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A polypeptide for inhibiting novel coronavirus and its use

A coronavirus, a new type of technology, applied in the direction of viral peptides, viruses, antiviral agents, etc., can solve problems such as difficulties in virus prevention and control, and there is no preventive vaccine, and achieve the effect of inhibiting and controlling infection

Active Publication Date: 2021-02-09
北京中科微盾生物科技有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above characteristics have caused great difficulties for the prevention and control of the virus
There is currently no preventive vaccine and effective treatment for the virus

Method used

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  • A polypeptide for inhibiting novel coronavirus and its use
  • A polypeptide for inhibiting novel coronavirus and its use
  • A polypeptide for inhibiting novel coronavirus and its use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] In this example, Hun-7 cells and 293T cells were used to conduct an experiment on the inhibitory effect of the polypeptide 2019-nCov-P1 on the new coronavirus, which specifically included the following steps:

[0039] 1. Fake virus packaging

[0040] 1.1 Plasmid extraction

[0041] Pseudovirus system plasmid construction, the full-length 2019-nCov S protein was constructed into the PSV multiple cloning site, and the backbone plasmid pNL-LUR-E- was simultaneously transformed into DH5-a competent cells; 42 degrees Celsius, heat shock for 90 seconds Afterwards, spread the LB plate, the concentration of ampicillin is 5 micrograms per milliliter; cultivate overnight in a 37 degree incubator.

[0042] Pick a single colony and inoculate LB liquid medium with an ampicillin concentration of 5 micrograms per milliliter. Shake the bacteria overnight at 37°C.

[0043] Centrifuge at 12000 for 10 minutes, collect the bacteria, and extract the plasmid with Tiangen Plasmid Extractio...

Embodiment 2

[0062] The difference between this example and Example 1 is that the polypeptide 2019-nCov-P1 is replaced by the polypeptide 2019-nCov-P2.

[0063] The inhibitory efficiency of 2019-nCov-P2 on the new coronavirus is as follows: figure 2 shown.

Embodiment 3

[0065] Elisa detection of peptide binding to HR2

[0066] The HR2 polypeptide was coated onto a 96-well plate. The method is as follows, go to the polystyrene Elisa plate to equilibrate at room temperature. Dilute the HR2 polypeptide with the coating solution to a final concentration of 0.1 microgram per microliter. Coating liquid composition: 0.1696 g of anhydrous sodium carbonate, 0.2856 g of sodium bicarbonate, dissolved in 100 ml of deionized water, and added with a final concentration of 1% SDS. Add 100 microliters of protein dilution to each well. overnight at 4°C. Discard the coating solution, wash with PBST, add 300 microliters to each well, let it stand for 10 minutes, discard the washing solution, fill up the washing solution, repeat 5 times, and drain the washing solution. Washing liquid PBST: 8 grams of sodium chloride, 2.9 grams of disodium hydrogen phosphate 12 hydrate, 0.2 grams of potassium chloride, 0.24 grams of potassium dihydrogen phosphate, 0.5 millili...

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PUM

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Abstract

The present invention provides a polypeptide for inhibiting novel coronavirus, which belongs to the field of biomedicine, and the polypeptide is 2019-nCov-P1 or 2019-nCov-P2, respectively having amino acids as shown in SEQ ID NO: 1 or SEQ ID NO: 2 sequence. The polypeptide can specifically bind to the HR2 region of the S protein of COVID-19, thereby blocking the binding of the virus to cells, and effectively inhibiting and controlling the infection of the virus. The present invention also provides the application of the above polypeptide in the preparation of drugs for preventing and / or treating COVID‑19.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a novel coronavirus invasion-inhibiting polypeptide, in particular to the application of the polypeptide and its derivatives in the preparation of drugs for the prevention and / or treatment of COVID-19. Background technique [0002] Research shows that COVID-19 is a new type of coronavirus that uses the same cell receptor ACE-2 as the SARS virus, and the virus persists in the environment for a long time. Compared with the SARS virus, the transmission of COVID-19 is more stealthy, with various transmission routes and a longer incubation period. The above-mentioned characteristics have caused great difficulties for the prevention and control of the virus. Currently there is no preventive vaccine and effective treatment for this virus. Therefore, effective prevention methods and treatment measures for this virus need to be developed urgently. Contents of the invention [0003] In order t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/165A61K38/16A61P31/14
CPCA61K38/00A61P31/14C07K14/005C12N2770/20022C12N2770/20033
Inventor 岳少恒任金成
Owner 北京中科微盾生物科技有限责任公司
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