Magnetic composite nano material and preparation method and application thereof

A technology of magnetic composite nano and magnetic nanoparticles, which is applied in the fields of nanomaterials and biomedicine, and can solve problems affecting the uptake of nanoparticles by cells

Active Publication Date: 2020-07-10
CIXI INST OF BIOMEDICAL ENG NINGBO INST OF MATERIALS TECH & ENG CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is known that polyethylene glycol as a surface modification of nanoparticles can prolong the in vivo circulation time of nanoparticles, but also affects the uptake of nanoparticles by cells

Method used

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  • Magnetic composite nano material and preparation method and application thereof
  • Magnetic composite nano material and preparation method and application thereof
  • Magnetic composite nano material and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0117] A preparation method of a pH-responsive magnetic composite nanoparticle diagnosis and treatment reagent comprises:

[0118] Step 1: Dissolve 8g of methoxy PEG (mPEG) and 1.8g of p-formylbenzoic acid (FBA) in 160mL of dichloromethane, add 20mL of dimethylformamide (DMF), and then add 2.48g of dicyclohexyl Carbodiimide (DCC) and 732mg 4-dimethylaminopyridine (DMAP), stirred for 24h.

[0119] Step 2: The system obtained in step 1 was subjected to vacuum filtration for 3 times to remove the precipitate, and the obtained clarified system was purged with nitrogen to remove the solvent, then dispersed in 50 mL of deionized water, and freeze-dried to obtain mPEG-FBA (a polymer with an aldehyde phenyl group at the end). )powder.

[0120] Step 3: Synthesis of superparamagnetic ferric oxide nanoparticles (SPION): get 0.81g ferric chloride hexahydrate (FeCl 3 ·6H 2 O), 0.556g ferrous sulfate heptahydrate (FeSO 4 ·7H 2 O), dissolved in 15mL of deionized water, after the reactio...

Embodiment 2

[0130] Step 1: Dissolve 8g of methoxy PEG (mPEG) and 1.8g of p-formylbenzoic acid (FBA) in 160mL of dichloromethane, add 20mL of dimethylformamide (DMF), and then add 2.48g of dicyclohexyl Carbodiimide (DCC) and 732mg 4-dimethylaminopyridine (DMAP), stirred for 24h.

[0131] Step 2: The system obtained in step 1 was vacuum filtered three times to remove the precipitate, and the obtained clarified system was purged with nitrogen to remove the solvent, then dispersed in 50 mL of deionized water, and freeze-dried to obtain mPEG-FBA powder.

[0132] Step 3: Synthesis of superparamagnetic ferric oxide nanoparticles (SPION): get 0.81g ferric chloride hexahydrate (FeCl 3 ·6H 2 O), 0.556g ferrous sulfate heptahydrate (FeSO 4 ·7H 2 O), dissolved in 15mL of deionized water, after the reaction system was fed with nitrogen for 50min, the temperature was raised to 75°C, and 3mL of ammonia water (the mass concentration was 35% NH 3 ·H 2 0), mixing and stirring 30min, obtains the mixed ...

Embodiment 3

[0142] Step 1: Dissolve 8g of methoxy PEG (mPEG) and 1.8g of p-formylbenzoic acid (FBA) in 160mL of dichloromethane, add 20mL of dimethylformamide (DMF), and then add 2.48g of dicyclohexyl Carbodiimide (DCC) and 732mg 4-dimethylaminopyridine (DMAP), stirred for 24h.

[0143] Step 2: The system obtained in step 1 was vacuum filtered three times to remove the precipitate, and the obtained clarified system was purged with nitrogen to remove the solvent, then dispersed in 50 mL of deionized water, and freeze-dried to obtain mPEG-FBA powder.

[0144] Step 3: Synthesis of superparamagnetic ferric oxide nanoparticles (SPION): get 0.81g ferric chloride hexahydrate (FeCl 3 ·6H 2 O), 0.556g ferrous sulfate heptahydrate (FeSO 4 ·7H 2O), dissolved in 15mL of deionized water, after the reaction system was fed with nitrogen for 50min, the temperature was raised to 75°C, and 3mL of ammonia water (the mass concentration was 35% NH 3 ·H 2 0), mixing and stirring 30min, obtains the mixed l...

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Abstract

The invention discloses a magnetic composite nano material and a preparation method and application thereof. The magnetic composite nano material comprises modified magnetic nano particles and a modified macromolecular layer located on the outer surfaces of the modified magnetic nano particles, wherein the modified macromolecular layer comprises macromolecules and polymers connected with the macromolecules through pH response groups, and the response pH value of the pH response groups is smaller than 6.8. The magnetic composite nano material exists in a cluster form before reaching a tumor part, and due to the large particle size, the magnetic composite nano material is not likely to be rapidly removed by kidney metabolism; after the magnetic composite nano material reaches the tumor part,an assembly (the magnetic composite nano material) is gradually dispersed under the tumor subacid condition, the magnetic composite nano material exists in the tumor part in a small-particle-size magnetic nano particle form, and therefore the penetration depth of tumor tissue is increased; and due to falling of the surface modification polymers, surface positive charges are increased, and the nano particles are more easily taken by cells. Therefore, the pH response type magnetic composite nano material cluster enhances the MRI imaging contrast effect and improves the tissue penetration.

Description

technical field [0001] The application relates to a magnetic composite nanomaterial and its preparation method and application, belonging to the fields of nanomaterials and biomedicine. Background technique [0002] Cancer is one of the major factors that threaten human life. Cancer Research Institute pointed out that the lack of effective screening and early diagnosis is an important factor for the high number of cancer cases. Early diagnosis is an important factor affecting the prognosis of malignant tumors and a prerequisite for early treatment. [0003] Magnetic resonance imaging (MRI) is a commonly used non-invasive method for early diagnosis of tumors in clinical practice. It has the advantages of high resolution, high tissue penetration, non-invasiveness, no radiation, and low inspection cost. ability. [0004] Superparamagnetic iron oxide NPs are popular in biomedicine because they contain degradable iron elements that can be recycled by cells using biochemical pat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/59A61K49/12A61K49/00A61P35/00
CPCA61K41/0052A61K47/59A61K49/12A61K49/0019A61K49/0002A61P35/00
Inventor 沈折玉钱昆吴爱国
Owner CIXI INST OF BIOMEDICAL ENG NINGBO INST OF MATERIALS TECH & ENG CHINESE ACAD OF SCI
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