W/O/W type unsaturated guluronic acid nanometer cream and preparation method thereof
A guluronic acid, unsaturated technology, applied in oil/fat/wax inactive ingredients, pharmaceutical formulations, emulsion delivery, etc., can solve the problems of unstable storage process, poor water solubility, etc., to protect antioxidant activity Effect
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Embodiment 1
[0038] Example 1 Preparation of unsaturated guluronic acid and identification of degree of polymerization
[0039] Sodium alginate was dissolved in 0.5M HCl, heated at 90°C for 7h and then allowed to stand overnight to separate layers. Then centrifuge at 3500rpm at a low speed for 10min to obtain a precipitate for later use. Precipitate with 8% NaHCO 3 After the solution was dissolved, the pH was adjusted to 2.85 with HCl, the layers were allowed to stand, and after centrifugation at a low speed of 3500 rpm for 10 min, the precipitate was collected, dissolved in water, and lyophilized to prepare PG. Weigh a certain amount of PG and dissolve it in PBS. Add a certain amount of alginate lyase, react at a constant temperature at 37°C for 2h, then add the same amount of enzyme solution, and continue to react at 37°C for 24h. After the reaction was completed, heat in a water bath at 80°C for 10 minutes to inactivate the enzyme, filter through a 0.45 μm microporous membrane after ...
Embodiment 2
[0041] Embodiment 2 prepares W / O / W type unsaturated guluronic acid nanoemulsion
[0042] Dissolve unsaturated guluronic acid in water as the inner aqueous phase (W 1 Mutually);
[0043] Mix 15% polyglycerol ricinoleate and 85% edible oil evenly as the oil phase (O phase);
[0044] Mix 10% of hydrophilic emulsifier (Span-80 / Tween-80 compound, HLB value is 10), 1% of polyvinyl alcohol and water as the external water phase (W 2 Mutually);
[0045] W 1 Phase and O phase were mixed at a mass ratio of 3:7, and after being homogenized by a high-speed disperser (14000rpm, 2min), the W 1 / O colostrum;
[0046] Combine colostrum with W 2 The phases are mixed according to the mass ratio of 3:7, and homogenized by a high-speed disperser (14000rpm, 2min) to obtain a double emulsion, which is the W / O / W type unsaturated guluronic acid nanoemulsion provided by the present invention.
[0047] On the basis of single factor investigation, it is determined that linseed oil is used as the o...
Embodiment 3
[0056] Example 3 Particle Size Detection and Electron Microscopic Observation of Unsaturated Guluronic Acid Nanoemulsion
[0057] After the unsaturated guluronic acid nanoemulsion in embodiment 2 is diluted 10 times with ultrapure water, drop on the copper grid that is covered with support film, evaporate to dry naturally, observe nanoemulsion form with transmission electron microscope, see appendix image 3 . The approximately circular black dots in the figure are unsaturated guluronic acid nanoemulsion droplets with uniform size.
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