Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of cancer cell membrane chimeric liposome nano drug delivery system

A cancer cell membrane and liposome technology, which is applied in the directions of nano-drugs, liposome delivery, nanotechnology, etc., can solve the problems of limited source of cancer cell membranes, limited large-scale preparation and application, etc., to increase the active targeting function, facilitate the Clinical application, the effect of improving anti-tumor efficiency

Active Publication Date: 2021-03-16
TIANJIN MEDICAL UNIV +1
View PDF6 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the source of cancer cell membrane is limited, which limits large-scale preparation and application
[0006] At present, the combination of molecular targeted anti-tumor therapy and immunotherapy, while directly killing tumor cells, while improving the microenvironment of tumor immunosuppression, there are few studies. The use of carrier-free nanoparticles and cell membrane chimeric drug-loaded liposome-modified nanoparticles delivery system has not been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of cancer cell membrane chimeric liposome nano drug delivery system
  • Preparation method of cancer cell membrane chimeric liposome nano drug delivery system
  • Preparation method of cancer cell membrane chimeric liposome nano drug delivery system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Preparation of chimeric liposome nano-drug delivery system (i.e. biomimetic dual-drug nanoparticles (IND CML+ZSTK NPs)) formed by prostate cancer cell membrane (PC3)

[0048] Specifically include the following steps:

[0049] (1) Under dark conditions, accurately weigh 9.58 mg of high-purity cholesterol (CHO-HP), 3.19 mg of hydrogenated soybean lecithin (HSPC), and 3.19 mg of distearoylphosphatidylethanolamine-polyethylene glycol 2000 (DSPE-mPEG2000). mg, 4.1 mg of indomethacin (IND) was added to 10 mL of absolute ethanol (protected from light), fully dissolved under ultrasonication and heating (40°C), and prepared into an ethanol solution;

[0050] (2) Evaporate the solvent with a rotary evaporator for 30 minutes, evaporate at 150rpm / min, 40°C to form a thin film, then use 10mL phosphate buffer saline pH7.4, stir at 1000rpm / min, 40°C for 30min to form a thin film Perform hydration to obtain a hydration film;

[0051] (3) Preparation of cancer cell membrane suspension...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
particle diameteraaaaaaaaaa
particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention provides a preparation method of a cancer cell membrane chimeric liposome nano drug delivery system. The preparation method comprises the following steps of: dissolving high-purity cholesterol, hydrogenated soybean phospholipid, distearoyl phosphatidyl ethanolamine-polyethylene glycol 2000 and indometacin in absolute ethyl alcohol under a dark condition to prepare an ethanol solution; carrying out rotary evaporation on the obtained ethanol solution to form a thin film; hydrating the thin film to obtain a hydrated thin film; adding a cancer cell membrane suspension solution into the hydrated thin film and extruding to obtain chimeric indometacin liposome; mixing the chimeric indometacin liposome and ZSTK474 carrier-free nanoparticles; and extruding to obtain the cancer cell membrane chimeric liposome nano drug delivery system. By adopting the preparation method of the cancer cell membrane chimeric liposome nano drug delivery system, provided by the invention, an active targeting function on tumor tissues is increased, the anti-tumor efficiency of a drug is easy to improve and the dosage of a tumor cell membrane is extremely saved; and the preparation method is convenient for clinical application.

Description

technical field [0001] The invention belongs to the technical fields of tumor targeted therapy, immunotherapy and targeted delivery, and in particular relates to a preparation method of a cancer cell membrane chimeric liposome nano drug delivery system. Background technique [0002] Tumor is one of the diseases with the highest fatality rate in the world. The occurrence of tumor is closely related to the excessive activation of PI3K signaling pathway in tumor cells. Therefore, targeting and inhibiting the PI3K signaling pathway of tumor cells can produce anti-tumor effect. The PI3K inhibitor ZSTK474 has shown good anti-tumor effect in preclinical animal experiments, but the effect of clinical trials is not satisfactory. This may be related to its distribution in other tissues other than the tumor, resulting in adverse reactions that lead to patients' intolerance and discontinuation of the drug, thereby affecting the curative effect. [0003] The non-steroidal anti-inflammat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/5377A61K47/69A61K47/46A61K9/127A61P35/00A61P35/04B82Y5/00B82Y40/00A61K31/405
CPCA61K31/5377A61K31/405A61K47/6929B82Y5/00B82Y40/00A61P35/00A61P35/04A61K9/127A61K47/46A61K2300/00
Inventor 孔德新钟玉绪赵宝全张哲杜博王冉侯岚娇于子翔
Owner TIANJIN MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com