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Method for improving electroporation cell transfection efficiency

A technology of transfection efficiency and electroporation, which can be applied to other methods of inserting foreign genetic materials, biochemical equipment and methods, electro/wave energy treatment enzymes, etc. The effect of reducing the partial pressure on the electrode surface, increasing the capacitance, and reducing the strength

Pending Publication Date: 2020-09-22
SHENZHEN CELLBRI BIO INNOVATION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there have been no relevant reports about optimizing the structure of the electroporation electrode of the electroporation device and the applied pulse voltage to improve its transfection rate and reduce its death rate

Method used

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  • Method for improving electroporation cell transfection efficiency
  • Method for improving electroporation cell transfection efficiency
  • Method for improving electroporation cell transfection efficiency

Examples

Experimental program
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Embodiment

[0031] The embodiment of the present invention provides a method for improving the transfection efficiency of electroporated cells, specifically, using an electroporation device with at least one electrode as a porous electrode to perform electroporation on the cell sample liquid to be treated.

[0032] Specifically, the core structure of the above-mentioned electroporation device is as follows: image 3 As shown, a fluid pool 2 is included, and the two ends of the fluid pool 2 are oppositely provided with a cell suspension inlet 3 and a cell suspension outlet 4. In addition, in the interior of the fluid pool 2, two electrodes are arranged in parallel. The sheet electrodes extend along the flow direction of the cell suspension in the fluid pool 2 , and at least one of the above two sheets of electrodes is a porous electrode 1 .

[0033] In detail, such as figure 1 Shown is the equivalent circuit network diagram of an electroporation device using a porous electrode as an elect...

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Abstract

The invention discloses a method for improving electroporation cell transfection efficiency. The method comprises the following steps: performing electroporation on cell sample liquid to be treated byadopting an electroporation device, wherein at least one of electrodes of the electroporation device is a porous electrode; and, performing electroporation on the cell sample liquid to be treated byapplying a bipolar pulse voltage to the electroporation electrode, wherein the pulse voltage of the bipolar pulse voltage is -2kV to +2kV. According to the invention, the porous electrode is used as the electroporation electrode, the electric double-layer capacitance of the electrode can be increased, the surface partial pressure of the electrode is reduced, the voltage applied to a solution is increased, the solution electrolysis and the cathode effect are relieved, and the cathode effect is a main factor for increasing the death rate of cells and reducing the transfection rate; and meanwhile, due to the fact that partial voltage on the electrodes is reduced, the intensity of applied voltage can be reduced under the same electric field intensity requirement.

Description

technical field [0001] The invention relates to the technical field of electroporation, in particular to a method for improving transfection efficiency of electroporated cells. Background technique [0002] Electroporation is a cell transfection method that generates a transmembrane voltage on the cell membrane by applying an external pulsed electric field. When the cell transmembrane voltage exceeds a certain threshold, recoverable micropores are formed on the cell membrane surface, thereby introducing foreign substances into cells. Electroporation will not cause permanent damage to cells, and the micropores formed by it can recover by itself. Electroporation makes it possible for biologically active substances to enter cells. [0003] In the process of electroporation, how to improve its transfection rate and reduce its death rate is a key technical problem that those skilled in the art urgently need to solve. So far, there have been no relevant reports about optimizing t...

Claims

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Application Information

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IPC IPC(8): C12N15/87C12N13/00
CPCC12N15/87C12N13/00
Inventor 郭霄亮商院芳石晓晓
Owner SHENZHEN CELLBRI BIO INNOVATION TECH CO LTD
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