Polypeptide monolayer film with primary amino group exposure of 7%, its preparation method and application

A single-layer membrane and primary amino group technology, applied in the field of natural polymers, can solve problems such as restricting the application of polypeptide molecules, and achieve the effects of preventing postoperative infection, less systemic toxic and side effects, and uniform and tight binding.

Active Publication Date: 2021-12-14
QILU UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Due to the complexity of the structure of natural biological macromolecules, there are few reports on the surface properties of polypeptide monolayer membranes, which limits the application of polypeptide molecules.

Method used

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  • Polypeptide monolayer film with primary amino group exposure of 7%, its preparation method and application
  • Polypeptide monolayer film with primary amino group exposure of 7%, its preparation method and application
  • Polypeptide monolayer film with primary amino group exposure of 7%, its preparation method and application

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Embodiment 1

[0054] A method for preparing a polypeptide monolayer film, comprising the following steps:

[0055] (1) Prepare 50 mL of collagen peptide solution with a concentration of 4% wt: accurately weigh 100 mL of collagen peptide in a three-necked flask, accurately measure deionized water, pour the deionized water into the three-necked flask, and swell at room temperature for 0.5 h. Put the three-neck flask in a water bath at 50±1°C, heat and stir for 2 hours to dissolve it completely, then adjust the pH of the solution to 10.00±0.02 with 2mol / L sodium hydroxide, and stabilize it in the water bath for 0.5 hours.

[0056] (2) Add surfactant SDSO to the above-mentioned collagen polypeptide solution to obtain a collagen polypeptide-SDSO mixed solution, the concentration of SDSO in the mixed solution is 8.04mmol / L (at 50°C, the mass fraction of SDSO in the mixed solution is 6%) ; Stable in a water bath for 6h for later use.

[0057] (3) Cut a rectangular titanium sheet with a size of 1c...

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Abstract

The invention provides a polypeptide monolayer film with a primary amino group exposure of 7%, its preparation method and application. The polypeptide is composed of molecular weight (1.48 ± 0.2) × 10 5 g / mol of polypeptide molecules, the thickness of the monolayer film is 8.9 ± 0.1nm, the exposure of primary amino groups on the surface of the film is 7.0 ± 0.2%, and the Zeta potential of the polypeptide monolayer film is 0.76 ± 0.1mV; The contact angle is 48±1°. The polypeptide monolayer film of the present invention has good stability, and the exposure amount of the primary amino group on the film surface is precisely controlled at 7.0 ± 0.2%, which can be applied to oral implant surface coating materials, can carry antibiotics and antibacterial drugs and is stable Release, the local drug-loaded coating of the polypeptide monolayer film has the advantages of low systemic side effects and strong antibacterial properties. It is evenly combined with pure titanium materials and is not easy to separate and fall off.

Description

technical field [0001] The invention belongs to the field of natural polymers and relates to a polypeptide monolayer film and its preparation method and application, in particular to a polypeptide monolayer film with a primary amino group exposure of 7% and its preparation method and application. Background technique [0002] Collagen peptide is a water-soluble protein obtained by chemical thermal degradation of collagen. It is one of the most commonly used biopolymers due to its excellent biocompatibility, plasticity, viscosity, abundance and low cost. As a biodegradable and renewable resource, collagen peptides are widely used in the preparation of medical materials, bionic materials, packaging and coating materials. Drug molecules; the loading of synthetic polymers or small organic molecules, if the collagen peptide is prepared into a polypeptide monolayer membrane, it has the advantages of easy and precise control of the loading amount. [0003] However, the thickness ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/06A61L27/34A61L27/50A61L27/54
CPCA61L27/06A61L27/34A61L27/54A61L27/50A61L2430/12A61L2300/406A61L2300/404C08L89/00C08L79/02
Inventor 许静李天铎班青马慧君张震
Owner QILU UNIV OF TECH
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