Rhizopus oryzae variant NC-6 for producing L-lactic acid with high yield and high optical purity and application of Rhizopus oryzae variant NC-6

Abstract

The invention discloses a Rhizopus oryzae variant NC-6 for producing L-lactic acid with high yield and high optical purity. The Rhizopus oryzae variant NC-6 was preserved in China General Microbiological Culture Collection Center (CGMCC) on April 20th, 2020, the preservation address is No. 3, No.1 Yard, Beichenxi Road, Chaoyang District, Beijing City, the Rhizopus oryzae variant NC-6 is classifiedand named as Rhizopus oryzae, and the preservation number is CGMCC NO. 19620. The high-yield strain for producing the L-lactic acid with high yield and high optical purity is specifically identifiedas a new Rhizopus oryzae variant by performing morphological analysis, ITS rDNA sequence analysis, whole genome re-sequencing analysis and comparative analysis on sequence similarity Blast of proteincoding genes actin, EF-1 alpha (translation elongation factor 1-alpha) and RPB2 (the largest subunit of the RNA polymerase II). The strain is cultured by shake flask fermentation, and it discovers that the L-lactic acid yield of the strain reaches 78.00 g / L, the sugar conversion rate reaches 73.58%, and the optical purity is 100%; and the strain is cultured by amplification in a 200 L fermentationtank, the L-lactic acid yield of the strain reaches 86.07 g / L, the sugar conversion rate is 83.21%, the optical purity is 100%, and the yield is stable. The strain has wide industrial application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a microbial variety and its application, in particular to Rhizopus oryzae variety NC-6 with high yield and high optical purity L-lactic acid and its application. Background technique [0002] In 1780, Swedish scientists first discovered lactic acid, also known as α-hydroxypropionic acid (or 2-hydroxypropionic acid). Due to the asymmetry of its molecular structure, lactic acid is the smallest chiral molecule existing in nature. Lactic acid can be classified into L-type D-lactic acid, D-type L-lactic acid and racemic DL-lactic acid according to its optical activity. Lactic acid is not only a metabolite that exists widely in the biological world, but also an important medicinal chemical intermediate, which can be used to prepare chemicals or biological materials such as alkyl lactate, propylene glycol, propylene oxide, acrylic acid, and polylactic acid. Due to the advantages of readily ...

AI Technical Summary

Problems solved by technology

At present, the preparation of L-lactic acid is relatively complicated and costly, and the preparation technology or method of L-lactic acid has become a bottleneck affecting the development of lactic acid-related industries. Therefore, research or develop a low-cost, efficient and convenient production or preparation of L-lactic acid Technology has become a major topic in this field, and the search for high-yield, high-optical-purity L-lactic acid-producing strains has become a very meaningful and valuable research direction

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