Infantile nucleated red blood cell trap vector, extraction device and method

Abstract

The invention discloses an infantile nucleated red blood cell trap vector, an extraction device and a method. The infantile nucleated red blood cell trap vector is obtained by coupling a protein spinning membrane murine monoclonal antibody and a nucleated red blood cell marker murine monoclonal antibody on a protein spinning membrane. The scheme can specifically trap a nucleated red blood cell, has the characteristics of strong detection sensitivity and specificity and overcomes the defects of non-specific binding, disordered coating and low efficiency of a traditional method; the protein spinning membrane has a multi-layer porous structure, has the surface area bound with a reactor far larger than that of an existing vector, and is extremely weakly bound with non-specific ingredients in ablood sample; and furthermore, protein ingredients of the protein spinning membrane have an excellent protective effect on bound bio-active ingredients and have good stability. The extraction deviceis simple in structure and easy in preparation, is not limited by raw material source and has low cost. The extraction method is easy to operate and can avoid loss of cells in the operating process.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a fetal nucleated red blood cell capture carrier, extraction device and method. Background technique [0002] Fetal nucleated red blood cells are a kind of mononuclear cells, which exist continuously and stably in the peripheral blood of the mother from the first trimester to before delivery, containing all the genetic information of the fetus, with specific antigens on the cell surface, and a short life cycle. Prenatal testing is not affected by previous pregnancy, so fetal nucleated red blood cells are considered to be the most ideal fetal-derived cells for non-invasive prenatal diagnosis. However, the content of fetal nucleated red blood cells in maternal peripheral blood is very small, so the high-efficiency and high-purity enrichment of fetal nucleated red blood cells for clinical detection is the focus of research. [0003] The traditional separation and enrichment meth...

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Problems solved by technology

Compared with flow cytometry technology, this method is simple to operate, fast in separation speed, and low in cost, but the commonly used micron-sized magnetic beads will attract each other to a certain extent and squeeze the cells, resulting in the destruction of the target cells, and at the same time, some fetal The surface-specific antigens of nucleated red blood cells are also present in small amounts in other cells, which affects the purity of cell separation

[0006] The non-specific enrichment method has low purity because the physical and chemical properties of the target cells overlap with other blood cells, while the specific enrichment method is often costly and complicated to operate, and the antigen / antibody used in the reaction carrier is non-specific. Heterosexual and disordered adsorption on the surface of the carrier, the effective binding amount is limited, which affects the detection sensitivity, and it is difficult to meet the actual clinical needs. Therefore, there is an urgent need in this field for a new type of fetal biomarker that is simple to operate, low in cost, high in sensitivity, and strong in specificity. Nuclear Red Blood Cell Enrichment and Extraction Protocol

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