Strain of paenibibacillus polymyxa and application of paenibibacillus polymyxa
A technology of Paenibacillus polymyxa and Bacillus lateralis, applied in the field of microorganisms, can solve the problems of fusarium wilt in vegetables, long survival time, and easy loss of resistance, so as to promote growth, inhibit the effect, and prevent vegetable wilt the effect of
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Embodiment 1
[0025] Embodiment 1, screening and identification of Paenibacillus polymyxa
[0026] S1. Experimental materials
[0027] Soil samples were collected from the vegetable planting bases in Jiangxia and Xinzhou, Wuhan, from continuous cropping land and around the vegetable root system where serious diseases occurred.
[0028] The pathogens are represented by Fusarium oxysporum specialized in cucumber, Fusarium oxysporum specialized in tomato, Fusarium oxysporum specialized in pepper, and Fusarium oxysporum specialized in wilt, all donated by the School of Plant Science and Technology of Huazhong Agricultural University.
[0029] S2. Primary screening of strains
[0030] Soil samples were spread on yeast extract medium plate by gradient dilution method, and cultured in a 35°C incubator. After 3 days of cultivation, single colonies with different morphological characteristics were picked and cultured in three zones on the yeast extract medium, and the separation and purification w...
Embodiment 2
[0040] Embodiment 2, the antibacterial experiment of Paenibacillus polymyxa HY31
[0041] S1, preparation of Paenibacillus polymyxa HY31 bacterial liquid
[0042] Paenibacillus polymyxa HY31 was inoculated in nutrient broth medium, and cultured on a shaker at 37° C. at 200 rpm for 12 hours.
[0043] S2, preparation of pathogenic bacteria block
[0044] The bacterial blocks of four kinds of pathogenic bacteria of Fusarium oxysporum specialized in cucumber, Fusarium oxysporum specialized in tomato, Fusarium oxysporum specialized in pepper, and Fusarium oxysporum specialized in wilt were inoculated in the center of PDA solid medium, 28 Cultivate for 7 days at ℃.
[0045] S3, antibacterial experiment
[0046] Take the above four pathogenic bacteria blocks (Φ10mm) and place them in the center of the PDA solid medium plate (Φ90mm), dip the edge of a sterile blank plate with a diameter of 60mm to pick up the Paenibacillus polymyxa HY31 bacterial liquid, and put it on the above PDA...
Embodiment 3
[0051] Embodiment 3, Paenibacillus polymyxa HY31 is to vegetable disease control test
[0052] S1. Inhibitory effect of Paenibacillus polymyxa HY31 fermentation broth on Cucumber specialization of Fusarium oxysporum
[0053] Paenibacillus polymyxa HY31 was inoculated in nutrient broth liquid medium, cultured on a shaker at 37°C and 200 rpm for 48 hours, and the concentration of the fermentation broth reached 10 9 cfu / mL.
[0054] To study the inhibitory effect of Paenibacillus polymyxa HY31 fermentation broth on Fusarium oxysporum Cucumber specialization, three groups of experiments were set up: control group, low-dose group and high-dose group. First, prepare 9 PDA solid medium plates, spread pathogenic bacteria on the plates, 3 plates for each group, take a sterile Oxford cup and place it in the center of the PDA plate, and place the control group, low-dose group and high-dose group in the Oxford cups respectively Add 100 μL of sterile water, 100 μL of 5-fold diluted Paeni...
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