A method for making, preserving and identifying individual specimens of non-toxic rainbow trout and perch specimens
A non-toxic technique for making specimens, which is applied in the preservation of human or animal bodies, biochemical equipment and methods, and the determination/inspection of microorganisms. It can solve the problems of specimens losing their scientific research value, achieve light weight, prevent confusion, The effect of long-term use
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Embodiment 1
[0051] A kind of freshwater rainbow trout and perch (rainbow trout) prepare specimen, and concrete steps are as follows:
[0052] (1) Select a healthy rainbow trout with better body shape and less scale shedding, and suffocate the rainbow trout to death with plastic wrap. Clean the slime off the surface of the fish.
[0053] (2) Take pictures of the appearance of the rainbow trout, and measure the total length, body length, body width, body height, head length, head height, head width, snout length, eye diameter, eye distance, and tail peduncle length of the rainbow trout. The specific data are as follows 1.
[0054] (3) Fix the shape of the rainbow trout head, adjust the opening size of the fish mouth, put the rainbow trout head into the alginate impression, wait for the alginate impression to solidify completely, take the rainbow trout out of the alginate impression, The master model is finished. Make glass glue resin and putty resin mixture. The mixture is slowly poured...
Embodiment 2
[0068] A kind of seawater rainbow trout and perch (perch) prepare specimen, and concrete steps are as follows:
[0069] (1) Choose a healthy perch with better body shape and fewer scales falling off, and suffocate the fish to death with plastic wrap. Clean the slime off the surface of the bass.
[0070] (2) Take pictures of the appearance of the perch, and measure the full length, body length, body width, body height, head length, head height, head width, snout length, eye diameter, eye distance, and tail peduncle length of the sea bass. The specific data are as follows 1.
[0071] (3) Fix the shape of the perch head, adjust the opening size of the perch mouth, put the perch head into the alginate impression, wait for the alginate impression to solidify completely, take the sea bass out of the alginate impression, The master model is finished. Make glass glue resin and putty resin mixture. The mixture is slowly poured into the mother mold, so that the mixture solidifies on...
Embodiment 3
[0085] In the process of making rainbow trout stripping specimens, the muscle DNA of rainbow trout was extracted, and the DNA of the specimen was amplified by PCR with rainbow trout microsatellite primers, and the PCR products were electrophoresed on a 12% polyacrylamide gel. It records the experimental results and saves the genetic information of the specimen.
[0086] After the preparation of the specimen is completed, when there is confusion among multiple rainbow trout specimens, the fin ray DNA of the peeled specimen is extracted, and the microsatellite primers of this species are used for PCR amplification, and the PCR product is condensed in 12% polyacrylamide Run electrophoresis on the gel, record the experimental results on the gel imaging system, compare the obtained genetic information with the genetic information of the previously saved specimens, and confirm the specific individual information of the specimen, so as to realize the individual identification of the r...
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