Authenticity evaluation method of Tibet sophora davidii honey
An evaluation method and authenticity technology, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as unclear basis of substance action, changes in chemical composition structure, unstable content, etc., to achieve the effect of easy operation and promotion
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Embodiment 1
[0056] In this embodiment, firstly, a large amount of Sophora japonica honey and other common honeys were sampled and analyzed by the positive and negative modes of liquid chromatography tandem high-resolution mass spectrometry. The experimental results show that there is a compound with a high content in the total ion chromatogram of the honey of Sophora japonica in the positive mode, such as figure 2 As shown, the picture A is the total ion current chromatogram of the pollen of Sophora pterosaurs, and the picture B is the precise extraction ion current chromatogram of the compound found in higher content, and its chromatographic retention time is 3.33min.
[0057] Based on the accurate mass m / z 265.19105 provided by high-resolution mass spectrometry (such as image 3 Middle A figure), the present embodiment obtains the elemental composition of this material to be C 15 h 25 N 2 o + . In the MS / MS spectrum of this substance; major fragments of peaks for this substance con...
Embodiment 2
[0062] 1. Sample source
[0063] Buy common real honey samples from the market or beekeepers to detect the content of oxymatrine in various honeys, specifically including: acacia, jujube flower, sandy locust tree, rapeseed, litchi, longan, vitex, eucalyptus, snow Four samples of fat lotus, sunflower, tallow, pomegranate, wild osmanthus, wild osmanthus, caragana, manuka, old melon head, wolfberry, codonopsis, gall, citrus honey, and duck foot wood, and the sources of the four samples are different (Origin varies and / or degree of processing varies).
[0064] 2. Solution preparation
[0065] Extraction solution: pipette 20mL of methanol, and dilute to 100mL with ultrapure water. Store at 4°C.
[0066] Oxymatrine standard solution: Weigh 10 mg of oxymatrine standard solution, and dilute to 10 mL with methanol. Store at 4°C, valid for 2 months.
[0067] 3. Q Exactive plus test conditions are as follows
[0068] (1) Sample processing
[0069] Weigh 0.8g honey and add 7.2mL ex...
Embodiment 3
[0081] 1. Sample source
[0082] A total of 20 samples of various grades or brands of honey labeled as Sophora japonica honey were purchased from the market.
[0083] 2. Experimental steps
[0084] (1) Solution preparation
[0085] Extraction solution: pipette 20mL of methanol, and dilute to 100mL with ultrapure water. Store at 4°C.
[0086] Oxymatrine standard solution: Weigh 10mg of oxymatrine standard solution, dilute to 10mL with methanol, store at 4°C, valid for 2 months.
[0087] (2) Sample processing
[0088] Weigh 0.8g honey and add 7.2mL extraction solution, vortex at room temperature until completely dissolved, and centrifuge at 20194g, 4°C for 20min. The supernatant was collected and passed through a 0.22 μm filter membrane, and then 100 μL of the extract: 900 μL of methanol water was diluted once. The diluent was transferred to a sample vial for analysis on the machine.
[0089] (3) Quality control samples
[0090] Weigh 0.8g of blank honey (without oxymatr...
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