Polypeptide monolayer film with 6% primary amino group exposure and its preparation method and application

A single-layer film and exposure technology, applied in the field of natural polymers, can solve the problems of limiting the application of polypeptide molecules, and achieve the effects of preventing postoperative infection, improving adhesion and proliferation, and reducing systemic side effects

Active Publication Date: 2022-01-25
QILU UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Due to the complexity of the structure of natural biological macromolecules, there are few reports on the surface properties of polypeptide monolayer membranes, which limits the application of polypeptide molecules.

Method used

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  • Polypeptide monolayer film with 6% primary amino group exposure and its preparation method and application
  • Polypeptide monolayer film with 6% primary amino group exposure and its preparation method and application
  • Polypeptide monolayer film with 6% primary amino group exposure and its preparation method and application

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Embodiment 1

[0054] A method for preparing a polypeptide monolayer film, comprising the following steps:

[0055] (1) Prepare 50 mL of collagen peptide solution with a concentration of 4% wt: accurately weigh 100 mL of collagen peptide in a three-necked flask, accurately measure deionized water, pour the deionized water into the three-necked flask, and swell at room temperature for 0.5 h. Put the three-neck flask in a water bath at 50±1°C, heat and stir for 2 hours to dissolve it completely, then adjust the pH of the solution to 10.00±0.02 with 2mol / L sodium hydroxide, and stabilize it in the water bath for 0.5 hours.

[0056] (2) Add surfactant SUS to the above-mentioned collagen polypeptide solution to obtain a collagen polypeptide-SUS mixed solution, the concentration of SUS in the mixed solution is 6.52mmol / L (at 50°C, the mass fraction of SUS in the mixed solution is 6%) ; Stable in a water bath for 6h for later use.

[0057] (3) Cut a rectangular titanium sheet with a size of 1cm×1c...

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Abstract

The invention provides a polypeptide monolayer film with a primary amino group exposure of 6%, as well as its preparation method and application. The polypeptide is composed of molecular weight (1.48 ± 0.2) × 10 5 g / mol of polypeptide molecules, the thickness of the monolayer film is 14.6±0.1nm, the exposure of primary amino groups on the surface of the film is 6±0.3%, and the Zeta potential of the polypeptide monolayer film is -4.48±0.1mV; the film The contact angle is 45±1°. The polypeptide monolayer film of the present invention has good stability, and the exposure amount of the primary amino group on the film surface is precisely controlled at 6.0 ± 0.3%, which can be applied to oral implant surface coating materials, can carry antibiotics and antibacterial drugs and is stable Release, the local drug-loaded coating of the polypeptide monolayer film has the advantages of low systemic side effects and strong antibacterial properties. It is evenly combined with pure titanium materials and is not easy to separate and fall off.

Description

technical field [0001] The invention belongs to the field of natural polymers, and relates to a polypeptide monolayer film and its preparation method and application, in particular to a polypeptide monolayer film with a primary amino group exposure of 6%, and its preparation method and application. Background technique [0002] Collagen peptide is a water-soluble protein obtained by chemical thermal degradation of collagen. It is one of the most commonly used biopolymers due to its excellent biocompatibility, plasticity, viscosity, abundance and low cost. As a biodegradable and renewable resource, collagen peptides are widely used in the preparation of medical materials, bionic materials, packaging and coating materials. Bio-immobilized coatings are often used in the field of biomimetic scaffolds to solve the problem of carrying biomolecules, drug molecules, synthetic polymers or small organic molecules such as enzymes, lactose, and polydopamine. The loading capacity is ea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/06A61L27/34A61L27/50A61L27/54
CPCA61L27/06A61L27/34A61L27/54A61L27/50A61L2430/12A61L2300/406A61L2300/404C08L89/00C08L79/02
Inventor 李天铎许静马慧君班青张震
Owner QILU UNIV OF TECH
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