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Preparation method of organophosphorus degrading enzyme-based multifunctional catalyst, organophosphorus degrading enzyme-based multifunctional catalyst and application of organophosphorus degrading enzyme-based multifunctional catalyst

A technology for degrading enzymes and organic phosphorus, which is applied in the preparation of organic compounds, catalyst activation/preparation, amino compound preparation, etc. It can solve the problems of poor stability and incomplete degradation products, and achieve simple operation, low enzyme purity requirements, good The effect of applying the effect

Active Publication Date: 2020-09-22
HEBEI UNIV OF TECH
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Problems solved by technology

[0005] In view of this, the present invention aims to propose a method for preparing an organophosphorus degrading enzyme-based multifunctional catalyst, so that the prepared organophosphorus degrading enzyme-based multifunctional catalyst can overcome the poor stability and degradation of organophosphorus pesticides in existing organophosphorus degrading enzymes. The problem of incomplete products

Method used

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  • Preparation method of organophosphorus degrading enzyme-based multifunctional catalyst, organophosphorus degrading enzyme-based multifunctional catalyst and application of organophosphorus degrading enzyme-based multifunctional catalyst
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  • Preparation method of organophosphorus degrading enzyme-based multifunctional catalyst, organophosphorus degrading enzyme-based multifunctional catalyst and application of organophosphorus degrading enzyme-based multifunctional catalyst

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Embodiment Construction

[0023] It should be noted that, in the case of no conflict, the embodiments of the present invention and the features in the embodiments can be combined with each other. At the same time, those who do not indicate specific conditions in the present invention can be carried out according to conventional conditions or the conditions suggested by the manufacturer of the equipment used, and the reagents or instruments used without indicating the manufacturer are all conventional products obtained by commercially available purchases. That is, if the technical means or process methods involved are not specified, they shall be carried out in accordance with the existing methods in the field.

[0024] The present invention will be described in detail below with reference to the accompanying drawings and examples.

[0025] This example relates to a preparation method of an organophosphorus degrading enzyme-based multifunctional catalyst. The composite egg yolk shell structure nanomater...

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Abstract

The invention provides a preparation method of an organophosphorus degrading enzyme-based multifunctional catalyst, the organophosphorus degrading enzyme-based multifunctional catalyst and applicationof the organophosphorus degrading enzyme-based multifunctional catalyst. The preparation method comprises the following steps: a composite yolk shell structure nano material is directly added into crude enzyme liquid of organophosphorus degrading enzyme with an affinity label, mixed and separated, and then the organophosphorus degrading enzyme-based multifunctional catalyst is obtained. The preparation method of the organophosphorus degrading enzyme-based multifunctional catalyst is easy to operate, the requirement for the enzyme purity is low, the carrier can be directionally combined with the enzyme, the prepared organophosphorus degrading enzyme-based multifunctional catalyst is low in cost, an organophosphorus pesticide can be detected, the organophosphorus pesticide can be further degraded in a cascade mode, and a final product, namely p-aminophenol has important application value.

Description

technical field [0001] The invention relates to the technical field of biocatalyst preparation, in particular to a method for preparing an organophosphorus degrading enzyme-based multifunctional catalyst. At the same time, the invention also relates to the organophosphorus degrading enzyme-based multifunctional catalyst prepared by the preparation method, and the application of the organophosphorus degrading enzyme-based multifunctional catalyst in the detection and degradation of organophosphorus pesticides. Background technique [0002] Organophosphorus pesticides are a kind of pesticides widely used in agricultural insecticide. There are a large number of organophosphorus compounds in organophosphorus pesticides. However, organophosphorus compounds are one of the most toxic substances known. It has caused a lot of pollution and caused serious harm to human health, so it is very important to detect and degrade it. [0003] In recent years, significant progress has been ma...

Claims

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Application Information

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IPC IPC(8): C12N11/14C12Q1/44C12Q1/34A62D3/02A62D101/04A62D101/26
CPCC12N11/14C12N9/16C12N9/14C12Q1/44C12Q1/34A62D3/02C12Y301/08001C07K2319/21C07K2319/20A62D2101/04A62D2101/26B01J23/755B01J31/003B01J2531/028B01J35/398B01J21/08B01J21/18B01J23/75B01J37/009B01J37/04C07C201/06C07C205/22C07C209/325B01J35/397
Inventor 姜艳军周丽亚薛赛光高静
Owner HEBEI UNIV OF TECH
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