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A method of two-step inoculation to increase fruiting bodies of Cordyceps militaris

A technology for Cordyceps militaris and grass seeds is applied in the field of Cordyceps militaris fruiting body biological yield, and can solve the problems that it is difficult to judge the mixed performance of two mating types, the ability of bacteria to decompose the culture material, and the biological yield, etc. Physical yield, reduced effects of bacterial degradation, high biological yield

Active Publication Date: 2021-11-26
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] 3. Degeneration of strains caused by factors such as malnutrition, manifested as reduced ability of strains to decompose compost and reduced biological output
From the perspective of karyotype monitoring, for a strain culture (incline or liquid bacterial liquid), although it can be determined from the single hyphae level whether it is a single mating type, it is difficult to judge whether the two mating types are from the population level of the strain. Is the heterokaryon or a mixed expression of two homokaryons that have decomposed

Method used

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  • A method of two-step inoculation to increase fruiting bodies of Cordyceps militaris
  • A method of two-step inoculation to increase fruiting bodies of Cordyceps militaris
  • A method of two-step inoculation to increase fruiting bodies of Cordyceps militaris

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) Inoculate the Cordyceps militaris monokaryon strain to the Cordyceps militaris cultivation medium according to the conventional method

[0036] The liquid strain of Cordyceps militaris monokaryon was prepared by a conventional method, inoculated in the culture medium of Cordyceps militaris, and cultured until the third day through conventional culture processes such as dark culture and light culture respectively. Conventional culture method and monokaryon strain obtaining method are quite mature in this field, and those skilled in the art can implement, and can make improvement in various degrees, and concrete operation method is referred to in the present embodiment (Gao Xinhua 2008, pupae Research on the mating type of Cordyceps militaris, Acta Edible Fungi Sinica 15(1): 1-5., see section 1.3 for details). The cultivation medium formula used in this example is: each bottle contains 20 grams of wheat kernels, 30 milliliters of water, used after autoclaving, and 50 ...

Embodiment 2

[0042] (1) Inoculate the Cordyceps militaris monokaryon strain to the Cordyceps militaris cultivation medium according to the conventional method

[0043] The liquid strain of Cordyceps militaris monokaryon was prepared by a conventional method, inoculated in the cultivation medium of Cordyceps militaris, and subjected to conventional culture processes such as dark culture and light culture respectively, and cultivated until the 8th day.

[0044] (2) Inoculate another Cordyceps militaris monokaryotic strain containing the opposite mating type gene from the monokaryotic strain inoculated in the first step to the Cordyceps militaris culture after inoculating in the first step according to a conventional method.

[0045] After the two-step inoculation, cultivate according to the conventional culture method of Cordyceps militaris and manage the fruiting body until the fruiting body matures, then harvest and measure the fresh weight and dry weight of the fruiting body. (other steps...

Embodiment 3

[0049] (1) Inoculate the Cordyceps militaris monokaryon strain to the Cordyceps militaris cultivation medium according to the conventional method

[0050] The liquid strain of Cordyceps militaris monokaryon was prepared by a conventional method, inoculated in the culture medium of Cordyceps militaris, and subjected to conventional culture processes such as dark culture and light culture, and cultivated until the 15th day.

[0051] (2) Inoculate another Cordyceps militaris monokaryotic strain containing the opposite mating type gene from the monokaryotic strain inoculated in the first step to the Cordyceps militaris culture after inoculating in the first step according to a conventional method.

[0052] After the two-step inoculation, cultivate according to the conventional culture method of Cordyceps militaris and manage the fruiting body until the fruiting body matures, then harvest and measure the fresh weight and dry weight of the fruiting body. (other steps are with embodi...

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Abstract

The invention discloses a two-step inoculation method for increasing the fruiting body yield of Cordyceps militaris. The method comprises first inoculating the monokaryotic strain of Cordyceps militaris on the culture medium of Cordyceps militaris and cultivating it for a period of time; Inoculate the Cordyceps militaris monokaryon, heterokaryon or homokaryon strains of the opposite mating type gene of the inoculated monokaryotic strain to the culture of Cordyceps militaris inoculated for the first time, and continue to cultivate until the fruiting body of Cordyceps militaris is harvested . The two-step inoculation method adopted in the present invention can not only reduce the impact of strain degeneration caused by karyotype changes in large-scale production, but also greatly improve the biotransformation rate of the fruiting body of Cordyceps militaris, so the biological yield of the fruiting body of Cordyceps militaris can be effectively improved, The invention is an important innovation in the field of edible fungus strain inoculation.

Description

technical field [0001] The invention relates to the technical field of edible fungus cultivation, in particular to a method for increasing the biological yield of fruiting bodies of Cordyceps militaris by artificially regulating the inoculation timing and mode of Cordyceps militaris strains. Background technique [0002] Cordyceps militaris (Cordyceps militaris), also known as Cordyceps militaris, Cordyceps militaris, belongs to Ascomycotina, Sclerotinia, Ergotomycetes, Ergotaceae, Cordyceps genus in fungal taxonomy, and is the model of fungi of the genus Cordyceps kind. Cordyceps fungi are a general term for a class of insect fungus complexes that are parasitic on the underground fruiting bodies of insects, spiders or plants of the genus Macrocystis. Cordyceps militaris mainly hosts the larvae and pupae of some insects of Lepidoptera, Coleoptera and Diptera in the wild. Cordyceps militaris not only contains a large amount of protein, vitamins and other nutrients, but also...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/00A01G18/50A01G18/40A01G18/20
CPCA01G18/00A01G18/20A01G18/40A01G18/50
Inventor 高新华孙丽娜黄开华陈伟
Owner SHANGHAI ACAD OF AGRI SCI
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