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Preparation method of to-be-detected liquid of psychotropic drugs in hair

A technology for liquid and hair to be tested is applied in the preparation of test samples, measuring devices, sampling and other directions, which can solve the problems of long cracking time and cumbersome processing steps, and achieve the effects of low cost, shortened release time, and shortened preparation cycle.

Active Publication Date: 2021-03-19
HANGZHOU ALLTEST BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Its disadvantage is that its processing steps are cumbersome and the cracking time is long

Method used

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  • Preparation method of to-be-detected liquid of psychotropic drugs in hair
  • Preparation method of to-be-detected liquid of psychotropic drugs in hair
  • Preparation method of to-be-detected liquid of psychotropic drugs in hair

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Experimental program
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Effect test

preparation example Construction

[0034] A preparation method of psychotropic drug test solution in hair, comprising the steps of:

[0035](1) Hair cleaning: the functional protein-based surfactant fluorine-containing alcohol ether phosphate monoester compound and pure water alternately clean the hair for 2-4 times;

[0036] (2) Hair softening: Add the cleaned hair into the softening solution to soak and soften it for 5-10 minutes to obtain a softening product; the ratio of hair to softening solution is 100mg: 1-1.4ml; the formula of the softening solution is: Tris-HCl 0.05-0.1M, Na 2 SO 4 4-10g / L, sodium cholate 0.5-1.5g / L, Tween-20 0.05-0.15%, urea 2.5-5g / L, and the rest is pure water.

[0037] (3) Hair cracking: add cracking powder to the softened product in step (2), add grinding aid at the same time, grind and crack for 4-5 minutes to get cracking product; cracking powder formula is SDS 3.5-6.5g / L, DTT 1.25-5g / L L, mercapto-containing compound 0.15-1.0g / L, TCEP-HCl 5-10g / L;

[0038] (4) Preservation ...

Embodiment 1

[0041] A preparation method of psychotropic drug test solution in hair, comprising the steps of:

[0042] (1) Hair cleaning: the functional protein-based surfactant fluorine-containing alcohol ether phosphate monoester compound and pure water alternately clean the hair for 3 times;

[0043] (2) Hair softening: Add the cleaned hair into the softening solution to soak and soften it for 8 minutes to obtain a softening product; the ratio of hair to softening solution is 100mg: 1.2ml; the formula of the softening solution is: Tris-HCl 0.08M, Na 2 SO 4 7g / L, sodium cholate 1.0g / L, Tween-20 0.1%, urea 3.8g / L, and the rest is pure water.

[0044] (3) Hair cracking: add cracking powder to the softened product in step (2), add grinding aid at the same time, grind and crack for 4.5 minutes to get cracking product; cracking powder formula is SDS 5g / L, DTT 3g / L, mercapto-containing compound 0.12 g / L, TCEP-HCl 8g / L;

[0045] (4) Preservation of small drug molecules: add a preservation s...

Embodiment 2

[0051] A preparation method of psychotropic drug test solution in hair, comprising the steps of:

[0052] (1) Hair cleaning: the functional protein-based surfactant fluorine-containing alcohol ether phosphate monoester compound and pure water alternately clean the hair twice;

[0053] (2) Hair softening: Add the cleaned hair into the softening solution to soak and soften it for 10 minutes to obtain the softened product; the ratio of hair to softening solution is 100mg: 1ml; the formula of the softening solution is: Tris-HCl 0.05M, Na 2 SO 4 10g / L, sodium cholate 0.5g / L, Tween-20 0.05%, urea 5g / L, and the rest is pure water.

[0054] (3) Hair cracking: add cracking powder to the softened product in step (2), add grinding aid at the same time, grind and crack for 5 minutes to get cracking product; cracking powder formula is SDS 6.5g / L, DTT 5g / L, thiol-containing compound 0.15 g / L, TCEP-HCl 10g / L;

[0055] (4) Preservation of small drug molecules: add a preservation solution ...

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Abstract

The invention relates to the technical field of immunodiagnosis, discloses a preparation method of a to-be-detected liquid of psychotropic drugs in hair aiming at the problem of long cracking period of the to-be-detected liquid of psychotropic drugs in hair in the prior art, and the method comprises the following steps: (1) hair cleaning: alternately cleaning hair by using a functional protein-based surfactant and pure water; (2) hair softening: adding the cleaned hair into a softening solution for soaking and softening treatment to obtain a softened product; (3) hair cracking: adding crackingpowder into the softened product obtained in the step (2), adding a grinding aid at the same time, and grinding and cracking to obtain a pyrolysis product; and (4) drug micromolecule preservation: adding a preservation solution into the pyrolysis product obtained in the step (3) to stably preserve the drug micromolecules in the pyrolysis product so as to obtain a to-be-detected solution. The lysate provided by the invention not only enables keratin to be denatured, but also destroys the melanin structure, cleaves hair more efficiently, prolongs the detection time of the preserving fluid, hasbetter repeatability, and enables the detection result to be scientific and rigorous.

Description

technical field [0001] The invention relates to the technical field of immune analysis and detection, in particular to a method for preparing a test solution of psychotropic drugs in hair. Background technique [0002] Drug abuse seriously endangers social security and human life and health. As the number of abusers increases and becomes younger, it has become a hot topic in society, and at the same time promotes the development of in vitro diagnostic reagents. Currently, urine, saliva, and blood are the main samples for drug abuse testing. Urine testing is easy to sample but also easy to adulterate; saliva testing is easily affected by food, mouthwash, etc. will cause a sudden drop in drug concentration; usually after 48 hours of drug abuse, the basic metabolism of drugs in urine and saliva is complete, and the effective information provided by the sample Rarely, both tests have the possibility of missed detection; although blood tests can provide relatively reliable test ...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/34G01N33/558
CPCG01N1/286G01N1/30G01N1/34G01N33/946G01N33/558G01N2001/2866
Inventor 徐萌萌翟兆斌刘成王海剑
Owner HANGZHOU ALLTEST BIOTECH
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