Three-dimensional micro-fluidic chip calorimeter for biochemical reaction detection and preparation method of three-dimensional micro-fluidic chip calorimeter

Abstract

The invention provides a three-dimensional micro-fluidic chip calorimeter for biochemical reaction detection and a preparation method of the three-dimensional micro-fluidic chip calorimeter. The three-dimensional micro-fluidic chip calorimeter comprises a polyimide substrate, a bismuth telluride N type semiconductor column, an antimony telluride P type semiconductor column, a thermopile surface exposed lead end, a bottom Au connection electrode, a top Au connection electrode, a Ni top protection electrode, a polyimide protection layer, a top PDMS micro-channel and a bottom PDMS micro-channel, wherein the bismuth telluride N type semiconductor column, the antimony telluride P type semiconductor column, the thermopile surface exposed lead end, the bottom Au connection electrode, the top Au connection electrode, the Ni top protection electrode, the polyimide protection layer, the top PDMS micro-channel and the bottom PDMS micro-channelare arranged on the polyimide substrate. When biological or chemical liquid is injected from the outside into the top PDMS micro-channel of the calorimeter and room-temperature pure water is injected into the bottom PDMS micro-channel, a temperature difference is formed between the two micro-channels due to a thermal reaction generated by the biological or chemical liquid, so that the upper surface of a thermopile becomes a hot node, and the lower surface of the thermopile becomes a cold node; according to the seebeck effect, a corresponding electric potential is output, and then a thermal reaction condition is obtained according to the electric potential. The calorimeter has very high sensitivity and measurement precision.

Description

technical field [0001] The invention relates to a chip calorimeter used in biochemical reaction process detection, in particular to a microfluidic chip calorimeter with a high-sensitivity three-dimensional thermopile structure and a preparation method thereof. Background technique [0002] Most chemical reactions and biological processes generate thermal signals, such as protein folding processes, enzymatic reactions, protein-nucleic acid interactions, and biomolecule-cell interactions. By measuring the heat released or absorbed by these biochemical reaction processes, the thermodynamic properties of these processes can be determined, which has important implications for protein stability and other biochemical studies. Calorimeters are fabricated based on calorimetry and are effective tools for describing chemical reactions or biological processes. It can detect the phase transition temperature and enthalpy change of the protein reaction process, and monitor the temperature...

AI Technical Summary

Problems solved by technology

However, the thermopiles currently used in biochemical microfluidic chip calorimeters are all planar and composed of simple metals, which have problems such as low sensitivity, large area, large sample consumption, and high preparation costs, and are only suitable for The measurement of large heat; while the thermal resistance heat flow sensor needs to measure the signal through the Wheatstone bridge, the test method is more complicated, and the measured signal will deviate due to the self-heating effect due to the electrothermal effect of the thermal resistance

At present, commercial calorimeters are generally prepared on rigid substrates such as silicon and silicon nitride. These materials have poor tensile strength and are prone to fracture during use.

And the thermal conductivity of these two materials is too high, which will accelerate the heat loss, resulting in a very short and unstable measurement process

At present, the microchannel system of the existing biochemical microfluidic chip calorimeter generally uses an open chamber, but based on the measurement of trace biological liquid samples, the evaporation heat loss caused by the open chamber will make the The measured solution volume cannot be precisely controlled, which will reduce the sensitivity to a certain extent

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