Bacillus gorilla yc9l and its application

A Bacillus and gorilla technology, applied in the field of microbial resources, can solve the problem that the functional genes of active substances have not been excavated and studied, and achieve the effects of improving the germination rate, promoting growth, and promoting plant growth.

Active Publication Date: 2021-11-19
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a new strain resource, its active substances and functional genes have not been excavated and studied

Method used

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  • Bacillus gorilla yc9l and its application
  • Bacillus gorilla yc9l and its application
  • Bacillus gorilla yc9l and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Screening of YC9L bacterial strain

[0038] (1) Separation

[0039] Taking the rhizosphere soil of the vegetation in the Tianhe Green Belt in Guangzhou as the screening soil sample, weigh 10g of the soil sample and put it into a 250mL triangular flask (with glass beads) filled with 90mL of sterile water, and shake it on a shaker for 30min. Fully disperse the bacteria and let it stand for 20-30s. Then in a 90°C water bath for 10 min, the supernatant was taken for 10-fold incremental dilution, using 10 -3 -10 -4 For dilution, pipette 100 μL of each gradient and spread on LB solid plate, and incubate upside down at 37°C for 24 hours. Observe and according to the growth characteristics of the colony shape, size, color, etc., pick the colonies and purify them on the LB solid medium, and then save the isolated bacteria for future use.

[0040] (2) screening

[0041] The ability of the isolated strains to produce IAA was determined by the Salkowski colorimetric ...

Embodiment 2

[0042] Embodiment 2 YC9L bacteria belong to Gram-positive bacteria

[0043] Pick a single colony of YC9L and culture it in liquid LB medium for 24 hours, pipette 4 μL of sterile water onto a glass slide, pipette 1 μL of bacterial liquid into the added sterile water, mix well, and then dry naturally. Fix with flame 2-3 times; first dye with crystal violet for 1 min, wash with water, and dry naturally; mordant with iodine solution for 1 min, wash with water, and dry; decolorize with 95% ethanol for 20 seconds, wash with water, and dry; counterstain with safranin for 1 min, wash with water , dried, and observed under a 100-fold oil immersion microscope, the thalline was blue-purple ( figure 2 A), YC9L is a Gram-positive bacterium.

Embodiment 3

[0044] Example 3 YC9L bacteria can produce spores

[0045] Pick a single colony of YC9L and culture it in liquid LB medium for 24 hours, pipette 4 μL of sterile water onto a glass slide, pipette 1 μL of bacterial liquid into the added sterile water, mix well, and then dry naturally. Pass the flame 2-3 times to fix. Add malachite green dye solution to the fixed smear, clamp the glass slide with tweezers and heat it on the flame of alcohol lamp, so that the dye solution will steam for 3-4 times within 30 seconds; wash with water and dry; reconstitute with safranin aqueous solution Dye for 1min, wash with water, and dry. Carry out microscopic examination with 100 times oil lens, spore is in green, and thalline is in red ( figure 2 B), YC9L bacteria can produce spores.

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Abstract

The invention discloses a gorilla bacillus (B.massiliogorillae) YC9L and its application; the strain preservation number is GDMCC No: 60979, and is preserved in the Guangdong Microbial Culture Collection Center; the YC9L strain has the properties of producing IAA, dissolving phosphorus and potassium and The repair function of heavy metal cadmium, zinc and copper stress, has the safety of negative hemolysis, can effectively promote seed germination, and promote the growth of lettuce and corn plants; the strain has fast propagation speed, simple production process, strong stress resistance, and easy preservation. It has broad application prospects in promoting plant growth, improving soil nutrients, and repairing heavy metals. It provides a new type of rhizosphere growth-promoting bacterial strain resource for microbial fertilizers; it belongs to the field of microbial resources.

Description

technical field [0001] The invention relates to a strain of Bacillus gorilla (B. massiliogorillae) YC9L and the use of Bacillus gorilla (B. massiliogorillae) YC9L, belonging to the field of microbial resources. Background technique [0002] Plant growth promoting rhizobacteria (PGPR) refers to a class of beneficial bacteria that live in the soil or epiphyte on the root system, which can promote plant growth and the absorption and utilization of mineral nutrients, and can inhibit harmful organisms. . PGPR secretes indole-3-acetic acid (IAA), which has the effect of increasing seed germination rate and root growth, and promoting plant growth and development. Studies have shown that the number of stem branches of Brassica napus was significantly increased after the roots of Brassica napus were inoculated with PGPR capable of producing IAA (Asghar, 2004). Hoffman et al. (2013) isolated a fungus with high yield of IAA from endophytic fungi in plants, and soaked the seeds with t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05G3/00B09C1/10C12R1/07
CPCB09C1/10C05G3/00C12N1/20C12N1/205C12R2001/07
Inventor 蔡燕飞李福艳王玉琪章家恩颜静婷乔凯
Owner SOUTH CHINA AGRI UNIV
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