A Method for Separating Phospholipids in Edible Oil Based on Aminated Magnetic Hollow Composite Materials

Abstract

The invention discloses a method for separating phospholipids in edible oil based on an aminated magnetic hollow composite material, comprising the steps of: diluting the edible oil with n-hexane, adding an aminated magnetic hollow composite material, so that the phospholipids are adsorbed in the material; The mixed solution of acetic acid-ether was used to wash away the impurities adsorbed on the material, the phospholipids were eluted with methanol, and dried under nitrogen. The invention provides a method for separating phospholipids in edible oil by using a self-made magnetic hollow composite material, which improves the adsorption capacity and separation efficiency. The operation is simple, the amount of organic solvent is less, the time consumption is short, and the purification effect is good. Magnetic technology combined with hollow materials exposes phospholipid binding sites on the surface, resulting in high adsorption efficiency and short time. The magnetic material is dispersed in the oil sample. Through the active adsorption of the target substance, the separation and enrichment of phospholipids can be realized in a short time, and then the material is quickly separated by an external magnetic field, thus simplifying the pretreatment operation and improving the extraction efficiency of phospholipids.

Description

technical field [0001] The invention relates to the field of separation chemistry, and more particularly, to a method for separating phospholipids in edible oil based on aminated magnetic hollow composite materials. Background technique [0002] Phospholipids are the constituents of lipids, refer to lipids containing phosphoric acid, and belong to complex lipids. Phospholipids are the main components of biological membranes, widely distributed in nature, have high bioavailability, and play a role in metabolism and structure formation in the process of life. Phospholipids can play an important role in activating cells, maintaining metabolism, basal metabolism and balanced secretion of hormones, and enhancing the body's immunity and regeneration. The role of preventing cardiovascular diseases; phospholipids can modify biological membranes, dissolve and remove aging lipid peroxides, and play an antioxidant and anti-aging role; in addition, they can be used as solubilizers, emu...

AI Technical Summary

Problems solved by technology

[0003] Because the phospholipid hydrocarbon chain contains a large number of unsaturated bonds, it is sensitive to light and heat, and is easy to oxidize and deteriorate, which greatly increases the difficulty of phospholipid separation and purification. The currently used solid-phase extraction method for separation and purification requires a large amount of organic solvents and will reduce the separation purity. , and it is not conducive to the rapid processing of large-volume oil samples, the operation is inconvenient, and the extraction cannot be automated; and the measured value measured by ultraviolet spectrophotometry cannot fully reflect the true value

[0004] At present, adsorbents such as silica gel and aluminum oxide are usually used to separate phospholipids, but such adsorbents have problems such as small adsorption capacity, low adsorption efficiency, and poor separation purity.