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A method of treating strontium in soil by using montmorillonite/carbonate mineralization bacterial aggregates

A technology of montmorillonite and carbonate, which is applied in the field of resources and environment, can solve the problems of long remediation cycle of soil, less remediation pollution, and low efficiency of mineralization and crystallization

Active Publication Date: 2021-12-14
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most of the applications of microorganisms to remediate pollution use the adsorption mechanism of microorganisms, but there are few reports on the problem of remediation of pollution by carbonate mineralization bacteria ore-forming technology. At the same time, the mineralization crystallization efficiency of existing carbonate mineralization bacteria is low. Longer cycle time when remediating soil

Method used

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  • A method of treating strontium in soil by using montmorillonite/carbonate mineralization bacterial aggregates
  • A method of treating strontium in soil by using montmorillonite/carbonate mineralization bacterial aggregates

Examples

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Embodiment 1

[0027] A method montmorillonite / carbonate mineral aggregates treated soil bacteria using strontium, comprising the steps of:

[0028] After S1. The 821 nuclear radioactive contamination of soil samples collected into 0.9% saline for 25 ℃, 24h shaking cultivation, selection medium was applied to plates and incubated 30h at 28 ℃, the microorganisms grow to be employed streak plate on microbial purification; purified to over 4 generations microorganism inoculated in enriched media, at 28 ℃, shaking at 180rpm incubated for 2 days, according to the growth of microorganisms, selected from a carbon enriched media strain mineralized salt; the filter medium comprises the following parts by weight of the starting material: 0.8g peptone, 1.8 g of potassium dihydrogen phosphate, 4.5 g of sodium chloride, 0.8 g of glucose, 15g of urea, 0.25 g beef powder, addition of the sterile water pH adjusted to 7.0 selective medium; said enrichment medium comprising the following parts by weight of the ...

Embodiment 2

[0032] A method montmorillonite / carbonate mineral aggregates treated soil bacteria using strontium, comprising the steps of:

[0033] After S1. The 821 nuclear radioactive contamination of soil samples collected into 0.9% saline for 28 ℃, 32h shaking cultivation, selection medium was applied to plates and incubated 30h at 28 ℃, the microorganisms grow to be employed streak plate on microbial purification; purified to over 4 generations microorganism inoculated in enriched media, at 28 ℃, incubated shaking at 180rpm for 2.5 days. the microorganism growth, selected from a carbon enriched media strain mineralized salt; the filter medium comprises the following parts by weight of the starting material: 1.0g peptone, 2.0 g of potassium dihydrogen phosphate, 5.0 g sodium chloride, 1.0 g of glucose, urea 16G, 0.3 g of beef powder, addition of the sterile water adjusted to pH 7.1 selective medium; said enrichment medium comprising the following parts by weight of the starting material: ...

Embodiment 3

[0037] Method for treating soil in the soil with montmorillonite / carbonate mineralized bacteria, including the following steps:

[0038] S1. Put the acquired medium-core 821 radioactive contamination soil sample in 0.9% physiological saline for 30 ° C, 48h oscillating, coated into the screening medium plate, cultured at 28 ° C for 30 h, to be used for microorganisms The microbial is purified by the microorganisms in a flat panel; the microorganisms purified to 4 or more are inoculated in the enrichment medium, and 3 days of oscillating at 28 ° C, 180 rpm, according to the microbial growth, sutoff from the enrichment medium Saltite strain; the screening medium includes raw materials of the following weight: 1.2 g of protein, 2.2 g of dihydrogen phosphate, 5.5 g sodium chloride, 1.2 g of glucose, 18g urea, 0.33g beef powder, add sterile Water will screen the medium pH to 7.5; the enrichment medium includes a raw material of the following weight: 1.5 g of protein, 6.0 g sodium chlo...

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Abstract

The invention discloses a method for treating strontium in soil by using montmorillonite / carbonate mineralizing bacteria aggregates, and belongs to the technical field of resources and environment. The invention adopts radioactive soil to cultivate carbonate mineralization strains, and then expands the carbonate mineralization strains and montmorillonite with good anti-seepage, airtightness, cementation, solidification and adsorption functions, and centrifuges to obtain montmorillonite Delithification / carbonate mineralization bacteria aggregates, the montmorillonite / carbonate mineralization bacteria aggregates and mixture are added to the strontium-containing soil by adding batches for several times, and then added to the strontium-containing soil, and then added to the hydrochloric acid sodium chloride solution Hydrochloric acid adjusts soil pH and nucleation of sodium chloride, mineralizes, seals and solidifies strontium in situ, so as to block the migration and diffusion of strontium in soil from the source.

Description

technical field [0001] The invention belongs to the technical field of resources and environment, and in particular relates to a method for treating strontium in soil by utilizing montmorillonite / carbonate mineralizing bacteria aggregates. Background technique [0002] With the continuous development of science and technology, the problem of environmental pollution is becoming more and more serious, seriously affecting people's health and quality of life. A series of activities such as the mining industry, national defense scientific research activities, radioactive waste disposal, and the opening of nuclear weapon test bases have led to increasingly serious problems of environmental radioactive pollution. Among them, the treatment and remediation of contaminated soil containing radionuclides has attracted increasing attention. [0003] At present, the methods for treating polluted soil include physical treatment, chemical treatment, engineering treatment and biological tre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/08B09C1/10C12N1/20C12N11/14C12R1/07
CPCB09C1/08B09C1/10C12N1/20C12N11/14B09C2101/00
Inventor 竹文坤李显寅贠朝乐董云陈涛
Owner SOUTHWEAT UNIV OF SCI & TECH
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