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Preparation method and application of electrochemical sensor based on AuNPs@rGO@PS NSs

A sensor and electrochemical technology, applied in the field of preparation of electrochemical sensors, can solve the problems of high detection cost by two-phase electrophoresis, difficult to clearly distinguish phenotypes, unsuitable for large-scale research, etc., and achieves easy polymerization and uneven dispersion. , good catalytic activity, good electrical conductivity

Active Publication Date: 2020-09-29
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the isoelectric focusing method requires a large amount of serum, is expensive, time-consuming, requires large-scale equipment, and is not suitable for large-scale research
The difference in the degree of staining between apolipoprotein isomers and the variability of the swimming distance during electrophoresis also cause instability in the results; the detection cost of the two-phase electrophoresis method is relatively high, special equipment is required, and sometimes it is difficult to distinguish clearly phenotype; and electrochemical immunoassay has the advantages of high selectivity, fast response, simple operation, easy to carry and suitable for on-site detection, so researchers are actively exploring the development of new electrochemical sensors for the detection of apolipoprotein E4

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 A method for preparing an electrochemical sensor based on AuNPs@rGO@PS NSs, the steps are as follows:

[0041] (1) A glassy carbon electrode with a diameter of 3mm is covered with Al 2 o 3 The polishing powder is polished into a mirror surface and cleaned with ultra-pure water;

[0042] (2) 6 μL, 1.0 mg / mL Au-Ag / rGO@PDA dispersion liquid was drop-coated on the surface of the above electrode, dried at room temperature, rinsed with ultrapure water, and dried at room temperature;

[0043] (3) Continue to add 6 µL, 8 µg / mL of apolipoprotein E4 capture antibody Ab 1 Add dropwise to the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4 °C;

[0044] (4) Continue to add 3 µL, 0.5 mg / mL, 1% bovine serum albumin BSA solution dropwise to the electrode surface to seal the non-specific active sites on the electrode surface, rinse the electrode surface with ultrapure water, Remove unbound BSA and dry in a refrigerator at 4 °C;

[0045...

Embodiment 2

[0047] Example 2 A method for preparing an electrochemical sensor based on AuNPs@rGO@PS NSs, the steps are as follows:

[0048] (1) A glassy carbon electrode with a diameter of 4 mm is covered with Al 2 o 3 The polishing powder is polished into a mirror surface and cleaned with ultra-pure water;

[0049] (2) 6 μL, 2.0 mg / mL Au-Ag / rGO@PDA dispersion liquid was drop-coated on the surface of the above electrode, dried at room temperature, rinsed with ultrapure water, and dried at room temperature;

[0050] (3) Continue to add 6 µL, 10 µg / mL apolipoprotein capture antibody Ab 1 Add dropwise to the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4 °C;

[0051] (4) Continue to add 3 μL, 1 mg / mL, bovine serum albumin BSA solution with a mass fraction of 1% dropwise to the electrode surface to seal the non-specific active sites on the electrode surface, rinse the electrode surface with ultrapure water, Remove unbound BSA and dry in a refrigerator...

Embodiment 3

[0054] Example 3 A method for preparing an electrochemical sensor based on AuNPs@rGO@PS NSs, the steps are as follows:

[0055] (1) A glassy carbon electrode with a diameter of 5mm is covered with Al 2 o 3 The polishing powder is polished into a mirror surface and cleaned with ultra-pure water;

[0056] (2) 6 μL, 3.0 mg / mL Au-Ag / rGO@PDA dispersion solution was drop-coated on the surface of the above electrode, dried at room temperature, rinsed with ultrapure water, and dried at room temperature;

[0057] (3) Continue to add 6 µL, 8 ~ 12 µg / mL of apolipoprotein capture antibody Ab 1 Add dropwise to the surface of the electrode, rinse with ultrapure water, and dry in a refrigerator at 4 °C;

[0058] (4) Continue to add 3 µL, 1.5 mg / mL, bovine serum albumin BSA solution with a mass fraction of 1% dropwise to the electrode surface to seal the non-specific active sites on the electrode surface, rinse the electrode surface with ultrapure water, Remove unbound BSA and dry in a re...

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Abstract

The invention belongs to the technical field of immunoassay and biosensing, and provides a preparation method and application of an electrochemical sensor based on AuNPs@ rGO@PS NSs. Au-Ag / rGO@PDA isused as a substrate material, and the excellent conductivity, large specific surface area and high catalytic activity of Au-Ag / rGO@ PDA can effectively reduce background signals; meanwhile, AuNPs@rGO@PS NSs and detection antibody incubation are used as signal markers to further enhance the catalytic performance of the electrochemical sensor and amplify signals; with the method, quantitative detection of apolipoprotein E4 is realized, the preparation method has the advantages of low detection limit, high sensitivity, good repeatability, selectivity and stability and the like, and has importantscientific significance and application value for detection of apolipoprotein E4.

Description

[0001] the summary of instruction [0002] The invention belongs to the technical fields of immune analysis and biosensing, and provides a preparation method and application of an electrochemical sensor based on AuNPs@rGO@PS NSs. The present invention uses Au-Ag / rGO@PDA as the substrate material, its excellent electrical conductivity, large specific surface area and high catalytic activity can effectively reduce the background signal; at the same time, AuNPs@rGO@PS NSs and detection antibody incubation are used as signal markers In order to further enhance the catalytic performance of the electrochemical sensor and amplify the signal, the quantitative detection of apolipoprotein E4 has been realized, which has the advantages of low detection limit, high sensitivity, good repeatability, selectivity and stability, and the detection of apolipoprotein E4 Detection has important scientific significance and application value. technical field [0003] The invention belongs to the te...

Claims

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Application Information

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IPC IPC(8): G01N27/416G01N27/327G01N27/30G01N33/68G01N33/543
CPCG01N27/416G01N27/3278G01N27/308G01N33/6896G01N33/54306
Inventor 李月云谭明珅禹晓东王楠徐振
Owner SHANDONG UNIV OF TECH
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