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Preparation method of buckwheat hull non-flavonoid substance

A buckwheat hull non-flavonoid and buckwheat hull technology is applied in medical preparations, drug combinations, and pharmaceutical formulations containing active ingredients, which can solve problems such as excessive production of ROS.

Active Publication Date: 2020-12-04
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The continuous increase of blood sugar will in turn cause the excessive production of ROS

Method used

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  • Preparation method of buckwheat hull non-flavonoid substance
  • Preparation method of buckwheat hull non-flavonoid substance
  • Preparation method of buckwheat hull non-flavonoid substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Preparation of buckwheat hull non-flavonoid components

[0040] 1. Sample processing

[0041] Select natural buckwheat hulls, remove impurities, dry, crush, and pass through a 0.25 mm (60 mesh) sieve; accurately weigh 1g of buckwheat hull powder, place it in a 50mL centrifuge tube, heat water to keep warm to extract water-soluble components, filter with suction, and make the filtrate to volume to 100mL to obtain a BHE solution, which was stored for future use.

[0042] 2. Extraction of non-flavonoid components from buckwheat hulls

[0043] 1. Pretreatment of macroporous resin

[0044] Soak D101 macroporous resin and AB-8 macroporous resin in 95% ethanol for 24 h to remove impurities. Ethanol was filtered off and washed with distilled water until odorless. Soak in 4% HCl and 4% NaOH solution for 24 hours in turn, wash with distilled water to pH 7.0 after each soaking, and finally soak in 95% ethanol, save for future use.

[0045] 2. Primary purification of...

Embodiment 2

[0049] Example 2 Separation and purification of non-flavonoid substances from buckwheat hulls

[0050] 1. Separation and purification

[0051] According to the principle of similar miscibility, using the different solubility of solutes in different organic solvents, the organic impurities mixed in the non-flavonoid components of buckwheat hulls are separated; the order of polarity of the main organic reagents is: water (maximum) > acetonitrile >methanol>ethanol>propanol>acetone>tetrahydrofuran>n-butanol>ethyl acetate>diethyl ether>isopropyl ether>dichloromethane>chloroform (chloroform)>bromoethane>benzene>carbon tetrachloride>n-hexane > Kerosene (minimum); In this experiment, chloroform, ethyl acetate, n-butanol and water, which are effective for most organic substances, were selected for three-phase extraction, and according to the polarity, chloroform, ethyl acetate, and n-butanol were used in sequence to achieve The effect of synergistic extraction.

[0052] Since there a...

Embodiment 3

[0060] Example 3 Determination of non-flavonoid substances in buckwheat husk

[0061] 1. Color reaction

[0062] Using ferric chloride reaction, the BHNF-1 component and BHNF-2 component were prepared into 1 mg / mL sample solution, respectively, and the ferric chloride solution was added to react, and the color change of the solution was observed.

[0063] Using hydrochloric acid-magnesium powder reaction, the BHNF-1 component and BHNF-2 component were prepared into 1 mg / mL sample solution, and a few drops of concentrated hydrochloric acid and a small amount of magnesium powder were added to react, and the color change of the solution was observed.

[0064] Results: In the ferric chloride color reaction, the solutions of BHNF-1 and BHNF-2 components became darker and brownish black, indicating that the non-flavonoid components of buckwheat hulls may contain phenolic or tannin components. However, in the hydrochloric acid-magnesium powder test, the BHNF-1 and BHNF-2 components ...

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Abstract

The invention provides a preparation method of a buckwheat hull non-flavonoid substance. The preparation method comprises the following steps of extracting buckwheat hulls with hot water to obtain a BHE solution; conducting adsorbing by using D101 macroporous resin, conducting eluting by using ethanol, collecting eluent, and freeze-drying to obtain a D101 macroporous resin refined product; dissolving the product in 15-20 mL of water to obtain a PBHE solution, and conducting adsorbing with AB-8 type macroporous resin; washing with water; conducting eluting with an eluent, collecting the eluent,and then conducting concentrating and drying; dissolving in water again, standing, filtering, removing insoluble impurities, and adding the solution into an extraction device; adding an organic solvent, immediately conducting sufficient vibration, standing, separating liquid, and retaining components at the water layer; and concentrating and freeze-drying to obtain the buckwheat hull non-flavonoid substance. The preparation method has the advantages that the buckwheat hull non-flavonoid substance has relatively strong free radical scavenging activity and total antioxidant capacity, and has anobvious effect of protecting HepG2 cells against oxidative damages induced by H2O2; and meanwhile, the buckwheat hull non-flavonoid substance has antidiabetic activity.

Description

technical field [0001] The invention belongs to the field of health food and medicine, and in particular relates to a preparation method of buckwheat hull non-flavonoid substances. Background technique [0002] Buckwheat ( Fagopyrum esculentum ), a herbaceous dicotyledonous cereal crop, as a medicinal and edible homologous plant, has high nutritional and economic value. my country's buckwheat production ranks second in the world after Russia. Buckwheat is rich in a variety of nutritional components such as starch, protein, fat, vitamins and minerals, and the content is higher than that of bulk and small grains. It also contains many therapeutic components such as flavonoids, polyphenols, active peptides , fatty acids, phytosterols and D-chiro-inositol, etc. Epidemiology shows that regular consumption of buckwheat can effectively reduce the incidence of hyperglycemia, and it can be developed as a green functional food. Buckwheat husk, as the most abundant by-product in bu...

Claims

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Application Information

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IPC IPC(8): A61K36/70A61P3/10A61P39/06
CPCA61K36/70A61P3/10A61P39/06A61K2236/331A61K2236/55A61K2236/53A61K2236/51
Inventor 朴春红崔阳郭阳王玥刘子琦王秀娟王玉华刘俊梅于寒松代伟长
Owner JILIN AGRICULTURAL UNIV
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