18184), anti-HA tag (Abcam, ab9110), HA-tagagarose beads (Engibody, AT0079-1mL), SDS-PAGE protein loading buffer (Beyotime, P0286-2mL).

[0051] experimental method:

[0052] (1) In vitro binding experiment between hACE2-His and S1-HA purified protein

[0053] Materials: PBS, hACE2-His, S1-HA, HA-t...

Embodiment 3

[0062] A polypeptide disinfectant composition for preventing coronavirus, consisting of mixed polypeptide, honeysuckle, menthol, indigo, boric acid, glycerin and water for injection, wherein,

[0063] The mixed polypeptide consists of the first polypeptide Peptide12, the second polypeptide Peptide13 and the third polypeptide Peptide21. The concentration of the first polypeptide Peptide12 is 21.38 μM, the concentration of the second polypeptide Peptide13 is 25.30 μM, and the third polypeptide The concentration is 36.82 μM,

[0064] The content of honeysuckle is 17%, the content of menthol is 1.0%, the content of Qingdai is 2%, the content of boric acid is 0.15%, the content of glycerin is 15%, and the balance is water.

[0065] The preparation method of the polypeptide disinfectant composition is as follows: firstly dissolving honeysuckle, menthol, indigo daisy, boric acid and glycerin in water for injection, and then fully dissolving it into an auxiliary material mother soluti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention discloses a polypeptide disinfectant composition for preventing coronavirus, its active ingredients include the first polypeptide shown in SEQ ID NO.01, the second polypeptide shown in SEQ ID NO.02 and the A mixed polypeptide composed of the third polypeptide shown in SEQ ID NO.03. The invention has low cost, wide application range and can effectively prevent coronavirus. The components of the mixed polypeptide of the present invention are all small peptides composed of six amino acid residues, easy to produce and synthesize, low in cost, wide in scope of application, and can effectively prevent coronaviruses.

Description

technical field [0001] The invention belongs to the technical field of virus disinfectants, and in particular relates to a polypeptide disinfectant composition for preventing coronavirus. Background technique [0002] Viruses of the genus Coronavirus are RNA viruses with an envelope and a linear single-stranded positive-stranded genome. They are a large class of viruses that exist widely in nature. So far, there are known coronaviruses that can infect humans, namely HCoV-229E, HCoV-OC43, HCoV-NL63, HCoV-HKU1, SARS-CoV, MERS-CoV and 2019-nCOV, among which SARS-CoV, MERS -CoV and 2019-nCoV belong to β-coronaviruses, which have extremely strong infectivity, cause great harm to human health and cause extremely serious economic losses. Different types of coronaviruses will appear alternately in ten years, which will seriously affect the development of human society. And given that there is currently no specific drug for this type of coronavirus, exploring more effective prevent...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/08A61K9/08A61K47/10A61P31/14A61K31/045A61K33/22
CPCA61K38/08A61K36/355A61K31/045A61K36/704A61K36/315A61K36/195A61K33/22A61K9/08A61K47/10A61K9/0019A61P31/14A61K2300/00Y02A50/30
Inventor 曾子晏胡敏煌王薛婷郑炳义曾才英曾晓玲
Owner XIAMEN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com