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Nano-drug system capable of realizing light-controlled release of CO and doxorubicin (DOX) as well as preparation and application of nano-drug system

A light-controlled release, nano-drug technology, applied in the field of biomedicine to achieve the effects of inducing respiratory metabolic disorders, simple preparation and enhanced effect

Active Publication Date: 2020-11-20
ZHEJIANG CANCER HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007]In summary, due to the complexity of drug action and preparation of carrier materials, the current combination therapy mainly focuses on the combination of multiple treatment methods (such as photothermal therapy and single chemotherapy combination of drugs), or the combination of a single chemotherapeutic drug loaded on a nano-drug delivery carrier and a specific exogenous stimulus. At present, there is no nano-drug system that combines multiple chemotherapeutic drugs and multiple therapeutic methods with high therapeutic capabilities to counteract tumor treatment for cancer

Method used

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  • Nano-drug system capable of realizing light-controlled release of CO and doxorubicin (DOX) as well as preparation and application of nano-drug system
  • Nano-drug system capable of realizing light-controlled release of CO and doxorubicin (DOX) as well as preparation and application of nano-drug system
  • Nano-drug system capable of realizing light-controlled release of CO and doxorubicin (DOX) as well as preparation and application of nano-drug system

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] The preparation of embodiment 1 PDA-DOX-CO nano

[0057] (1) At room temperature (24°C), disperse 30 mg of dopamine hydrochloride into 15 mL of double distilled water, add 120 μL of NaOH solution with a concentration of 1 mol / L to it under vigorous stirring, and rotate and stir at 1000 rpm for 5 hours Finally, the black solution was obtained and centrifuged at 400rpm for 5 minutes to separate the supernatant, which was centrifuged at 10000rpm for 5 minutes to remove the supernatant, and the resulting precipitate was PDA (polydopamine).

[0058] (2) 20mg of Mn(CO) 5 Br was dissolved in 10 mL of absolute ethanol to obtain Mn(CO) 5 Br solution; 8 mg of doxorubicin (DOX) was dissolved in 5 mL of double distilled water to obtain a DOX solution.

[0059] (3) Disperse the PDA obtained in step (1) into 15 mL of double-distilled water, and in the process of vigorous stirring, add the Mn(CO) obtained in step (2) to it 5 1mL of Br solution and 1mL of DOX solution (that is, Mn(C...

Embodiment 2

[0070] Example 2 Cell Apoptosis Experiment

[0071] The cell line used in the present invention is human colon cancer HCT-116 cells. HCT-116 cells can be purchased from the Cell Bank of Shanghai Chinese Academy of Sciences.

[0072] HCT-116 cells were cultured in a 96-well plate, 10 per well 4 cells were randomly divided into the following 5 groups (n=5):

[0073] PBS(PH 7.4)+NIR(Near Infrared), PDA+NIR(Near Infrared), PDA-CO+NIR(Near Infrared), PDA-DOX+NIR(Near Infrared), PDA-DOX-CO+NIR(Near Infrared ).

[0074] PDA is prepared by the method of step (1) of Example 1.

[0075] PDA-CO is prepared according to the following steps: PDA is prepared according to the method of step (1) of Example 1, and 20 mg of Mn(CO) 5 Br was dissolved in 10 mL of absolute ethanol to obtain Mn(CO) 5 Br solution; the above PDA was dispersed into 15mL of double distilled water, and during vigorous stirring, 1mL of Mn(CO) was added 5 After the Br solution was rotated and stirred at 1000 rpm fo...

Embodiment 3

[0082] Embodiment 3 animal experiments

[0083] The mouse tumor model was constructed as follows:

[0084] Male nude mice (Nude mice, purchased from Slack Biological Co., Ltd.) aged 6-8 weeks and weighing 20-22 g were selected as the animal model, and the HCT-116 cells in good growth were collected by centrifugation (1000 rpm, 4 minutes). And after washing with PBS (PH=7.4), the obtained cell suspension (cell density is 1×10 7 per mouse) was subcutaneously injected into the right shoulder of the mouse, and the growth of the tumor was observed and the size of the tumor was measured. The size of the tumor is measured and calculated with a vernier caliper, and the specific calculation formula is as follows: V=W 2 ×L / 2, where V represents the tumor size (volume), W is the width of the tumor, and L is the length of the tumor.

[0085] When the tumor grows to about 100mm 3 , the mice were randomly divided into 5 groups, administered by tail vein injection, and 12 hours after the...

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Abstract

The invention discloses a nano-drug system capable of realizing light-controlled release of CO and doxorubicin (DOX) as well as preparation and application of the nano-drug system. The nano-drug system takes polydopamine (PDA) as a nano-carrier, and pentacarbonyl manganese bromide and the DOX are supported on the PDA. The preparation method of the nano-drug system is simple. Dopamine hydrochlorideis dispersed into double-distilled water to obtain a dopamine hydrochloride solution, a NaOH solution is added, repeated centrifugal separation is carried out to obtain PDA, the PDA is dispersed in the double-distilled water, an ethanol solution of pentacarbonyl manganese bromide and the double-distilled water solution of DOX are added under violent stirring, rotary stirring is carried out at room temperature to obtain a black solution, a supernatant is removed by centrifugal separation, and an obtained precipitate is PDA-DOX-CO nanoparticles. The nano-drug system can controllably release theCO and the DOX under near-infrared light irradiation, effectively inhibit tumor growth, and has a good long-circulation function, biocompatibility and stability.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a nano drug system for light-controlled release of CO and doxorubicin and its preparation and application. Background technique [0002] As we all know, there is still no safe and effective treatment for malignant tumors so far. Surgical methods are difficult to completely remove tumor tissue. Traditional chemotherapy is to take orally or intravenously inject anticancer drugs, so that these anticancer drugs can reach the tumor site through the blood circulation system, so as to kill cancer cells. However, in the traditional chemotherapy treatment, mainly through intravenous administration, the small molecule drugs themselves cannot achieve specific distribution in the body, and the drugs are distributed throughout the body of the patient, killing cancer cells as well as normal tissue cells. This makes the application of small molecule chemotherapeutic drugs usually accompa...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K33/32A61K47/34A61K47/59A61P35/00A61K31/704
CPCA61K41/0052A61K33/32A61K31/704A61K47/34A61K47/59A61P35/00A61K2300/00
Inventor 沈顺刘瑶
Owner ZHEJIANG CANCER HOSPITAL
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