Micro-fluidic chip for detecting HPV viruses and typing

Abstract

The invention provides a micro-fluidic chip for detecting HPV (human papillomavirus) and typing. According to the micro-fluidic chip, specific primers and probes of various types are designed in an E6E7 region of an HPV gene by utilizing a high-specificity MGB probe method, and the primers, the probes and an enzyme premixed solution capable of being freeze-dried into powder are embedded into the micro-fluidic chip to obtain the micro-fluidic chip for detecting the HPV. The method has the advantages of simple operation, low cost and short period, and overcomes the problems of false positive, false positive, low efficiency, inaccurate typing and incompleteness in present universal primer-based HPV virus detection.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a microfluidic chip for detection and typing of HPV virus. Background technique [0002] Human papillomavirus (Human Paillomavirus, HPV), belonging to the family Papillomaviridae, is a small molecule, non-encapsulated, circular double-stranded DNA virus, containing about 7900 base pairs (bp), a total of 3 Gene region composition, including early region (Early Region, E region), late region (Late Region, L region) and non-coding region (UncodingRegion, UCR) or upstream regulatory region (URR). The E region consists of seven genes in sequence: E6, E7, E1, E2, E3, E4, and E5, which are involved in viral DNA replication, transcription, encoding viral proteins, and maintaining a high copy number of intracellular viruses, among which E6 and E7 It is the main oncogene of HPV, which is related to the function of virus cell transformation and carcinogenicity. HPV i...

AI Technical Summary

Problems solved by technology

The traditional early screening of female cervical cancer mainly relies on this "three-step" diagnostic procedure. When the three detection methods are used, it is easy to detach the diseased cells during the sampling process of cervical cells, and the production process is also very easy to make the cell shape abnormal. Changes or are covered by other inflammatory cells and blood cells, and the result judgment is too dependent on personal subjective judgment, so it is easy to miss the diagnosis of the disease

Of course, there are also some modern molecular detection technologies, such as DNA hybridization detection technology, Q-PCR fluorescent dye detection technology, etc. Although the detection results of DNA hybridization technology are highly specific, the detection cost and detection process are complicated, and it is very easy to make errors in the detection process. , resulting in false negative results; although the Q-PCR fluorescent dye detection technology is low in cost, it has a significant disadvantage, that is, its specificity is very susceptible to non-specific gene amplification and primer-dimer formation, which makes double-stranded DNA qPCR methods combined with fluorescent dyes have not been able to be effectively applied to clinical detection

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