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Method for short-period fermentation production of low-impurity gentamicin by using micromonospora purpurea

A technology of Micromonospora cyanobacterium and gentamicin is applied in the field of short-cycle fermentation of Micromonospora cyanobacterium to produce low-impurity gentamicin, which can solve the problem of high impurities and achieve high fermentation units and short fermentation periods. , the effect of low fermentation cost

Inactive Publication Date: 2020-11-20
HEILONGJIANG GREEN HEALTH BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the defects in the above-mentioned prior art, to provide a kind of gentamicin that effectively increases the fermentation unit of gentamicin, shortens the fermentation cycle, reduces production costs, and solves the problem of high impurities in the gentamicin fermentation production. Culture medium and culture method of mycin

Method used

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  • Method for short-period fermentation production of low-impurity gentamicin by using micromonospora purpurea
  • Method for short-period fermentation production of low-impurity gentamicin by using micromonospora purpurea
  • Method for short-period fermentation production of low-impurity gentamicin by using micromonospora purpurea

Examples

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Embodiment 1

[0054] A method for producing low-impurity gentamicin by short-cycle fermentation of Micromonas rubrum, comprising the following steps:

[0055] 1) Pick gentamicin sandy soil spores (or mature mother bottle spores) with a sterile inoculation stick and culture them on the eggplant bottle slant medium for spore culture at a culture temperature of 36.5-37.5°C and a culture time of 10 days. The cultivated spore slant is stored in the refrigerator at 2-8°C for later use.

[0056] The preparation method of gentamicin eggplant bottle slant spores is:

[0057] a, the formula of eggplant bottle slant medium consists of (W / V): 0.75% cornstarch, 1.8% wheat bran (dissolving phosphorus is less than 5000PPM, starch content is less than 15%), agar 1.8%, asparagine 0.002%, 0.10% of light calcium carbonate, 0.10% of potassium nitrate, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.03% of dipotassium hydrogen phosphate, and the balance is water. g (dissolving phosphorus is less than ...

Embodiment 2

[0083] A method for producing low-impurity gentamicin by short-cycle fermentation of Micromonas rubrum, comprising the following steps:

[0084] 1) Pick gentamicin sandy soil spores (or mature mother bottle spores) with a sterile inoculation stick and culture them on the eggplant bottle slant medium for spore culture at a culture temperature of 36.5-37.5°C and a culture time of 10 days. The cultivated spore slant is stored in the refrigerator at 2-8°C for later use.

[0085] The preparation method of gentamicin eggplant bottle slant spores is:

[0086] a, the formula of eggplant bottle slant medium consists of (W / V): 0.75% cornstarch, 1.8% wheat bran (dissolving phosphorus is less than 5000PPM, starch content is less than 15%), agar 1.8%, asparagine 0.002%, 0.10% of light calcium carbonate, 0.10% of potassium nitrate, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.03% of dipotassium hydrogen phosphate, and the balance is water. g (dissolving phosphorus is less than ...

Embodiment 3

[0111] A method for producing low-impurity gentamicin by short-cycle fermentation of Micromonas rubrum, comprising the following steps:

[0112] 1) Pick gentamicin sandy soil spores (or mature mother bottle spores) with a sterile inoculation stick and culture them on the eggplant bottle slant medium for spore culture at a culture temperature of 36.5-37.5°C and a culture time of 10 days. The cultivated spore slant is stored in the refrigerator at 2-8°C for later use.

[0113] The preparation method of gentamicin eggplant bottle slant spores is:

[0114] a, the formula of eggplant bottle slant medium consists of (W / V): 0.75% cornstarch, 1.8% wheat bran (dissolving phosphorus is less than 5000PPM, starch content is less than 15%), agar 1.8%, asparagine 0.002%, 0.10% of light calcium carbonate, 0.10% of potassium nitrate, 0.05% of sodium chloride, 0.05% of magnesium sulfate, 0.03% of dipotassium hydrogen phosphate, and the balance is water. g (dissolving phosphorus is less than ...

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Abstract

The invention relates to a method for short-period fermentation production of low-impurity gentamicin by using micromonospora purpurea. The method comprises a spore slant medium, a primary seed medium, a secondary seed medium, a tertiary seed medium and a fermentation medium. The method is cheap in fermentative raw materials, short in fermentation period, high in yield and few in impurities, the problem that a downstream process is difficult to purify and low in yield is solved, and gentamicin sulfate meeting the requirements of the China Pharmacopoeia (2020 edition) is prepared.

Description

technical field [0001] The invention belongs to the field of antibiotic fermentation, and in particular relates to a method for producing low-impurity gentamicin by short-cycle fermentation of Micromonas rubrum. Background technique [0002] Gentamicin is a group of multi-component aminoglycoside antibiotics with similar structure produced by actinomycins Micromonospora rubrum and Micromonas acanthus. , C2a) mainly, and also contains sisomicin, minonomycin (C2b), gentamicin A, A1, A3, B, B1, X2 and other small components with similar structures. The activity and toxicity of each component and subcomponent are different. The quality control of gentamycin not only needs to carry out component determination, but also strictly controls the related substances in it to reduce toxic and side effects and ensure drug safety. Gentamicin fermentation medium is composed of starch, corn flour, glucose, soybean cake powder, peptone, ammonium sulfate, potassium nitrate, cobalt chloride, c...

Claims

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Application Information

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IPC IPC(8): C12P19/48C12N1/20C12R1/29
CPCC12N1/20C12P19/485
Inventor 张德重严宝冬孙明王勇
Owner HEILONGJIANG GREEN HEALTH BIOTECH
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