Construction method of sarcopenia- osteoporosis rat model

Abstract

The invention discloses a construction method of a sarcopenia-osteoporosis rat model. The method comprises the following steps of selecting female SD rats which are not pregnant and 6 months old, removing bilateral ovaries, feeding for 1 week, injecting 0.8-1.2 mg / kg / d of dexamethasone sodium phosphate injection into the abdominal cavities of the rats, and continuously injecting for 2 weeks; continuously feeding the rats for 8-9 weeks to obtain a rat model with sarcopenia-osteoporosis; meanwhile, a simple and reliable sarcopenia-osteoporosis judgment index is also determined. The composite modeling method has the advantages of being short in modeling period, stable in model, high in rat survival rate, high in operability, low in cost and the like, SP and OP can be effectively distinguished, sarcopenia-osteoporosis of human beings can be truly simulated, repeatability is high, economy and practicability are achieved, and a theoretical basis is provided for researching etiology and pathogenesis and prevention and treatment strategies of OS.

Description

technical field [0001] The invention relates to the field of biological model construction, in particular to a method for constructing a sarcopenia-osteoporosis rat model. Background technique [0002] Osteoporosis (OP) is a disease of the skeletal system characterized by decreased bone mass, bone microarchitectural damage, and increased bone fragility. Sarcopenia (SP) or sarcopenia is a decrease in muscle strength, muscle mass, or muscle function. Disorders of the muscular system. When OP and SP coexist, we call it sarcopenia-osteoporosis (OS), that is, OS has the clinical features of OP and SP at the same time, and its pathogenesis is far more complicated than that of sarcopenia or osteoporosis alone , patients have a high risk of falls, fragility fractures, and mortality, and are more likely to have other comorbidities. Moreover, the interaction of fragility fractures (such as hip joint fractures, lumbar vertebral fractures, and distal radius fractures, etc.) caused by ...

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Problems solved by technology

Among them, DXM is a hormonal drug with the highest potency and the longest duration of action among glucocorticoids. Using it to establish SP animal models has the advantages of a short cycle and obvious effects, but DXM belongs to glucocorticoids and has anti-inflammatory properties. , anti-allergic and anti-shock effects, long-term injections can cause side effects such as weight gain, muscle atrophy, and fat accumulation

In addition to injecting DXM, SP animal models can also be constructed by feeding rats with high-fat diets; however, this method generally has problems such as long modeling period (up to 14 weeks), high feeding costs, and inconsistent high-fat diet formulations, making it difficult to be widely used

[0004] However, compared with OP or SP, the current research on OS is only in its infancy, limited to clinical observation, and there is no report on the method of constructing an animal model of OS.

Some scholars tried to use 10-month-old female SD rats to ovariectomize the OS model, but this method has problems such as long animal feeding period, old rats are prone to death, and evaluation indicators are one-sided, and cannot effectively distinguish SP and OP.

Moreover, there are still controversies on the diagnostic criteria of OS at home and abroad. There is no uniform clinical diagnostic criteria in the world. The diagnosis of the disease must meet the diagnostic criteria of OP and SP at the same time. This is crucial for the establishment of OS animal models, as well as the clinical diagnosis, Basic research, etc. bring great difficulties

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