Method for constructing urinary calculus animal model by using locust
A technology of urinary stones and animal models, applied in the field of biomedical research, can solve problems such as obstacles and restrictions on effective medical treatment, and achieve high efficiency, economic convenience, and good test repeatability
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Embodiment 1
[0023] 1. Catch O. sinensis adults from the paddy field, put them into a ventilated sarong and then place them in a climate incubator and feed them adaptively with fresh wheat seedlings for 2-3 days. The optimal feeding environment conditions are: the light and dark time is 13:11 hours , the temperature in light is 25±2℃, the temperature in darkness is 21±1℃, and the humidity is 60-65%;
[0024] 2. Precisely configure the formula of standard insect saline: NaCl: 0.138 M, KCl: 0.0027 M, NaCl: 0.0027 M, NaCl 2 HPO 4 : 0.0073M, KH 2 PO 4 : 0.00147 M, pH=7.4; the prepared insect saline was placed in a Gaoyan sterilizer and sterilized by steam at 120°C for 20 minutes;
[0025] 3. Prepare a 10.5 mM copper chloride solution with the above-mentioned sterilized insect saline;
[0026] 4. Randomly select the rice locusts that have been adaptively reared, spray 75% alcohol on their body surface for disinfection, and use a micro-syringe to draw 75% alcohol to rinse the inner tube of t...
Embodiment 2
[0034] Example 2: In this example, the concentration of the copper chloride solution injected into the abdominal cavity of Oryza chinensis is 7.0 mM, and the injection volume is 5 μL. 2°C, the temperature in the dark is 20±2°C, the humidity is 70-75%, and other operations are the same as in Example 1.
Embodiment 3
[0035] Example 3: In this example, the concentration of the copper chloride solution injected into the abdominal cavity of Oryza chinensis was 8.0 mM, and the injection volume was 6 μL. The feeding conditions and other operations of Oryza chinensis were the same as in Example 1.
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