Method for preparing pancreatic stone protein antibody and method for detecting PSP (pancreatic stone protein)

Abstract

The invention relates to a method for preparing a pancreatic stone protein antibody and a method for detecting PSP (pancreatic stone protein), and belongs to the technical field of gene recombinationof bioengineering. The invention aims to provide the method for preparing the pancreatic stone protein antibody and the method for detecting PSP (pancreatic stone protein), so that a specific PSP immunogen is prepared, a PSP monoclonal antibody or polyclonal antibody is produced, the PSP detecting method is established, and a PSP in vitro diagnosis reagent kit is established. A tag-PSP recombinantprotein carrier is constructed through a gene recombination technique, a tag-PSP recombinant protein is expressed by bacteria, yeast, insect cells, animal cells or human cells, and the tag-PSP recombinant protein is purified to be used as a PSP recombinant protein immunogen. The method disclosed by the invention is used for detecting rise of the level of the PSP in blood, and can provide a reagent kit which can diagnose the generation of bacterial septicemia early, quickly and accurately, guide treatment of septicemia, monitor the progress of the septicemia and quickly detect the PSP by an immunology method.

Description

technical field [0001] The invention belongs to the technical field of gene recombination of bioengineering. Background technique [0002] Sepsis is a potentially life-threatening state. It is mainly caused by bacterial, viral and fungal infections that cause systemic reactions in the body. Sepsis further develops into septic shock, and blood pressure drops sharply, which can quickly lead to vital organ failure and death. [0003] Early diagnosis of sepsis is the key to saving lives. For every hour of delay in treatment of sepsis, the mortality rate increases by 8%. It is necessary to provide earlier, faster and more accurate diagnosis of sepsis to greatly reduce sepsis deaths. [0004] Bacterial infection induces the production of host proteins, such as IL-6, PCT, and CRP, and detection of these host proteins is used to support the diagnosis of bacterial infection-induced sepsis. CRP is not specific for the diagnosis of sepsis because CRP levels are also elevated in inflam...

AI Technical Summary

Problems solved by technology

PCT is a relatively specific indicator for the diagnosis of sepsis, but some patients without infection after surgery, and PCT levels in trauma patients increased; while in the early stage of bacterial infection, PCT levels did not increase in some patients, indicating that PCT cannot be used as the only indicator for judging the occurrence of bacterial sepsis. Searching for new bacteria-induced host protein indicators to improve the accuracy of early diagnosis of bacterial sepsis

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