A kind of indole-4-carbaldehyde compound with antibacterial activity in mulberry parasite, preparation method and application thereof
A technology of antibacterial activity and mulberry parasitism, applied in the field of phytochemistry, can solve the problems of unpublished antibacterial activity, etc., and achieve the effect of broadening application channels, increasing medicinal value, and wide distribution
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Embodiment 1
[0032] Embodiment 1: the preparation of the indole-4-carboxaldehyde compound that has antibacterial activity in the mulberry
[0033] Sangjishen was dried and crushed to 60 mesh, and 3 kg of the sample was extracted 3 times with ethanol aqueous solution (solid-liquid ratio 1:10) with a mass fraction of 95%, each time for 8 hours; the 3 extractions were combined, filtered, and Concentrate under pressure to a relative density of 1.10 to obtain 160 g of extract. The extract was dissolved in water at 50°C, extracted with n-butanol and dichloromethane in turn, and the dichloromethane extract was concentrated under reduced pressure to a relative density of 1.10 to obtain the extract of dichloromethane extract. Take 20 g of dichloromethane extract and dissolve it with 1 part by weight of the extract in methanol, then mix the sample with 1 part by weight of the extract of 250-mesh silica gel and perform silica gel column chromatography. Normal-phase silica gel column chromatography (...
Embodiment 2
[0034] Embodiment 2: Determination of antibacterial ability of compounds with antibacterial activity in Sangji
[0035] 1. Experimental materials and methods
[0036] 1.1 Strains and reagents: Methicillin-resistant Staphylococcus aureus ATCC43300 (Guangdong Provincial Culture Conservation Center); BHI agar medium (Guangdong Huankai Microbial Technology Co., Ltd.); MH broth medium (Guangdong Huankai Microbial Technology Co., Ltd. ); sterile TTC solution (Guangdong Huankai Microbial Technology Co., Ltd.).
[0037] 1.2 Activation of strains: resuscitate methicillin-resistant Staphylococcus aureus ATCC43300 freeze-dried bacterial powder, inoculate it into BHI medium, and culture it in a constant temperature incubator at 37°C for 12 hours. The methicillin-resistant Staphylococcus aureus was subcultured and purified by the same inoculation method for later use.
[0038] 1.3 Drug preparation: Accurately weigh 4.096g of indole-4-carboxaldehyde, dissolve it in 10mL of dimethyl sulfox...
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