Preparation method of allogeneic or xenogeneic tendon and bone tendon complex

Abstract

The invention relates to a preparation method of an allogeneic or xenogeneic tendon and bone tendon complex. The preparation method comprises the following steps that: S1: an allogeneic or xenogeneictendon and bone tendon complex material is obtained, and bloodstains on the surfaces of the tendons are cleaned with purified water; S2, supercritical CO2 extraction equipment is started, and the cleaned tendons are put into the supercritical CO2 extraction equipment for extraction; S3, the extracted tendons are subjected to decellularization treatment and grease washing; S4, the purified water isadded into the tendons subjected to oscillation washing, and oscillation washing is carried out in a shaking table at room temperature; S5, the tendons subjected to oscillation washing are trimmed, water on the surfaces of the trimmed tendons is drained off, and vacuum packaging is carried out under aseptic conditions; and S6, the packaged tendons with cobalt-60 under the dosage of 10-30 kGy areirradiated, and sterilization again is carried out so as to obtain the tendons subjected to vacuum packaging. The preparation method has the advantages of convenient processing, high efficiency, environmental protection and safety.

Description

technical field [0001] The invention relates to a preparation method of allogeneic or heterogeneous tendon and bone-tendon complex. Background technique [0002] Cruciate ligament injuries are common in sports injuries and traffic accident injuries. With the development of modern competitive sports and the increase in high-speed vehicle traffic accidents, the injuries of the cruciate ligament of the knee joint are also increasing year by year. The cruciate ligament is an important structure for the stability of the knee joint. After its injury, it can lead to joint pain and joint instability. In the later stage, it often causes joint laxity, joint degeneration, secondary meniscus injury and articular cartilage destruction. In severe cases, it can cause arthropathy waste. Therefore, the treatment after cruciate ligament injury has always been one of the important issues that orthopedic surgeons pay attention to. [0003] The accepted surgical method for knee cruciate ligame...

AI Technical Summary

Problems solved by technology

[0003] The accepted surgical method for knee cruciate ligament rupture is ligament reconstruction, and the most commonly used method is autologous material graft reconstruction, but at the expense of sacrificing the normal dynamic and stable functions of the existing knee joint, and lesions at the donor site may further increase the Joint dysfunction, and there are problems such as difficulty in tissue source during simultaneous reconstruction and revision of multiple ligament injuries: artificial ligament reconstruction technology is not mature enough to meet satisfactory histological and biomechanical requirements and long-term clinical efficacy ; Bioengineered ligaments are still in the research stage and cannot be applied clinically at present

However, neither the removal of antigens to reduce immunogenicity nor the effective virus inactivation of allogeneic or heterogeneous tendons can be done at the expense of mechanical properties of tendons

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