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Near-infrared fluorescent magnetic Fe NCs dual-mode probe as well as synthesis method and application thereof

A near-infrared and magnetic technology, applied in the fields of fluorescence/phosphorescence, chemical instruments and methods, organic materials/organic magnetic materials, etc., can solve problems such as unfavorable large-scale promotion, restricting the luminous efficiency of FeNCs probes, inability to reduce iron ions, etc. problems, to achieve the effect of novel preparation method, stable fluorescence and magnetic resonance properties, and fast reaction speed

Active Publication Date: 2021-01-05
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in this preparation method, hemoglobin is both the iron source and the preparation template. One hemoglobin molecule only contains one iron ion. If Fe NCs are prepared by this method, only one metal atom can grow on the surface of a template molecule, which greatly restricts the Fe NCs. Probe Luminescence Efficiency
At the same time, this preparation method cannot directly reduce iron ions inside the hemoglobin, and piperidine needs to be used to extract iron ions from the inside of the hemoglobin to its surface for reduction.
Piperidine is a toxic reagent and a precursor chemical, which is subject to strict control. Using piperidine as an essential reagent for the preparation of Fe NCs will face great safety and economic risks, which is not conducive to large-scale promotion in actual production.

Method used

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  • Near-infrared fluorescent magnetic Fe NCs dual-mode probe as well as synthesis method and application thereof
  • Near-infrared fluorescent magnetic Fe NCs dual-mode probe as well as synthesis method and application thereof
  • Near-infrared fluorescent magnetic Fe NCs dual-mode probe as well as synthesis method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The preparation of the near-infrared fluorescent magnetic Fe NCs is carried out according to the following steps at room temperature:

[0054] (1) Weigh 0.1000g of GSH and dissolve in 15mL of high-purity water;

[0055] (2) Weigh 0.1988g of FeCl 2 4H 2 O, dissolved in 10 mL of high-purity water to form FeCl at a concentration of 0.1M 2 Aqueous solution, pipette 1mL of the above solution into the prepared GSH solution, and fully stir for 20min;

[0056] (3) Add 0.0190 g of sodium borohydride solid to the above reaction mixed solution under stirring conditions, and then fully react under stirring for 15 minutes, the color of the reaction solution changes from colorless to light yellow, and the final product solution is obtained.

Embodiment 2

[0058] (1) The preparation method of Fe NCs refers to Example 1;

[0059] (2) Transmission electron microscopy (TEM) characterization of Fe NCs:

[0060] The prepared Fe NCs were dispersed into high-purity water, evenly drip-coated on a special copper grid, and dried to obtain observation samples, and the morphology of Fe NCs was observed by field emission transmission electron microscopy. like figure 1As shown, the morphology of the Fe NCs is approximately spherical and uniformly dispersed, and the particle size is small (about 5 nm) and uniformly distributed.

Embodiment 3

[0062] (1) The preparation method of Fe NCs refers to Example 1;

[0063] (2) Measurement of fluorescence excitation and emission spectra of Fe NCs:

[0064] The prepared Fe NCs were dispersed into high-purity water, and the fluorescence excitation spectrum and emission spectrum of the Fe NCs sample were measured by a fluorescence spectrophotometer, as shown in figure 2 As shown, the maximum excitation wavelength of the Fe NCs is 515 nm. Under the excitation of this maximum excitation wavelength, the fluorescence emission wavelength is 702 nm, which is located in the near-infrared region, indicating that the Fe NCs have good near-infrared fluorescence emission ability.

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Abstract

The invention discloses a near-infrared fluorescent magnetic Fe NCs dual-mode probe as well as a synthesis method and an application thereof. The valence state of an iron element in the magnetic Fe NCs dual-mode probe is zero, the particle size of the iron element is 5 nm, an iron nano-cluster prepared from glutathione serving as a stabilizer and iron ions in a molar ratio of 1: 0.3073 has excellent near-infrared fluorescence emission capacity, interference of biological autofluorescence in a sample can be effectively avoided, and the product can be used for high-sensitivity detection of the drug molecule tiopronin in a complex detection system. Besides, the probe also has relatively strong paramagnetic properties, can generate bright T1 weighted image signals under the action of an external magnetic field, is gradually enhanced along with the increase of the concentration of the probe, and also has a wide application prospect in the aspect of magnetic resonance imaging.

Description

[0001] The invention was supported by the National Natural Science Foundation of China (No. 21375095), Tianjin Natural Science Foundation Youth Project (No. 17JCQNJC05800), Tianjin Normal University "Inorganic-Organic Hybrid Functional Materials Chemistry Key Laboratory of Ministry of Education", "Tianjin Supported by the Open Fund Project of the Municipal Key Laboratory of Functional Molecular Structure and Performance and Tianjin Normal University's "Future Thousand Talents Program" project (WLQR201914). technical field [0002] The invention belongs to the technical field of chemical synthesis and biological analysis and detection, and relates to a magnetic iron nano-cluster with near-infrared fluorescence emission capability and a simple, green and rapid synthesis method thereof. Background technique [0003] Metal Nanoclusters (NCs), as an emerging nanoluminescent material in recent years, its particle size close to the Fermi wavelength not only makes it have excellent f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/02C09K11/60G01N21/64G01N24/08H01F1/42
CPCC09K11/025C09K11/60H01F1/42G01N21/643G01N24/08G01N2021/6432
Inventor 张菲张子彤李妍霍建中张俊华盛美思廖亚兰
Owner TIANJIN NORMAL UNIVERSITY
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