Papillary thyroid carcinoma serum marker and application

A serum marker, papillary cancer technology, applied in the field of serum markers for papillary thyroid cancer, can solve problems such as unclear diagnosis, and achieve the effects of reducing bleeding, low cost, and improving diagnostic accuracy

Active Publication Date: 2021-01-15
CHINA JAPAN FRIENDSHIP HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, FNA remains inconclusive for 15-30% of nodules
At the same time, FNA is an invasive test and has a strong dependence on the operator's technical experience

Method used

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  • Papillary thyroid carcinoma serum marker and application
  • Papillary thyroid carcinoma serum marker and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Discovery and proof of tRF-Pro-AGG-018 molecule as a serum marker

[0022] 1. Research object

[0023] Serum samples from 43 patients with PTC, serum samples from 33 patients with benign nodules and 28 normal serum samples.

[0024] 2. Research Methods

[0025] 1. Collect serum samples: Use EDTA anticoagulant tube to collect 5 mL of cubital vein. After blood collection, gently shake the anticoagulant tube to mix the anticoagulant with the blood. After standing at 4°C for 24 hours, centrifuge at 3000×g for 5 minutes at room temperature. Use a micropipette to draw 200 μL of the upper serum and aliquot it into a new 500 μL centrifuge tube, and store it at -80°C for later use.

[0026] 2. RNA extraction

[0027] (1) Homogenate: Take out the serum sample from the -80°C refrigerator, thaw it and centrifuge at 12000×g at 4°C for 10 minutes to remove possible impurities. Take 250uL serum liquid and transfer it to a 1.5mL centrifuge tube. TRIzol LS Reagent (Invitr...

Embodiment 2

[0091] Embodiment 2: Configuration of diagnostic kit for thyroid papillary carcinoma

[0092] According to the results of Example 1, this example provides a preferred kit for the diagnosis of papillary thyroid carcinoma, which includes the following reagents:

[0093] 1. Serum RNA extraction reagent: TRIzol LS Reagent (Invitrogen life technologies), chloroform, isopropanol, 100% ethanol, 75% ethanol (prepared with DEPC-treated water), glacial acetic acid, RNase-free water (Invitrogen life technologies ), RNase-free glycogen (Invitrogen life technologies).

[0094] 2. Reverse transcription reagent: rtStar TM tRF&tiRNA Pretreatment Kit (Cat#AS-FS-005, Arraystar),

[0095] rtStar TM First-Strand cDNA Synthesis Kit (3' and 5' adapter) (Cat#AS-FS-003, Arraystar).

[0096]3. Fluorescence quantitative PCR reagent: 2X PCR master mix (Arraystar); tRF-Pro-AGG-018 specific primer: primer F: 5'-TACAGTCCGACGATCGGCTC-3', as shown in SEQ ID NO.2; primer R: 5 '-CTCTTCCGATCTCCTAGACCAAC-...

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Abstract

The invention relates to a serum tRF marker used for PTC diagnosis. Secondly, the invention provides application of a reagent used for detecting an expression quantity of the tRF in the serum in preparation of a PTC diagnosis preparation. Finally, the invention provides a PTC diagnosis kit, the PTC diagnosis kit can measure the content of the tRF in the serum, and the nucleotide sequence of the serum marker is disclosed by SEQ ID NO.1. When a tRF molecule provided by the invention and a specific primer of the tRF molecule are taken as the diagnosis reagent, the diagnosis accuracy of the PTC can be further improved, help is provided for clinical doctors to quickly master the state of an illness of a patient and formulate a diagnosis and treatment scheme, and a foundation is laid for discovering a novel small molecule targeted medicine with a potential treatment value.

Description

technical field [0001] The invention relates to the fields of bioanalysis technology and medical detection, in particular to a serum marker for papillary thyroid carcinoma and its application. Background technique [0002] In the past decade, thyroid cancer has become one of the malignant tumors with the highest incidence rate, among which papillary thyroid carcinoma (Papillary Thyroid Carcinoma, PTC) accounts for more than 95% of thyroid cancer. Accurate preoperative diagnosis is essential to reduce unnecessary thyroid surgery. At present, fine needle aspiration biopsy (Fine Needle Aspiration, FNA) is considered to be the best diagnostic tool for distinguishing benign from malignant thyroid nodules. Nevertheless, FNA still has some limitations. For example, FNA remains inconclusive for 15-30% of nodules. At the same time, FNA is an invasive examination and has a strong dependence on the operator's technical experience. Therefore, it is crucial to find a new diagnostic m...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/158
Inventor 张波汤珈嘉高琼席雪华高璐滢
Owner CHINA JAPAN FRIENDSHIP HOSPITAL
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