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Preparation method of modified electrode for detecting vitamin B1 and detection method thereof

A technology for modifying electrodes and detection methods, which is applied in the field of vitamin detection, which can solve the problems of insufficient vitamin B1 concentration, low sensitivity, and high detection limit, and achieve accurate and reliable measurement results, high sensitivity, and lower detection limit.

Inactive Publication Date: 2021-03-26
合肥天一生物技术研究所有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The existing electrochemical analysis techniques for vitamin detection are mainly realized by traditional linear sweep voltammetry, conventional pulse voltammetry, differential pulse voltammetry, square wave voltammetry, stripping voltammetry, etc. Although these methods It can be used to detect vitamins, but the sensitivity is low, especially when detecting vitamin B1 in blood samples, the detection limit is often high and cannot reach the concentration level of vitamin B1 in blood samples

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  • Preparation method of modified electrode for detecting vitamin B1 and detection method thereof
  • Preparation method of modified electrode for detecting vitamin B1 and detection method thereof

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Embodiment 1

[0022] This embodiment discloses a method for preparing a modified electrode for detecting vitamin B1, comprising the following steps:

[0023] (1) Pretreatment of the base electrode: select a glassy carbon electrode or a copper electrode as the base electrode, and polish and clean the surface of the base electrode before use;

[0024] (2) Prepare the modified electrode: drip-coat or electroplate the modification solution on the surface of the base electrode prepared in step (1), and dry the modified electrode with an infrared lamp; the modification solution consists of 0.001mol / L hydrochloric acid, 0.005mol / L L of bismuth nitrate and 0.002mol / L of amino black 10B are mixed and prepared.

[0025] Among them, the polishing and cleaning treatment of the base electrode specifically includes: firstly grinding the base electrode on different types of metallographic sandpaper to make the surface of the base electrode as smooth as a mirror; μm and 0.05 μm alumina polishing powder fo...

Embodiment 2

[0028] This embodiment discloses a method for preparing a modified electrode for detecting vitamin B1, comprising the following steps:

[0029] (1) Pretreatment of the base electrode: select a glassy carbon electrode or a copper electrode as the base electrode, and polish and clean the surface of the base electrode before use;

[0030] (2) Prepare the modified electrode: drip-coat or electroplate the modification solution on the surface of the base electrode prepared in step (1), and dry the modified electrode with an infrared lamp; the modification solution consists of 0.05mol / L nitric acid, 0.01mol / L Prepared by mixing L of sodium chloride and 0.02mol / L of lead nitrate.

[0031] Among them, the polishing and cleaning treatment of the base electrode specifically includes: firstly grinding the base electrode on different types of metallographic sandpaper to make the surface of the base electrode as smooth as a mirror; μm and 0.05 μm alumina polishing powder for mirror polishi...

Embodiment 3

[0034] This embodiment discloses a method for preparing a modified electrode for detecting vitamin B1, comprising the following steps:

[0035] (1) Pretreatment of the base electrode: select a glassy carbon electrode or a copper electrode as the base electrode, and polish and clean the surface of the base electrode before use;

[0036] (2) Prepare modified electrode: drip-coat or electroplate the modified solution on the surface of the base electrode prepared in step (1), dry the modified electrode with an infrared lamp; modify the solution by 1mol / L acetic acid, 0.5mol / L It is prepared by mixing cadmium nitrate and 0.2mol / L potassium chloride.

[0037] Among them, the polishing and cleaning treatment of the base electrode specifically includes: firstly grinding the base electrode on different types of metallographic sandpaper to make the surface of the base electrode as smooth as a mirror; μm and 0.05 μm alumina polishing powder for mirror polishing; finally, place the polis...

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Abstract

The invention belongs to the technical field of vitamin detection methods, and particularly relates to a preparation method of a modified electrode for detecting vitamin B1. The method comprises the following steps: selecting a glassy carbon electrode or a copper electrode as a matrix electrode, and polishing and cleaning the surface of the matrix electrode for later use; and dropwise coating or electroplating a modification solution on the surface of the matrix electrode, and drying by using an infrared lamp to obtain the modified electrode, wherein the modification solution is composed of one or more of hydrochloric acid, nitric acid, acetic acid, bismuth nitrate, mercury nitrate, silver nitrate, potassium chloride, sodium chloride, cadmium nitrate, lead nitrate and amino black 10B, andthe concentration of each component ranges from 0.0001 mol / L to 1 mol / L. The method overcomes the defects in the prior art, has the advantages of high sensitivity, good reproducibility, convenience and quickness in operation and the like, and accurately measures the content of vitamin B1 in a blood sample.

Description

technical field [0001] The invention belongs to the technical field of vitamin detection methods, and in particular relates to a preparation method of a modified electrode for detection of vitamin B1 and a detection method thereof. Background technique [0002] As we all know, vitamins are a kind of trace organic substances that the body must ingest through food to maintain normal physiological functions, and play an important role in regulating substance metabolism and maintaining physiological functions. Among them, vitamin B1, also called thiamine, is an important biomolecule, which plays a very important catalytic role in sugar metabolism, especially on the function of the nervous system, and has a very important effect. Long-term lack or excessive intake will cause Lead to corresponding diseases, vitamin B1 deficiency can cause beriberi, multiple neuritis and other diseases, but also cheilitis, glossitis, angular cheilitis and so on. [0003] The existing methods for d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/416G01N27/48
CPCG01N27/30G01N27/308G01N27/416G01N27/48
Inventor 潘攀吴雪景何流唐涛
Owner 合肥天一生物技术研究所有限责任公司
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