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Thin-layer identification method for vitality-preserving soup and similar formula extracts and preparations thereof

A thin-layer identification and extraction technology, applied in the preparation of test samples, measuring devices, instruments, etc., can solve problems such as irritation and inability to identify

Active Publication Date: 2021-04-23
浙江金城阜通制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The TLC identification method is mainly to identify the single medicinal materials according to the reference pharmacopoeia method of the medicinal flavors contained therein. The conventional TLC identification cannot identify the three medicinal materials of ginseng, astragalus and licorice at the same time, and the pre-treatment is complicated and requires ammonia treatment of ginseng, Astragalus aluminum peroxide column, etc.
In addition, in the TLC identification of ginseng and astragalus recorded in the current Chinese Pharmacopoeia, the developer contains chloroform, and in the TLC identification of licorice, the developer uses glacial acetic acid, which is harmful to the operator's eyes and nose. Has a strong stimulating effect

Method used

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  • Thin-layer identification method for vitality-preserving soup and similar formula extracts and preparations thereof
  • Thin-layer identification method for vitality-preserving soup and similar formula extracts and preparations thereof
  • Thin-layer identification method for vitality-preserving soup and similar formula extracts and preparations thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] Example 1: Preparation of Baoyuan Decoction Similar Recipe Extract

[0079] 1) Preparation of liquid extract: Take 300-400 parts by weight of ginseng, 600-800 parts by weight of astragalus, 100-200 parts by weight of licorice, 50-100 parts by weight of cinnamon, and 100-400 parts by weight of ginger, mix, pulverize, and add water Heat and reflux for extraction twice, filter, combine the two extracts, and concentrate under reduced pressure to obtain a liquid extract.

[0080] 2) Preparation of solid extract: Take the liquid extract in 1) and freeze-dry to obtain the solid extract of Baoyuan Tang and its similar recipes.

Embodiment 2

[0081] Embodiment 2: TLC identification method of Baoyuan Decoction Oral Liquid

[0082] 1) Step 1: Preparation of the test solution: take 10ml of Baoyuantang oral liquid, add 10ml of n-pentanol, ultrasonically treat for 25 minutes, let stand for 10 minutes, separate layers, absorb the supernatant, add water and wash 3 times, 15ml each time , Discard the water layer, evaporate the n-amyl alcohol solution to dryness, add 1ml of methanol to the residue to dissolve it, and use it as the test solution.

[0083]2) Step 2: preparation of reference substance solution: take ammonium glycyrrhizinate reference substance, add methanol to make 0.5 mg / ml ammonium glycyrrhizinate solution; take astragaloside IV reference substance, add methanol to make 0.5 mg / ml astragaloside IV solution; Take ginsenoside Rb1, Re and Rg1 reference substance, add methanol to make 0.5mg / ml ginsenoside Rb1, Re and Rg1 mixed solution.

[0084] 3) Step 3: Preparation of developer solution: Take ethyl acetate, f...

Embodiment 3

[0088] Embodiment 3: TLC identification method of Baoyuan Decoction tablet

[0089] 1) Step 1: Preparation of the test solution: take 2g of Baoyuantang tablet, grind it finely, add 4ml of water, stir well; add 20ml of n-amyl alcohol, sonicate for 40 minutes, let stand for 10 minutes, separate layers, and absorb the supernatant , add water to wash 4 times, 30ml each time, discard the water layer, evaporate the n-pentanol solution to dryness, add methanol 1ml to dissolve the residue, and use it as the test solution.

[0090] 2) Step 2: Preparation of reference substance solution: take ammonium glycyrrhizinate reference substance, add methanol to make 1mg / ml ammonium glycyrrhizinate solution; take astragaloside IV reference substance, add methanol to make 1mg / ml astragaloside IV solution; take ginseng Saponin Rb1, Re and Rg1 reference substance, add methanol to make 1mg / ml ginsenoside Rb1, Re and Rg1 mixed solution.

[0091] 3) Step 3: Preparation of developer solution: Take eth...

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Abstract

The invention relates to a thin-layer identification method for vitality-preserving soup and similar formula extracts and preparations thereof which can be used for identifying main medicines such as ginseng, astragalus membranaceus and liquorice in the vitality-preserving soup and the similar formula extracts and preparations thereof at the same time, avoids independent sample preparation, plate application, development, color development and inspection of three medicines, and the method is simple to operate, short in detection period and high in inspection efficiency. A thin-layer chromatogram obtained by the method is good in separation degree and clear in spot color development, and the use of irritant reagents such as ammonia water, trichloromethane and glacial acetic acid is avoided.

Description

technical field [0001] The disclosure relates to a TLC identification method for extracts and preparations of Baoyuan Decoction and its similar prescriptions, and belongs to the field of traditional Chinese medicine detection methods. Background technique [0002] Baoyuan Decoction, the recipe comes from Baoyuan Decoction in "Ancient Classic Famous Prescription Catalog (First Batch)". The medicinal flavors contained are ginseng, astragalus, licorice, cinnamon and ginger. Classic famous prescriptions refer to the prescriptions recorded in ancient TCM classics that are still widely used today, have definite curative effects, and have obvious characteristics and advantages. The state encourages product research and development based on classic famous prescriptions. [0003] The Baoyuan Decoction similar formula means that doctors of all dynasties used Baoyuan Decoction as a basis to adjust the dosage of medicines, or appropriately increase or decrease the composition of medicin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N30/94G01N1/34G01N1/38
CPCG01N30/90G01N30/94G01N1/34G01N1/38
Inventor 朱旭伟傅苗青寇强杨政周白水
Owner 浙江金城阜通制药有限公司
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