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A Lecanococcus spp. capable of inhibiting Fusarium pathogenic bacteria and its application

A technology of Cyclocystis serrata and Fusarium spp. is applied in the fields of microorganisms and agriculture, and can solve the problems of no development of biological drug root rot, environmental pollution, drug residues, etc. The effect of bacteria-plant symbiosis

Active Publication Date: 2021-02-19
GANSU ACAD OF SCI INST OF BIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the prevention and treatment of plant root rot and pitting mainly depends on chemical drugs, such as watering the roots of plants with chemical drugs such as copper ammonia, metalaxyl, carbendazim, acetoallin and copper humate to prevent root rot The occurrence of diseases, but the above-mentioned drugs will cause environmental pollution and cause drug residues
Biological control is the focus of research at this stage, but those skilled in the art have not developed effective biological drugs for the prevention and treatment of root rot caused by Fusarium pathogenic bacteria. For the above technical problems, the present invention aims to find a method that can effectively Biopharmaceuticals for controlling plant root rot

Method used

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  • A Lecanococcus spp. capable of inhibiting Fusarium pathogenic bacteria and its application
  • A Lecanococcus spp. capable of inhibiting Fusarium pathogenic bacteria and its application
  • A Lecanococcus spp. capable of inhibiting Fusarium pathogenic bacteria and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment one bacterial strain is isolated

[0035] Rinse the excavated healthy Gansu Fritillaria and Angelica sinensis with running water for 10 minutes, blot the surface moisture with filter paper, cut off the roots of Fritillaria and Angelica sinensis, rinse 3-4 times with sterile distilled water, 75% Soak in ethanol for 30s, then rinse with sterile water for 2 to 3 times, then sterilize with 0.2% mercuric chloride for 10 minutes, then rinse with sterile water for 3 to 4 times, and use 100 μl of sterile water after the final rinse to smear the plate on Tiger Red Solid medium served as a control.

[0036] Use sterilized filter paper to dry the surface moisture of Fritillaria and Angelica explants after surface disinfection, cut the explants into about 1 cm with a sterilized knife, take 1 g and place them in a high-temperature dry-heat sterilized container at 200 °C. In the mortar, add 10ml of sterilized physiological saline, and grind thoroughly.

[0037] The stock...

Embodiment 2

[0039] Example two strain identification

[0040] Inoculate the different bacterial strains obtained in Example 1 into liquid PDA medium, culture them at 28°C and 200rpm for 7-10 days, collect the bacteria by centrifugation at 4°C and 5000rpm, and extract the genomic DNA with the SDS method after the tissue disruptor is fully broken , used for PCR amplification after electrophoresis detection. Primers are:

[0041] ITS1: 5'-TCCGTAGGTGAACCTGCGG-3'

[0042] ITS4: 5'-TCCTCCGCTTATTGATATGC-3'

[0043] The PCR reaction system is 50μl: 5× Buffer (Mg 2+ plus) 10μl, forward and reverse primers 2μl each, dNTP Mixture (each 2.5mM) 4μl, HS DNA Polymerase (2.5Μ) 0.5μl, DNA template 1μl, ddH 2 O 31.5 μl.

[0044] PCR amplification conditions: denaturation at 98°C for 10 sec; annealing at 56°C for 15 sec, extension at 72°C for 1 min and 30 sec, 30 cycles, and extension at 72°C for 5 min. The amplified products were sent to Beijing Qingke Xinye Biotechnology Co., Ltd. for sequencing...

Embodiment 3

[0047] Example 3 Qualitative Detection of Nitrogen Fixation Activity of Lecanococcus spp. BMDBLJJ-1

[0048] Prepare Ashubei culture medium, add 0.5% Congo red 5ml / L, sterilize and make a plate for use, pick a small amount of mycelia from the edge of the preserved Lecanococcus sp. culture medium at 28°C in the dark for 10 days, and observe the growth of the colonies.

[0049] Congo red is a brownish-red powder, which is yellow-red when dissolved in water and orange when dissolved in alcohol. It is used as an acid-base indicator with a color change range of 3.5 to 5.2. It is red in the alkaline state and blue-purple in the acid state. Nitrogen fixation by bacteria BMDBLJJ-1 is in an alkaline state, shown as a red circle. The size of the circle and the depth of the color can reflect the strength of nitrogen fixation ability to a certain extent.

[0050] Experimental results such as Figure 6 As shown, the colonies on the nitrogen-free ashibe culture medium were clearly visibl...

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Abstract

The invention relates to the technical fields of microbes and agriculture, in particular to a lecanococci strain capable of inhibiting pathogenic bacteria of the genus Fusarium and its application. The said Lecanococcus spp. BMDBLJJ-1 was preserved in the China General Microorganism Culture Collection Management Center on October 11, 2019, and the preservation number is CGMCC No.18585. The said Lecanococcus spp. BMDBLJJ-1 can Inhibit the pathogenic bacteria of the genus Fusarium, significantly reduce the incidence of root rot and pitting disease of Fritillaria and Angelica caused by the infection of pathogenic bacteria of the genus Fusarium, increase the rate of greening of the Fritillaria, and can be used to prevent and treat diseases caused by pathogenic bacteria of the genus Fusarium Various plant diseases.

Description

technical field [0001] The invention relates to the technical fields of microbes and agriculture, in particular to a Lecanococcus spp. which can inhibit the pathogenic bacteria of the genus Fusarium and its application. Background technique [0002] Fusarium Link exFr. (Fusarium Link exFr.), also known as Fusarium Link exFr., in taxonomy, the asexual period of Fusarium Link exFr. originally belonged to the subphylum Deuteromycetes, Tumorellales. The sexual period is Ascomycota, and the sexual state is often Gibberella. Fusarium is a worldwide distribution of fungi, it can not only survive winter and summer in the soil, Fusarium can also infect a variety of economic crops, causing plant root rot, stem rot, stem base rot, flower rot and ear rot The main diseases are rot disease, fusarium wilt and pitting disease, etc. There are many kinds of host plants, including medicinal plants, economic crops, etc., which mainly cause wilting and death of crops, affecting yield and qualit...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/20A01P3/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 杨涛王治业赵疆马永贵谢惠春杨晖巩晓芳方彦昊赵志敏
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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