Application of luteolin in delaying quality deterioration of harvested fruits
A technology of luteolin and fruit, which is applied in the field of fresh-keeping of fruits and vegetables, can solve the problem of less luteolin, achieve the effect of improving sensory quality and delaying aging
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Embodiment 1
[0027] Prepare luteolin with water to a concentration of 100mg L -1 and 200mg L -1 The solution was evenly sprayed on the surface of the freshly harvested cherry fruit, only sprayed once, sprayed on the surface of the fruit evenly covered with foggy water droplets as a standard, while sterile water was used as a control. After drying, store under suitable conditions (25°C, relative humidity 85-90%), and test the sensory quality indicators such as weight loss rate, soluble solid content, titratable acid and natural incidence rate of samples for 6 consecutive days. The result is as figure 1 It was shown that luteolin can delay the change of fruit weight loss rate and soluble solid content, the content of titratable acid is always higher than that of the control group, and reduce the natural disease rate.
Embodiment 2
[0029] Prepare luteolin with water to a concentration of 100mg L -1 and 200mg L -1 The solution was evenly sprayed on the surface of the freshly harvested cherry fruit, only sprayed once, sprayed on the surface of the fruit evenly covered with foggy water droplets as a standard, while sterile water was used as a control. After drying, store under suitable conditions (25°C, relative humidity 85-90%), take samples every 2 days, store at -80°C, grind all samples, and measure phenylalanine ammonia lyase (PAL), cinnamon Acid-4-hydroxylase (C4H), 4-coumaric acid coenzyme A ligase (4CL) enzyme activity changes and corresponding gene (PAL, C4H, 4CL) expression levels. Such as figure 2 Shown, compare with contrast, illustrate that the present invention effectively activates fruit phenylalanine ammonia lyase (PAL), cinnamic acid-4-hydroxylase (C4H), 4-coumaric acid coenzyme A ligase ( 4CL) and increase the expression levels of PAL, C4H, and 4CL genes. We speculate that luteolin may ...
Embodiment 3
[0031] Prepare luteolin with water to a concentration of 100mg L -1 and 200mg L -1The solution was evenly sprayed on the surface of the freshly harvested cherry fruit, only sprayed once, sprayed on the surface of the fruit evenly covered with foggy water droplets as a standard, while sterile water was used as a control. After drying, store under suitable conditions (25°C, relative humidity 85-90%), take a sample every 2 days, and measure the total anthocyanin content and the expression level of corresponding genes (CHS, DFR, UFGT) for 6 consecutive days detection. Such as image 3 As shown, compared with the control group, the total anthocyanin content in the fruits of the treatment group was higher than that in the control group, and the gene expression levels of CHS, DFR, and UFGT were increased, indicating that luteolin can promote the synthesis of total anthocyanins.
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