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Bioactive polypeptide IQIVQNEGR as well as preparation method and application thereof

A technology of biologically active peptides and derivatives, applied in the fields of peptides and proteins, can solve problems such as deficiency and transformation of human body functions, and achieve the effects of improving vitality, good anti-aging activity, and scavenging free radicals

Active Publication Date: 2021-03-16
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has been found that the lack of some peptides that regulate the physiological functions of the human body will lead to changes in the functions of the human body

Method used

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  • Bioactive polypeptide IQIVQNEGR as well as preparation method and application thereof
  • Bioactive polypeptide IQIVQNEGR as well as preparation method and application thereof
  • Bioactive polypeptide IQIVQNEGR as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The artificial synthesis of embodiment 1 active peptide IQIVQNEGR

[0026] 1. Synthesis of Bioactive Peptides

[0027] 1. Weigh 3g of RINK resin (degree of substitution: 0.3mmol / g) into a 150ml reactor and soak in 50ml of dichloromethane (DCM).

[0028] 2. After 2 hours, wash the resin with nitrogen-dimethylformamide (DMF) 3 times the volume of the resin, and then drain it. Repeat this four times, and drain the resin for use.

[0029] 3. Add a certain amount of 20% piperidine (piperidine / DMF=1:4, v:v) into the reactor, and shake it on a decolorizing shaker for 20 minutes to remove the Fmoc protecting group on the resin. After deprotection, wash four times with 3 times the resin volume of DMF, and then drain.

[0030] 4. Take a small amount of resin and use ninhydrin (Nine Wells Ninhydrin) method to detect it (two drops of test A and two drops of test B, react at 100°C for 1 min), if the resin is colored, it means that the deprotection is successful.

[0031] 5. Weigh...

Embodiment 2

[0071] Antioxidant activity experiment of embodiment 2 bioactive peptides

[0072] 1. Biologically active peptide IQIVQNEGR scavenging activity of DPPH free radicals

[0073] Prepare 0.05mg / ml DPPH solution with absolute ethanol (protect from light); add 200µl active peptide IQIVQNEGR sample and 400µl 0.05mg / ml DPPH solution to a 2ml centrifuge tube; add 50µl and 100µl active peptide IQIVQNEGR sample to a 96-well plate Water was used as the control group, and 50 µl of water and 100 µl of 0.05 mg / ml DPPH solution were added as the blank group; after standing in the dark for 30 minutes, the centrifuge tube was centrifuged, and the supernatant was added to a 96-well plate as the experimental group; at 517nm Measure the absorbance (A 对照 、A 空白 、A 样品 ) to calculate its clearance rate.

[0074] DPPH free radical scavenging rate (%)=(1- )×100%

[0075] Table 1 The activity of polypeptide IQIVQNEGR to scavenge DPPH free radicals

[0076]

[0077] It can be seen from Table 1 ...

Embodiment 3

[0090] Anti-aging activity experiment of embodiment 3 bioactive peptides

[0091] 1 Test materials and equipment

[0092] 1.1 Test animals

[0093] Oregon K wild-type Drosophila melanogaster, Experimental Center of Huazhong University of Science and Technology.

[0094] 1.2 Instruments and reagents

[0095] Instruments and equipment: Milli-Q pure water instrument; Systec H high temperature sterilization pot, Germany Systec Instrument Technology Co., Ltd.; Shanghai Jingqi LRH-80F biological incubator, Shanghai Yiheng Scientific Instrument Co., Ltd.

[0096] Reagent materials: agar powder, Sinopharm Chemical Reagent Co., Ltd.; biologically active polypeptide IQIVQNEGR obtained in Example 1.

[0097] The biologically active polypeptide IQIVQNEGR obtained in Example 1; superoxide dismutase test kit, catalase kit, Nanjing Jiancheng Biotechnology Co., Ltd.; sodium hydroxide, citric acid, disodium hydrogen phosphate, analytically pure.

[0098] Experiments were carried out with r...

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Abstract

The invention relates to bioactive polypeptide IQIVQNEGR as well as a preparation method and application thereof. An amino acid sequence of the polypeptide is Ile-Gln-Ile-Val-Gln-Asn-Glu-Gly-Arg, andin-vitro and in-vivo experiments prove that the polypeptide IQIVQNEGR has relatively good antioxidant and anti-aging biological activity, immunoregulation function and the like. The polypeptide has good application prospects in the aspects of resisting and blocking the damage of oxygen free radicals to the body, delaying the aging of the body, reducing oxidative stress injury and the like. The polypeptide has very important significance in developing foods, health care products, cosmetics and medicines with antioxidant and anti-aging functions.

Description

technical field [0001] The invention relates to the field of proteins and polypeptides, in particular to a biologically active polypeptide IQIVQNEGR and its preparation method and application. Background technique [0002] Currently, there is growing interest in health-promoting functional foods, dietary supplements and pharmaceutical preparations containing bioactive peptides derived from food proteins. Peptides have biological activities such as anti-oxidation, immune regulation, anti-tumor, cholesterol-lowering, and anti-bacterial, and can adjust and regulate various functions of the human body. Bioactive peptides usually consist of a sequence of 2 to 30 amino acids. Compared with proteins, active peptides have unique advantages, such as good solubility, high bioavailability, low gelation, low viscosity, etc., and good processing performance. With the rapid development of biotechnology, peptides have become another important field of life science research after genes. ...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12N15/11A61K8/64A61K38/08A61Q19/08A61P39/06A61P39/00A23L33/18
CPCC07K7/06A61K8/64A61Q19/08A61P39/06A61P39/00A23L33/18A61K38/00A23V2002/00A23V2200/302A23V2250/55
Inventor 肖平段金廒张森钱大玮
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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