Kit for simultaneously detecting multiple antipsychotic drugs in serum

Abstract

The invention discloses a kit for simultaneously detecting multiple antipsychotic drugs in serum. An LC-MS / MS method is adopted; 16 common antipsychotic drugs can be detected at one time; the kit is simple in pretreatment process, low in cost, high in sensitivity and high in specificity; separation and detection of the antipsychotic drug are completed within 5 min, accuracy and precision basicallymeet the requirements, the kit can be used for quantitative analysis of the clinical antipsychotic drug, and a reliable detection method is provided for monitoring the treatment concentration of theclinical antipsychotic drug.

Description

technical field [0001] The invention belongs to the technical field of drug analysis, in particular to a kit for simultaneously detecting multiple antipsychotic drugs in serum. Background technique [0002] Antipsychotics are drugs indicated for the treatment of a variety of mental disorders, including schizophrenia, hallucinations, mania, sleep disturbances, and dementia. There are 9 categories and more than 40 kinds of antipsychotic drugs that have been used clinically, among which the classic antipsychotic drugs include phenothiazines, thioxanthenes, butyrophenones, benzamides and diphenoxazepines. However, all antipsychotic drugs can cause unpleasant side effects and severe intoxication after overdose, and these problems may be increased when antipsychotic drugs are used in combination. Unpleasant side effects often increase nonadherence in psychiatric patients. Therefore, suitable analytical methods are necessary for the screening, identification and quantification of ...

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for detecting the concentration of antipsychotic drugs in vivo mainly include high-performance liquid chromatography and ultra-high performance liquid chromatography-tandem mass spectrometry. "mass spectrometry with atmospheric pressure chemical ionization" reported a method for detecting 15 psychotropic drugs and some metabolites in plasma, but the sample volume is large, requiring 0.5mL, and SPE is required before, the cost is high, and one needle collection takes 10 minutes, linear Narrow range, etc.; the literature "Therapeutic drug monitoring of seven psychotropic drugs and four metabolites in human plasma by HPLC-MS" reports a method for the detection of multiple psychotropic drugs and metabolites in plasma, which has complex pretreatment, long sampling time, narrow linear range, etc.

Patent CN109668979A discloses a method for simultaneous detection of 17 kinds of antipsychotic drugs in blood samples. This method has a large injection volume, 20 μL is required for one needle injection, and the pretreatment is complicated. It needs nitrogen blowing to concentrate and redissolve, and it needs to be filtered. The cost is high. The collection time is long, it takes 9 minutes

Patent CN110455945A discloses a method and kit for detecting 5 kinds of psychotropic drugs and their main metabolites in blood. This method requires a large sample size of 100 μL, and can only detect 5 kinds of drugs at a time, which cannot meet the requirements of high-throughput drug screening. check request

In addition, there are existing technologies that detect one or several of these drugs, and the efficiency is relatively low, and there are deficiencies such as long analysis time for a single sample, low sensitivity, unsuitable linear range, complex sample pretreatment, and matrix effect interference.

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