Whole blood genome DNA extraction kit

Abstract

The invention relates to the technical field of a DNA extraction kit, in particular to a whole blood genome DNA extraction kit. The whole blood genome DNA extraction kit comprises lysate, an adjustingbead, a nano magnetic bead, a washing solution A, a washing solution B and an eluent; the lysate contains guanidine thiocyanate, sodium N-lauroyl sarcosinate, guanidine hydrochloride, proteinase k and Tween 20; the adjusting bead contains a semipermeable membrane balloon, and sodium chloride is put in the semipermeable membrane balloon; the nano magnetic bead is ferroferric oxide wrapping siliconoxide; the washing solution A contains hexadecyl trimethyl ammonium bromide, Tris-HCl, guanidine thiocyanate, absolute ethyl alcohol and FMES; the washing solution B contains Tris-HCl and absolute ethyl alcohol; the eluent contains Tris-HCl and disodium EDTA. When the kit is in use, the adjusting bead absorbs moisture produced in a fragmentation process and adjusts concentration of the lysate, the recovery efficiency of nucleic acid cannot be adversely affected, and accuracy of a detection result is guaranteed.

Description

technical field [0001] The invention relates to the technical field of DNA extraction kits, in particular to a whole blood genome DNA extraction kit. Background technique [0002] Nucleic acid is the carrier of genetic information, the most important biological information molecule, and the main object of molecular biology research. Therefore, nucleic acid extraction is the most important and basic operation in molecular biology experimental techniques. Nucleic acid extraction refers to the process of separating the nucleic acid from the carrier by physical and chemical methods. At present, most medical institutions and scientific research fields in China need to extract nucleic acid from a large number of blood samples. Therefore, in order to ensure that high-concentration nucleic acids are extracted, new technologies are constantly being developed. [0003] Nucleic acid extraction methods are various, including traditional phenol-chloroform method, salting-out method, me...

AI Technical Summary

Problems solved by technology

The magnetic beads used in the extraction of nucleic acid by the magnetic bead method are coated with various active groups on the surface. Studies have shown that the ionic strength, pH and other conditions in the lysate will directly affect the activity of these functional groups, and then affect the amount of nucleic acid adsorbed by the magnetic beads.

Studies have shown that when the dilution of guanidine salt lysate is reduced below 60%, the efficiency of sample DNA recovery is significantly reduced

During the hydrolysis process, the protein and other substances in the whole blood will have a certain impact on the concentration of the lysate. In the face of this situation, it is necessary to control the amount of the pre-treated sample to ensure the concentration of the guanidine salt solution and avoid excessive Dilution affects the combination of magnetic beads and nucleic acid, resulting in a decrease in nucleic acid recovery efficiency and inaccurate detection results