Triple inactivated vaccine for Newcastle disease, H9 subtype avian influenza and avian adenovirus

A technology for chicken Newcastle disease virus and avian influenza virus, which is applied in the field of poultry vaccines, can solve the problems of long production cycle, poor vaccine purity, and risk of spreading virus, and achieves the effect of high protection rate

Pending Publication Date: 2021-02-02
成都史纪生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The production of chicken Newcastle disease virus and H9 subtype avian influenza virus from chicken embryos requires a large number of susceptible chicken embryos, and the cost is high. The virus output is easily affected by factors such as the source and quality of chicken embryos. The huge consumption of energy, after the virus is harvested, a large amount of toxic waste embryos are produced, resulting in a large amount of waste, and if the treatment is not thorough, there is a risk of dispersing the virus

Method used

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  • Triple inactivated vaccine for Newcastle disease, H9 subtype avian influenza and avian adenovirus
  • Triple inactivated vaccine for Newcastle disease, H9 subtype avian influenza and avian adenovirus
  • Triple inactivated vaccine for Newcastle disease, H9 subtype avian influenza and avian adenovirus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1 triple vaccine preparation of the present invention

[0042] 1 NDV cytotoxic preparation

[0043] AGE1 cells (duck embryonic retinal suspension cells) were gradually enlarged and passaged to a 7L bioreactor, the volume of the culture medium was 4-5L, the pH value was set to 7.15, the dissolved oxygen value was set to 60%, and the rotation speed was set to 150r / min, cultured at 37°C. When the cell density is 8.0×10 6 When the NDV aSG10 strain virus is inoculated at 0.0001 MOI, DMEM medium is added to dilute the DMEM medium concentration to 15% of the original concentration; and trypsin solution with a final concentration of 5 μg / ml is added daily. The same dose of trypsin solution was used once, the culture temperature was adjusted to 35°C, cultured for 72 hours, and samples were taken to detect the HA potency. When the HA potency was not lower than 8log2, the virus liquid was harvested. Freeze and thaw 3 times and set aside.

[0044] 2 H9 subtype avian...

Embodiment 2

[0060] Embodiment 2 triple vaccine preparation of the present invention

[0061] 1 NDV cytotoxic preparation

[0062] AGE1 cells (duck embryonic retinal suspension cells) were gradually enlarged and passaged to a 7L bioreactor, the volume of the culture medium was 4-5L, the pH value was set to 7.15, the dissolved oxygen value was set to 60%, and the rotation speed was set to 150r / min, cultured at 37°C. When the cell density is 8.0×10 6 When the NDV aSG10 strain virus is inoculated at 0.0001 MOI, DMEM medium is added to dilute the DMEM medium concentration to 10% of the original concentration; and trypsin solution with a final concentration of 3 μg / ml is added daily. The same dose of trypsin solution was used once, the culture temperature was adjusted to 36°C, cultured for 72 hours, and samples were taken to detect the HA potency. When the HA potency was not lower than 8log2, the virus liquid was harvested. Freeze and thaw 3 times and set aside.

[0063] 2 H9 subtype avian...

Embodiment 3

[0079] Embodiment 3 triple vaccine preparation of the present invention

[0080] 1 NDV cytotoxic preparation

[0081] AGE1 cells (duck embryonic retinal suspension cells) were gradually enlarged and passaged to a 7L bioreactor, the volume of the culture medium was 4-5L, the pH value was set to 7.15, the dissolved oxygen value was set to 60%, and the rotation speed was set to 150r / min, cultured at 37°C. When the cell density is 8.0×10 6 When the NDV aSG10 strain virus is inoculated at an MOI of 0.0001, DMEM medium is added to dilute the DMEM medium concentration to 20% of the original concentration; and trypsin solution with a final concentration of 7 μg / ml is added daily. The same dose of trypsin solution was used once, the culture temperature was adjusted to 37°C, cultured for 72 hours, and samples were taken to detect the HA potency. When the HA potency was not lower than 8log2, the virus liquid was harvested. Freeze and thaw 3 times and set aside.

[0082] 2H9 Subtype ...

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Abstract

The invention provides a triple inactivated vaccine for Newcastle disease, avian influenza and avian adenovirus and a preparation method of the triple inactivated vaccine, and belongs to the field ofpoultry vaccines. The preparation method comprises the following steps: taking inactivated Newcastle disease virus, H9 subtype avian influenza virus and Fiber-2 protein as active ingredients, and adding a pharmaceutically acceptable immunologic adjuvant and other auxiliary ingredients to obtain the triple inactivated vaccine, wherein the Newcastle disease virus is obtained by culturing duck embryoretina full suspension cells, and the H9 subtype avian influenza virus is obtained by culturing MDCK cells. The vaccine provided by the invention has high safety, has a protection rate superior to that of a traditional chick embryo vaccine, and has good application prospects in the vaccine industry.

Description

technical field [0001] The invention belongs to the field of poultry vaccines. Background technique [0002] Chicken Newcastle disease (ND) is one of the most harmful viral infectious diseases to the poultry industry. . The results of systematic molecular epidemiological surveys show that in recent years, the ND epidemic strains in my country are mainly genotype VII, which is significantly different from the widely used vaccine strains La Sota and Clone 30 in China. Many routine vaccine immunizations have been carried out, but there is still the possibility of infection with virulent strains causing epidemics. Clinically, the main manifestation is the occurrence of atypical ND, which proves that the immune protection of the currently used vaccines is insufficient, so the development of immunogenicity is more effective. Good vaccines that better match the current circulating strains have become the trend and urgent task of ND vaccine research and development. [0003] Since ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/17A61K39/145A61K39/235A61P31/20A61P31/14A61P31/16C12N7/00C12R1/93
CPCA61K39/12A61P31/20A61P31/14A61P31/16C12N7/00A61K2039/70A61K2039/552A61K2039/5252C12N2760/18134C12N2710/10234C12N2760/18151C12N2760/18163C12N2760/16151C12N2760/16134C12N2760/16163
Inventor 邢刚李成山丁莉岳丰雄左榕琳黄杰丁光星何洪奎王立斌王洁清廖鏖
Owner 成都史纪生物制药有限公司
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