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Strain of paenibibacillus polymyxa and application of paenibibacillus polymyxa

A technology of Paenibacillus polymyxa and Bacillus lateralis, applied in the field of microorganisms, can solve the problems of fusarium wilt in vegetables, long survival time, and easy loss of resistance, so as to promote growth, inhibit the effect, and prevent vegetable wilt the effect of

Active Publication Date: 2020-07-03
武汉合缘绿色生物股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fusarium wilt is a major epidemic disease in my country. Due to its many vectors, it can be actively and passively transmitted through the natural flow, transfer and transfer of infected crop seedlings, diseased plant residues, farming tools, soil and running water; , with the adjustment of the agricultural industry structure, the facility agriculture based on greenhouses and greenhouses has developed rapidly, especially in vegetable planting. The same kind of vegetables; and the chlamydospores of the pathogenic bacteria of fusarium wilt survive in the soil for a long time, and the combination of these reasons leads to serious occurrence of fusarium wilt in vegetables and is difficult to cure
[0003] At present, for the prevention and treatment of vegetable wilt, three major measures are generally adopted: breeding resistant varieties, agricultural measures, and chemical control. However, these three methods all have some problems, such as low disease resistance of varieties and easy loss of resistance; Control agents have poor control effect in the later stage, bacteria are prone to develop drug resistance, and the negative environmental effects are obvious; agricultural measures are easily restricted by geographical conditions, and it is difficult to promote them on a large scale, etc.

Method used

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  • Strain of paenibibacillus polymyxa and application of paenibibacillus polymyxa
  • Strain of paenibibacillus polymyxa and application of paenibibacillus polymyxa
  • Strain of paenibibacillus polymyxa and application of paenibibacillus polymyxa

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, screening and identification of Paenibacillus polymyxa

[0026] S1. Experimental materials

[0027] Soil samples were collected from the vegetable planting bases in Jiangxia and Xinzhou, Wuhan, from continuous cropping land and around the vegetable root system where serious diseases occurred.

[0028] The pathogens are represented by Fusarium oxysporum specialized in cucumber, Fusarium oxysporum specialized in tomato, Fusarium oxysporum specialized in pepper, and Fusarium oxysporum specialized in wilt, all donated by the School of Plant Science and Technology of Huazhong Agricultural University.

[0029] S2. Primary screening of strains

[0030] Soil samples were spread on yeast extract medium plate by gradient dilution method, and cultured in a 35°C incubator. After 3 days of cultivation, single colonies with different morphological characteristics were picked and cultured in three zones on the yeast extract medium, and the separation and purification w...

Embodiment 2

[0040] Embodiment 2, the antibacterial experiment of Paenibacillus polymyxa HY31

[0041] S1, preparation of Paenibacillus polymyxa HY31 bacterial liquid

[0042] Paenibacillus polymyxa HY31 was inoculated in nutrient broth medium, and cultured on a shaker at 37° C. at 200 rpm for 12 hours.

[0043] S2, preparation of pathogenic bacteria block

[0044] The bacterial blocks of four kinds of pathogenic bacteria of Fusarium oxysporum specialized in cucumber, Fusarium oxysporum specialized in tomato, Fusarium oxysporum specialized in pepper, and Fusarium oxysporum specialized in wilt were inoculated in the center of PDA solid medium, 28 Cultivate for 7 days at ℃.

[0045] S3, antibacterial experiment

[0046] Take the above four pathogenic bacteria blocks (Φ10mm) and place them in the center of the PDA solid medium plate (Φ90mm), dip the edge of a sterile blank plate with a diameter of 60mm to pick up the Paenibacillus polymyxa HY31 bacterial liquid, and put it on the above PDA...

Embodiment 3

[0051] Embodiment 3, Paenibacillus polymyxa HY31 is to vegetable disease control test

[0052] S1. Inhibitory effect of Paenibacillus polymyxa HY31 fermentation broth on Cucumber specialization of Fusarium oxysporum

[0053] Paenibacillus polymyxa HY31 was inoculated in nutrient broth liquid medium, cultured on a shaker at 37°C and 200 rpm for 48 hours, and the concentration of the fermentation broth reached 10 9 cfu / mL.

[0054] To study the inhibitory effect of Paenibacillus polymyxa HY31 fermentation broth on Fusarium oxysporum Cucumber specialization, three groups of experiments were set up: control group, low-dose group and high-dose group. First, prepare 9 PDA solid medium plates, spread pathogenic bacteria on the plates, 3 plates for each group, take a sterile Oxford cup and place it in the center of the PDA plate, and place the control group, low-dose group and high-dose group in the Oxford cups respectively Add 100 μL of sterile water, 100 μL of 5-fold diluted Paeni...

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Abstract

The present invention provides a strain of paenibibacillus polymyxa and an application of the paenibibacillus polymyxa. The strain is named paenibacillus polymyxa HY31 and has deposit number is CCTCCNo: M20191102. The strain has a strong inhibitory effect on fusarium oxysporum cucumber specialization type, fusarium oxysporum tomato specialization type, fusarium oxysporum pepper specialization type and fusarium oxysporum wilt specialization type and other plant wilt disease pathogens, and besides, a strain agent prepared from the strain is applied on vegetables to prevent occurrence of wilt diseases and promote growth of the vegetables at the same time.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a strain of Paenibacillus polymyxa and its application. Background technique [0002] Fusarium wilt, also known as blight, is a plant disease caused by fungi or bacteria. It is a worldwide soil-borne disease that has a wide range of distribution, many types of bacteria, difficult control, and seriously affects crop yield and quality. Onset is sudden, and symptoms include severe spotting, wilting, or death of leaves, flowers, fruit, stems, or the entire plant. Most economically important crops such as tomato, cucumber, and cotton are infected by one or more diseases. Fusarium wilt can affect flowers, leaves, bud bags, seedlings, twigs, stems (vines) and top tips. Fusarium wilt is a major epidemic disease in my country. Due to its many vectors, it can be actively and passively transmitted through the natural flow, transfer and transfer of infected crop seedlings, diseased ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/25A01P3/00A01P21/00C12R1/01
CPCC12N1/20A01N63/00C12R2001/01C12N1/205
Inventor 莫晓静詹巧春佀国涵张业成袁庆丰徐维烈
Owner 武汉合缘绿色生物股份有限公司
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