A strain of Bacillus amyloliquefaciens and its application in the prevention and treatment of apple cropping obstacles

A technology of amylolytic bacillus and bacillus, applied in the field of agricultural microorganisms, to achieve the effect of alleviating the obstacle of repeated cropping of apples, excellent effect and increasing the content of bacteria

Active Publication Date: 2021-11-19
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are few reports on Bacillus amyloliquefaciens used to control apple cropping obstacles

Method used

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  • A strain of Bacillus amyloliquefaciens and its application in the prevention and treatment of apple cropping obstacles
  • A strain of Bacillus amyloliquefaciens and its application in the prevention and treatment of apple cropping obstacles
  • A strain of Bacillus amyloliquefaciens and its application in the prevention and treatment of apple cropping obstacles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1: Isolation and identification of bacterial strains

[0068] 1. Isolation and purification of strains:

[0069] Rhizosphere bacteria were isolated by a modified dilution spread plate method. Fresh soil samples were passed through a sieve with a diameter of 3-4 mm to remove impurities in the soil. Weigh 5 g of rhizosphere soil, add it to a triangular flask (with glass beads) filled with 45 mL of sterile water, vibrate at 180 rpm for 30 min, let it stand for 5 min, and serially dilute to 1×10 with sterile water. -4 100 μL of the supernatant of the gradient dilution was spread on the LB plate, repeated three times, and cultured upside down in a constant temperature incubator at 28°C. After a single colony grows in the plate, pick a single colony of different shapes and streak on a new LB plate for purification. At the same time, the purified strain was inoculated in liquid LB medium, shaken at 180rpm at 28°C for 12h, centrifuged at 10000r at 4°C for 10min, disc...

Embodiment 2

[0093] Embodiment 2: the broad-spectrum antibacterial effect of bacterial strain QSB-6

[0094] In order to further verify whether the QSB-6 strain has a broad-spectrum antibacterial effect on plant-derived diseases, another four common pathogenic fungi were selected for further antibacterial tests. The results are shown in Table 2.

[0095] The results of confrontation test showed that QSB-6 strain had a strong inhibitory effect on the growth of four kinds of pathogenic Fusarium mycelium. It also has a strong inhibitory effect on Penicillium citrus, Grape Anthracnose, Alternaria corn and Apple rot. Therefore, the strain QSB-6 has a broad-spectrum antibacterial effect on plant pathogenic bacteria under the conditions of the in vitro petri dish test.

[0096] Table 2: Inhibition effect of bacterial strain QSB-6 on 8 kinds of pathogenic bacteria

[0097]

[0098] Note: –, no zone of inhibition; +, zone of inhibition 10mm. Different lowercase letters indicate significant ...

Embodiment 3

[0099] Embodiment 3: the optimization of bacterial strain QSB-6 fermentation condition

[0100] 1. Test method:

[0101] The preserved strains were transferred to LB solid medium and cultured at 37°C for 24 hours. Pick the activated single colony and inoculate it into the Erlenmeyer flask containing 50ml LB liquid medium, 160r min -1 , 37 ° C shaker shaking culture until OD600 = 1 or so for use. The seed liquid is connected to the Erlenmeyer flask containing the culture medium according to the inoculation amount of 2%; at 200r·min-1 , Cultivate in a shaker at 37°C for 24h.

[0102] Fermentation culture studies with single factor experiments.

[0103] 1.1 Carbon source: use 20g·L respectively -1 Equal amounts of sucrose, maltose, glucose, lactose and soluble starch replaced the carbon source of the basal culture medium. After screening different concentrations of 0.1%, 0.5%, 1%, 1.5% and 2%, use shake flask fermentation culture, after 24h, determine the optimal carbon sour...

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Abstract

The invention discloses a strain of Bacillus amyloliquefaciens and its application in the prevention and treatment of apple cropping obstacles. The strain has been preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee on January 25, 2018, and its biological preservation number is: CGMCC NO.15307. The bacterial strain provided by the invention has inhibitory effect on various pathogenic bacteria, especially has a strong antagonistic effect on Fusarium oxysporum, Fusarium verticillium, Fusarium laminarum and Fusarium solani which cause apple cropping obstacles . The strain can also promote the growth of the aboveground and underground parts of apple tree seedlings in repeated cropping; increase the bacterial content in the soil and increase the ratio of bacteria to fungi; increase the activities of soil urease, phosphatase, sucrase and catalase in the soil for repeated cropping of apples; It has an excellent effect in alleviating the obstacle of apple replanting, and can be used for the prevention and control of apple replanting obstacle.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a strain of Bacillus amyloliquefaciens and its application in preventing and treating apple cropping obstacles. Background technique [0002] my country's apple cultivation area, total output, per capita share and export volume all rank first in the world, and China has become the world's largest apple producer and consumer. At present, my country's apple cultivation area is 37 million mu, and the apple output is about 43.8 million tons. However, the apple orchards in these traditionally advantageous cultivation areas were mainly established in the 1980s and early 1990s, and most of them have entered the aging period. In Shandong Province alone, there will be nearly 67,000 to 100,000 hm in the next five years 2 Apple orchards face renewal. Due to the limited land resources, when renewing old orchards, apple tree replanting cultivation cannot be avoided, resul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F17/20C05G3/60A01N63/22A01P3/00C12R1/07
CPCA01N63/22C05F3/00C12N1/20C05F17/20C05G3/60C12N1/205C12R2001/07C05F11/00C05F11/08Y02W30/40
Inventor 尹承苗段亚楠毛志泉刘鑫陈学森沈向
Owner SHANDONG AGRICULTURAL UNIVERSITY
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