Enteromorpha gender specific molecular marker and application thereof

Abstract

The invention belongs to the field of seaweed molecular genetics, and relates to an enteromorpha gender specific molecular marker and application thereof. The enteromorpha gender specific molecular marker is characterized in that one enteromorpha female gametophyte DNA specific molecular marker and one enteromorpha male gametophyte DNA specific molecular marker are provided, wherein the nucleotidesequences of the enteromorpha female gametophyte DNA specific molecular marker and the enteromorpha male gametophyte DNA specific molecular marker are respectively SEQ ID NO. 3 and SEQ ID NO. 4, theSEQ ID NO. 3 is obtained by amplifying a primer sequence SEQ ID NO. 1, and the SEQ ID NO. 4 is obtained by amplifying a primer sequence SEQ ID NO. 2. The specific molecular marker is completed by detecting the specific fragment through PCR (polymerase chain reaction), and the process comprises the following steps of extracting to-be-detected enteromorpha genomic DNA, performing PCR amplification,and performing agarose gel electrophoresis detection on an amplification product. The specific primer is obtained by referring to existing high-throughput data result in a laboratory, combining genomeDNA extraction, target fragment amplification and recovery, cloning by using pGEM-T vector and escherichia coli competent strain TOP10 and the like. By means of the specific molecular marker, ploidyof a large number of enteromorpha algae and gender of gametophytes can be rapidly, simply and accurately identified, and an important molecular tool is provided for enteromorpha life history researchand variety breeding.

Description

technical field [0001] The invention belongs to the field of seaweed molecular genetics, and relates to a gender-specific molecular marker of Enteromorpha and its application. Background technique [0002] Ulva prolifera belongs to Chlorophyta, Chlorophyceae, Ulvales, Ulvaceae, Ulva, is a common large green algae . Enteromorpha is rich in dietary fiber, essential amino acids, trace elements and minerals. It has high nutritional value and has edible and medicinal value. Its artificial breeding has reached a certain scale. As an emerging large economic algae, it is urgent to carry out systematic and in-depth research on its basic genetic characteristics such as life history type, reproductive mode, and algae sex, so as to lay a theoretical and methodological foundation for the hybrid breeding of Enteromorpha. [0003] There is a typical isotype alternation in the life history of Enteromorpha, which consists of haploid gametophytic generations and diploid sporophytic generati...

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Problems solved by technology

[0007] In summary, because Enteromorpha has the same type of life history type with alternation of generations and diverse reproductive methods, especially the lack of necessary genetic research accumulation, the sex identification of Enteromorpha lacks a reliable method, especially when dealing with a large number of When collecting samples, it is impossible to efficiently and accurately identify the breeding parents and offspring, which has caused great difficulties for the genetics research and hybrid breeding of Enteromorpha.

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