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Antidiabetic and anti-oxidative compound herbal preparation for treating diabetes mellitus

A technology of compound prescriptions and herbal medicines, applied in the direction of anti-toxins, medical preparations containing active ingredients, metabolic diseases, etc., can solve the problems of side effects of drugs, and achieve the effect of reducing the recurrence of diabetes and reducing the activity

Inactive Publication Date: 2020-06-26
SHANDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Currently, there are many synthetic drugs on the market to treat diabetes, but these drugs have side effects

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Analysis of active ingredients in compound herbal preparations:

[0016] 1. Qualitative analysis of anthraquinone compounds: Take 0.5g of compound herbal medicine extract, put it into a test tube containing 5mL of chloroform, extract it on a shaking table for 5 minutes, then filter it, add the same volume of 10% ammonia water to the filtrate, mix it, place it, and observe The color of the water layer, if it is pink, it is positive.

[0017] 2. Qualitative analysis of flavonoids: take 0.5g compound herbal extract, put it into a test tube containing 10mL ethyl acetate, heat it with hot steam for three minutes and filter it, take 4mL of the filtrate in the test tube, add 1mL of diluted ammonia to the filtrate Solution, mix well and place it, observe the color of the solution, if it is yellow, it is positive.

[0018] 3. Qualitative analysis of saponin: Take 2g of the compound herbal medicine extract, place it in a test tube filled with 20mL of distilled water, take a boil...

Embodiment 2

[0025] Determination of antioxidant substances in herbal compound preparations in vitro:

[0026] 1. Determination of total antioxidant activity by β-carotene removal method: Take 1 mL of β-carotene solution (chloroform solution containing 0.2 mg / mL β-carotene) and put it into a circle containing 25 µL of linoleic acid and 200 mg of Tween 20. In the bottom flask, chloroform was removed by rotary evaporation, and 50ml of distilled water was added to the flask, which was shaken vigorously. Take 2.5mL of the above solution and place it in a cuvette with a cover, and add 200 µL of herbal compound preparation extract solution (1 mg / mL), measure the absorbance value A0 at 470nm, and then place the cuvette in a 50°C incubator After reacting for 3 hours, measure ƛ470nm again as At, and use distilled water as a negative control to measure the absorbance value ƛ470nm as A00 and A0t at 0h and 3h of reaction, and use BHT as a positive control.

[0027] The formula for antioxidant activit...

Embodiment 3

[0040] Determination of antidiabetic activity of herbal compound preparation in vitro:

[0041] Inhibitory effect test of herbal compound preparation extract on α-amylase and α-glucosidase

[0042]Treatment of the herbal compound preparation extract: Weigh the herbal compound preparation extract and dissolve it in dimethyl sulfoxide (DMSO (v / v1:1)) to prepare a solution with a concentration of 0-4mg / ml for later use.

[0043] Test of the inhibitory effect of herbal compound preparations on α-amylase: Take 500 μl of the above-mentioned solutions of each concentration in a test tube, add 0.5 mg / ml α-amylase (dissolved in 0.02M, pH 6.9 phosphate buffer) to the test tube 500 μl of the solution was incubated at 25°C for 10 minutes, and 500 μl of 1% (dissolved in 0.02M, pH 6.9 phosphate buffer) starch solution was added again, incubated at 25°C for 10 minutes, and the reaction was terminated by adding a chromogen. Heat the test tube in boiling water for 5 min, then cool to room tem...

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PUM

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Abstract

The invention discloses an antidiabetic and anti-oxidative compound herbal extract for treating diabetes mellitus and application thereof. The extract is prepared from chlorophytum comosum roots, seeds of Anders hygrophila salicifolia, seeds of mucuna pruriens, mimosa pudica seeds, Acacia senegal gum, radix astragali, seed coat of semen plantaginis, bombax ceiba gum and roots of eurycoma longifolia through extraction and concentration of alcohol, methanol, acetone and water. In vitro tests verify that the compound herbal preparation extract has a cleaning effect on beta-carotene, A-BTS<+> freeradical and DPPH free radical, and has a remarkable oxidation resistance in comparison with artificially synthesized BHT antioxidant. The compound herbal extract has an inhibiting effect on alpha-amylase and alpha-glucosidase. The herbal compound preparation extract has a remarkable inhibiting effect in comparison with medicinal acarbose specifically for treating hyperglycemia. Therefore, the herbal compound extract has potential values on development of antidiabetic and anti-oxidative medicines for treating diabetes mellitus.

Description

technical field [0001] The invention belongs to the field of plant extraction, and in particular relates to an anti-glycemic and anti-oxidation compound herbal preparation for treating diabetes. Background technique [0002] With the characteristics of dietary changes and diversity, the incidence of obesity and diabetes in the world is increasing day by day. Diabetes is a disease caused by endocrine disorders, and it is becoming more and more common in the world. According to the statistics of the United Nations Health Organization, a total of 171 million people had diabetes in 2000, and it is estimated that by 2025, this figure may increase to 311 million. An increase or decrease in blood sugar concentration is a disease caused by the absence or disorder of pancreatic cells. According to research, in diabetic animals, it was found that the oxidative stress response caused by the excessive generation of reactive oxygen species has an important relationship with the cardiov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/896A61P3/10A61P39/06
CPCA61K36/896A61K36/19A61K36/48A61K36/481A61K36/68A61K36/185A61K36/77A61P3/10A61P39/06A61K2236/333
Inventor 宋元达赛德侯赛因杨俊换杨武
Owner SHANDONG UNIV OF TECH
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