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Method for treating strontium in soil by utilizing montmorillonite/carbonate mineralizing bacteria aggregate

A montmorillonite and carbonate technology, which is applied in the fields of resources and environment, can solve the problems of low mineralization crystallization efficiency, long soil remediation cycle, and few remediation pollution problems.

Active Publication Date: 2020-11-03
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, most of the applications of microorganisms to remediate pollution use the adsorption mechanism of microorganisms, but there are few reports on the problem of remediation of pollution by carbonate mineralization bacteria ore-forming technology. At the same time, the mineralization crystallization efficiency of existing carbonate mineralization bacteria is low. Longer cycle time when remediating soil

Method used

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  • Method for treating strontium in soil by utilizing montmorillonite/carbonate mineralizing bacteria aggregate
  • Method for treating strontium in soil by utilizing montmorillonite/carbonate mineralizing bacteria aggregate

Examples

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Effect test

Embodiment 1

[0027] A method utilizing montmorillonite / carbonate mineralizing bacterial aggregates to process strontium in soil, comprising the following steps:

[0028] S1. Put the collected Zhonghe 821 radioactively contaminated soil samples into 0.9% physiological saline for 25°C and 24h shaking cultivation, spread them on the screening medium plate, and cultivate them at 28°C for 30h, and use them after the microorganisms grow out Purify the microorganisms by streaking on the plate; inoculate the microorganisms purified to more than 4 generations in the enrichment medium, shake and cultivate them at 28°C and 180rpm for 2 days, and screen the carbon from the enrichment medium according to the growth of the microorganisms. salt mineralization strain; the screening medium includes the following raw materials by weight: 0.8g peptone, 1.8g potassium dihydrogen phosphate, 4.5g sodium chloride, 0.8g glucose, 15g urea, 0.25g beef powder, add sterile water to adjust the pH of the screening medi...

Embodiment 2

[0032] A method utilizing montmorillonite / carbonate mineralizing bacterial aggregates to process strontium in soil, comprising the following steps:

[0033] S1. Put the collected Zhonghe 821 radioactively contaminated soil samples into 0.9% physiological saline for 28°C and 32h shaking cultivation, then spread them on the screening medium plate, and cultivate them at 28°C for 30h, and use them after the microorganisms grow out. Purify the microorganisms by streaking on the plate; inoculate the microorganisms purified to more than 4 generations in the enrichment medium, shake and cultivate them at 28°C and 180rpm for 2.5 days, and screen the carbon from the enrichment medium according to the growth of the microorganisms. salt mineralization strain; the screening medium includes the following raw materials in parts by weight: 1.0g peptone, 2.0g potassium dihydrogen phosphate, 5.0g sodium chloride, 1.0g glucose, 16g urea, 0.3g beef powder, add sterile water to adjust the pH of th...

Embodiment 3

[0037] A method utilizing montmorillonite / carbonate mineralizing bacterial aggregates to process strontium in soil, comprising the following steps:

[0038] S1. Put the collected Zhonghe 821 radioactively contaminated soil samples into 0.9% physiological saline for 30°C and 48h shaking cultivation, spread them on the screening medium plate, and cultivate them at 28°C for 30h, and use them after the microorganisms grow out Purify the microorganisms by streaking on the plate; inoculate the microorganisms purified to more than 4 generations in the enrichment medium, shake and cultivate them at 28°C and 180rpm for 3 days, and screen the carbon from the enrichment medium according to the growth of the microorganisms. salt mineralization bacterial strain; the screening medium includes the following raw materials in parts by weight: 1.2g peptone, 2.2g potassium dihydrogen phosphate, 5.5g sodium chloride, 1.2g glucose, 18g urea, 0.33g beef powder, add aseptic water to adjust the pH of...

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Abstract

The invention discloses a method for treating strontium in soil by utilizing montmorillonite / carbonate mineralizing bacterium aggregates, and belongs to the technical field of resources and environments. The method comprises the following steps: culturing a carbonate mineralization strain by adopting radioactive soil, carrying out enlarged culture on the carbonate mineralization strain and montmorillonite with good impermeability, airtightness, cementation, curing performance and adsorption function, and centrifuging the materials to obtain a montmorillonite / carbonate mineralization bacteriumaggregate; then adding, in a batched manner, the montmorillonite / carbonate mineralized bacterium aggregate and the mixtureinto strontium-containing soil, and regulating the pH value of the soil and the nucleation effect of sodium chloride through hydrochloric acid in a hydrochloric acid sodium chloride solution, so that the strontium is mineralized, sealed in situ and solidified, and migration anddiffusion of strontium in the soil are stopped from the source.

Description

technical field [0001] The invention belongs to the technical field of resources and environment, and in particular relates to a method for treating strontium in soil by utilizing montmorillonite / carbonate mineralizing bacteria aggregates. Background technique [0002] With the continuous development of science and technology, the problem of environmental pollution is becoming more and more serious, seriously affecting people's health and quality of life. A series of activities such as the mining industry, national defense scientific research activities, radioactive waste disposal, and the opening of nuclear weapon test bases have led to increasingly serious problems of environmental radioactive pollution. Among them, the treatment and remediation of contaminated soil containing radionuclides has attracted increasing attention. [0003] At present, the methods for treating polluted soil include physical treatment, chemical treatment, engineering treatment and biological tre...

Claims

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Application Information

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IPC IPC(8): B09C1/08B09C1/10C12N1/20C12N11/14C12R1/07
CPCB09C1/08B09C1/10C12N1/20C12N11/14B09C2101/00
Inventor 竹文坤李显寅贠朝乐董云陈涛
Owner SOUTHWEAT UNIV OF SCI & TECH
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